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Genotyping of dengue virus from infected tissue samples embedded in paraffin

Dengue has become one of the vector-borne diseases that affect humans worldwide. In Latin American countries, Colombia is historically one of the most affected by epidemics of this flavivirus. The underreporting of signs and symptoms of probable cases of dengue, the lack of characterization of the s...

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Autores principales: Rivera, Jorge Alonso, Rengifo, Aura Caterine, Rosales-Munar, Alicia, Díaz-Herrera, Taylor H., Ciro, José Usme, Parra, Edgar, Alvarez-Díaz, Diego A., Laiton-Donato, Katherine, Caldas, María Leonor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214625/
https://www.ncbi.nlm.nih.gov/pubmed/37231481
http://dx.doi.org/10.1186/s12985-023-02072-5
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author Rivera, Jorge Alonso
Rengifo, Aura Caterine
Rosales-Munar, Alicia
Díaz-Herrera, Taylor H.
Ciro, José Usme
Parra, Edgar
Alvarez-Díaz, Diego A.
Laiton-Donato, Katherine
Caldas, María Leonor
author_facet Rivera, Jorge Alonso
Rengifo, Aura Caterine
Rosales-Munar, Alicia
Díaz-Herrera, Taylor H.
Ciro, José Usme
Parra, Edgar
Alvarez-Díaz, Diego A.
Laiton-Donato, Katherine
Caldas, María Leonor
author_sort Rivera, Jorge Alonso
collection PubMed
description Dengue has become one of the vector-borne diseases that affect humans worldwide. In Latin American countries, Colombia is historically one of the most affected by epidemics of this flavivirus. The underreporting of signs and symptoms of probable cases of dengue, the lack of characterization of the serotypes of the infection, and the few detailed studies of postmortem necropsies of patients are among other conditions that have delayed progress in the knowledge of the pathogenesis of the disease. This study presents the results of fragment sequencing assays on paraffin-embedded tissue samples from fatal DENV cases during the 2010 epidemic in Colombia. We found that the predominant serotype was DENV-2, with the Asian/American genotype of lineages 1 and 2. This work is one of the few reports of the circulating genotypes of dengue during the 2010 epidemic in Colombia, one of the most lethal dates in the country's history. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-023-02072-5.
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spelling pubmed-102146252023-05-27 Genotyping of dengue virus from infected tissue samples embedded in paraffin Rivera, Jorge Alonso Rengifo, Aura Caterine Rosales-Munar, Alicia Díaz-Herrera, Taylor H. Ciro, José Usme Parra, Edgar Alvarez-Díaz, Diego A. Laiton-Donato, Katherine Caldas, María Leonor Virol J Brief Report Dengue has become one of the vector-borne diseases that affect humans worldwide. In Latin American countries, Colombia is historically one of the most affected by epidemics of this flavivirus. The underreporting of signs and symptoms of probable cases of dengue, the lack of characterization of the serotypes of the infection, and the few detailed studies of postmortem necropsies of patients are among other conditions that have delayed progress in the knowledge of the pathogenesis of the disease. This study presents the results of fragment sequencing assays on paraffin-embedded tissue samples from fatal DENV cases during the 2010 epidemic in Colombia. We found that the predominant serotype was DENV-2, with the Asian/American genotype of lineages 1 and 2. This work is one of the few reports of the circulating genotypes of dengue during the 2010 epidemic in Colombia, one of the most lethal dates in the country's history. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-023-02072-5. BioMed Central 2023-05-25 /pmc/articles/PMC10214625/ /pubmed/37231481 http://dx.doi.org/10.1186/s12985-023-02072-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Brief Report
Rivera, Jorge Alonso
Rengifo, Aura Caterine
Rosales-Munar, Alicia
Díaz-Herrera, Taylor H.
Ciro, José Usme
Parra, Edgar
Alvarez-Díaz, Diego A.
Laiton-Donato, Katherine
Caldas, María Leonor
Genotyping of dengue virus from infected tissue samples embedded in paraffin
title Genotyping of dengue virus from infected tissue samples embedded in paraffin
title_full Genotyping of dengue virus from infected tissue samples embedded in paraffin
title_fullStr Genotyping of dengue virus from infected tissue samples embedded in paraffin
title_full_unstemmed Genotyping of dengue virus from infected tissue samples embedded in paraffin
title_short Genotyping of dengue virus from infected tissue samples embedded in paraffin
title_sort genotyping of dengue virus from infected tissue samples embedded in paraffin
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214625/
https://www.ncbi.nlm.nih.gov/pubmed/37231481
http://dx.doi.org/10.1186/s12985-023-02072-5
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