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Effect of fluvalinate on the expression profile of circular RNA in brain tissue of Apis mellifera ligustica workers

Fluvalinate is widely used in apiculture as an acaricide for removing Varroa mites, but there have been growing concerns about the negative effects of fluvalinate on honeybees in recent years. Previous research revealed changes in the miRNA and mRNA expression profiles of Apis mellifera ligustica br...

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Autores principales: Xueqing, Shan, Delong, Lou, Guizhi, Wang, Yunhan, Fan, Liuxu, Yang, Tianle, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10213878/
https://www.ncbi.nlm.nih.gov/pubmed/37252656
http://dx.doi.org/10.3389/fgene.2023.1185952
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author Xueqing, Shan
Delong, Lou
Guizhi, Wang
Yunhan, Fan
Liuxu, Yang
Tianle, Chao
author_facet Xueqing, Shan
Delong, Lou
Guizhi, Wang
Yunhan, Fan
Liuxu, Yang
Tianle, Chao
author_sort Xueqing, Shan
collection PubMed
description Fluvalinate is widely used in apiculture as an acaricide for removing Varroa mites, but there have been growing concerns about the negative effects of fluvalinate on honeybees in recent years. Previous research revealed changes in the miRNA and mRNA expression profiles of Apis mellifera ligustica brain tissues during fluvalinate exposure, as well as key genes and pathways. The role of circRNAs in this process, however, is unknown. The goal of this study was to discover the fluvalinate-induced changes in circular RNA (circRNA) expression profiles of brain tissue of A. mellifera ligustica workers. A total of 10,780 circRNAs were detected in A. mellifera ligustica brain tissue, of which eight were differentially expressed between at least two of the four time periods before and after fluvalinate administration, and six circRNAs were experimentally verified to be structurally correct, and their expression patterns were consistent with transcriptome sequencing results. Furthermore, ceRNA analysis revealed that five differentially expressed circRNAs (DECs) (novel_circ_012139, novel_circ_011690, novel_circ_002628, novel_circ_004765, and novel_circ_010008) were primarily involved in apoptosis-related functions by competitive binding with miRNAs. This study discovered changes in the circRNA expression profile of A. mellifera ligustica brain tissue caused by fluvalinate exposure, and it provides a useful reference for the biological function study of circRNAs in A. mellifera ligustica.
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spelling pubmed-102138782023-05-27 Effect of fluvalinate on the expression profile of circular RNA in brain tissue of Apis mellifera ligustica workers Xueqing, Shan Delong, Lou Guizhi, Wang Yunhan, Fan Liuxu, Yang Tianle, Chao Front Genet Genetics Fluvalinate is widely used in apiculture as an acaricide for removing Varroa mites, but there have been growing concerns about the negative effects of fluvalinate on honeybees in recent years. Previous research revealed changes in the miRNA and mRNA expression profiles of Apis mellifera ligustica brain tissues during fluvalinate exposure, as well as key genes and pathways. The role of circRNAs in this process, however, is unknown. The goal of this study was to discover the fluvalinate-induced changes in circular RNA (circRNA) expression profiles of brain tissue of A. mellifera ligustica workers. A total of 10,780 circRNAs were detected in A. mellifera ligustica brain tissue, of which eight were differentially expressed between at least two of the four time periods before and after fluvalinate administration, and six circRNAs were experimentally verified to be structurally correct, and their expression patterns were consistent with transcriptome sequencing results. Furthermore, ceRNA analysis revealed that five differentially expressed circRNAs (DECs) (novel_circ_012139, novel_circ_011690, novel_circ_002628, novel_circ_004765, and novel_circ_010008) were primarily involved in apoptosis-related functions by competitive binding with miRNAs. This study discovered changes in the circRNA expression profile of A. mellifera ligustica brain tissue caused by fluvalinate exposure, and it provides a useful reference for the biological function study of circRNAs in A. mellifera ligustica. Frontiers Media S.A. 2023-05-11 /pmc/articles/PMC10213878/ /pubmed/37252656 http://dx.doi.org/10.3389/fgene.2023.1185952 Text en Copyright © 2023 Xueqing, Delong, Guizhi, Yunhan, Liuxu and Tianle. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Xueqing, Shan
Delong, Lou
Guizhi, Wang
Yunhan, Fan
Liuxu, Yang
Tianle, Chao
Effect of fluvalinate on the expression profile of circular RNA in brain tissue of Apis mellifera ligustica workers
title Effect of fluvalinate on the expression profile of circular RNA in brain tissue of Apis mellifera ligustica workers
title_full Effect of fluvalinate on the expression profile of circular RNA in brain tissue of Apis mellifera ligustica workers
title_fullStr Effect of fluvalinate on the expression profile of circular RNA in brain tissue of Apis mellifera ligustica workers
title_full_unstemmed Effect of fluvalinate on the expression profile of circular RNA in brain tissue of Apis mellifera ligustica workers
title_short Effect of fluvalinate on the expression profile of circular RNA in brain tissue of Apis mellifera ligustica workers
title_sort effect of fluvalinate on the expression profile of circular rna in brain tissue of apis mellifera ligustica workers
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10213878/
https://www.ncbi.nlm.nih.gov/pubmed/37252656
http://dx.doi.org/10.3389/fgene.2023.1185952
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