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Caffeine-induced release of small molecules from DNA nanostructures

Several planar aromatic molecules are known to intercalate between base pairs of double-stranded DNA. This mode of interaction has been used to stain DNA as well as to load drug molecules onto DNA-based nanostructures. Some small molecules are also known to induce deintercalation in double-stranded...

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Autores principales: Madhanagopal, Bharath Raj, Chen, Sabrina, Platt, Ché-Doni, Chandrasekaran, Arun Richard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214285/
https://www.ncbi.nlm.nih.gov/pubmed/37250306
http://dx.doi.org/10.1016/j.isci.2023.106564
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author Madhanagopal, Bharath Raj
Chen, Sabrina
Platt, Ché-Doni
Chandrasekaran, Arun Richard
author_facet Madhanagopal, Bharath Raj
Chen, Sabrina
Platt, Ché-Doni
Chandrasekaran, Arun Richard
author_sort Madhanagopal, Bharath Raj
collection PubMed
description Several planar aromatic molecules are known to intercalate between base pairs of double-stranded DNA. This mode of interaction has been used to stain DNA as well as to load drug molecules onto DNA-based nanostructures. Some small molecules are also known to induce deintercalation in double-stranded DNA, one such molecule being caffeine. Here, we compared the ability of caffeine to cause deintercalation of ethidium bromide, a representative DNA intercalator, from duplex DNA and three DNA motifs of increasing structural complexity (four-way junction, double crossover motif, and DNA tensegrity triangle). We found that caffeine impedes the binding of ethidium bromide in all these structures to the same extent, with some differences in deintercalation profiles. Our results can be useful in the design of DNA nanocarriers for intercalating drugs, where drug release can be chemically stimulated by other small molecules.
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spelling pubmed-102142852023-05-27 Caffeine-induced release of small molecules from DNA nanostructures Madhanagopal, Bharath Raj Chen, Sabrina Platt, Ché-Doni Chandrasekaran, Arun Richard iScience Article Several planar aromatic molecules are known to intercalate between base pairs of double-stranded DNA. This mode of interaction has been used to stain DNA as well as to load drug molecules onto DNA-based nanostructures. Some small molecules are also known to induce deintercalation in double-stranded DNA, one such molecule being caffeine. Here, we compared the ability of caffeine to cause deintercalation of ethidium bromide, a representative DNA intercalator, from duplex DNA and three DNA motifs of increasing structural complexity (four-way junction, double crossover motif, and DNA tensegrity triangle). We found that caffeine impedes the binding of ethidium bromide in all these structures to the same extent, with some differences in deintercalation profiles. Our results can be useful in the design of DNA nanocarriers for intercalating drugs, where drug release can be chemically stimulated by other small molecules. Elsevier 2023-04-01 /pmc/articles/PMC10214285/ /pubmed/37250306 http://dx.doi.org/10.1016/j.isci.2023.106564 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Madhanagopal, Bharath Raj
Chen, Sabrina
Platt, Ché-Doni
Chandrasekaran, Arun Richard
Caffeine-induced release of small molecules from DNA nanostructures
title Caffeine-induced release of small molecules from DNA nanostructures
title_full Caffeine-induced release of small molecules from DNA nanostructures
title_fullStr Caffeine-induced release of small molecules from DNA nanostructures
title_full_unstemmed Caffeine-induced release of small molecules from DNA nanostructures
title_short Caffeine-induced release of small molecules from DNA nanostructures
title_sort caffeine-induced release of small molecules from dna nanostructures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214285/
https://www.ncbi.nlm.nih.gov/pubmed/37250306
http://dx.doi.org/10.1016/j.isci.2023.106564
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