Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acids

CRISPR-Cas-based diagnostics have the potential to elevate nucleic acid detection. CRISPR-Cas systems can be combined with a pre-amplification step in a one-pot reaction to simplify the workflow and reduce carryover contamination. Here, we report an engineered Cas12b with improved thermostability th...

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Detalles Bibliográficos
Autores principales: Nguyen, Long T., Rananaware, Santosh R., Yang, Lilia G., Macaluso, Nicolas C., Ocana-Ortiz, Julio E., Meister, Katelyn S., Pizzano, Brianna L.M., Sandoval, Luke Samuel W., Hautamaki, Raymond C., Fang, Zoe R., Joseph, Sara M., Shoemaker, Grace M., Carman, Dylan R., Chang, Liwei, Rakestraw, Noah R., Zachary, Jon F., Guerra, Sebastian, Perez, Alberto, Jain, Piyush K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10213852/
https://www.ncbi.nlm.nih.gov/pubmed/37160120
http://dx.doi.org/10.1016/j.xcrm.2023.101037

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