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Genotyping of dengue virus from infected tissue samples embedded in paraffin
Dengue has become one of the vector-borne diseases that affect humans worldwide. In Latin American countries, Colombia is historically one of the most affected by epidemics of this flavivirus. The underreporting of signs and symptoms of probable cases of dengue, the lack of characterization of the s...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214625/ https://www.ncbi.nlm.nih.gov/pubmed/37231481 http://dx.doi.org/10.1186/s12985-023-02072-5 |
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author | Rivera, Jorge Alonso Rengifo, Aura Caterine Rosales-Munar, Alicia Díaz-Herrera, Taylor H. Ciro, José Usme Parra, Edgar Alvarez-Díaz, Diego A. Laiton-Donato, Katherine Caldas, María Leonor |
author_facet | Rivera, Jorge Alonso Rengifo, Aura Caterine Rosales-Munar, Alicia Díaz-Herrera, Taylor H. Ciro, José Usme Parra, Edgar Alvarez-Díaz, Diego A. Laiton-Donato, Katherine Caldas, María Leonor |
author_sort | Rivera, Jorge Alonso |
collection | PubMed |
description | Dengue has become one of the vector-borne diseases that affect humans worldwide. In Latin American countries, Colombia is historically one of the most affected by epidemics of this flavivirus. The underreporting of signs and symptoms of probable cases of dengue, the lack of characterization of the serotypes of the infection, and the few detailed studies of postmortem necropsies of patients are among other conditions that have delayed progress in the knowledge of the pathogenesis of the disease. This study presents the results of fragment sequencing assays on paraffin-embedded tissue samples from fatal DENV cases during the 2010 epidemic in Colombia. We found that the predominant serotype was DENV-2, with the Asian/American genotype of lineages 1 and 2. This work is one of the few reports of the circulating genotypes of dengue during the 2010 epidemic in Colombia, one of the most lethal dates in the country's history. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-023-02072-5. |
format | Online Article Text |
id | pubmed-10214625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-102146252023-05-27 Genotyping of dengue virus from infected tissue samples embedded in paraffin Rivera, Jorge Alonso Rengifo, Aura Caterine Rosales-Munar, Alicia Díaz-Herrera, Taylor H. Ciro, José Usme Parra, Edgar Alvarez-Díaz, Diego A. Laiton-Donato, Katherine Caldas, María Leonor Virol J Brief Report Dengue has become one of the vector-borne diseases that affect humans worldwide. In Latin American countries, Colombia is historically one of the most affected by epidemics of this flavivirus. The underreporting of signs and symptoms of probable cases of dengue, the lack of characterization of the serotypes of the infection, and the few detailed studies of postmortem necropsies of patients are among other conditions that have delayed progress in the knowledge of the pathogenesis of the disease. This study presents the results of fragment sequencing assays on paraffin-embedded tissue samples from fatal DENV cases during the 2010 epidemic in Colombia. We found that the predominant serotype was DENV-2, with the Asian/American genotype of lineages 1 and 2. This work is one of the few reports of the circulating genotypes of dengue during the 2010 epidemic in Colombia, one of the most lethal dates in the country's history. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-023-02072-5. BioMed Central 2023-05-25 /pmc/articles/PMC10214625/ /pubmed/37231481 http://dx.doi.org/10.1186/s12985-023-02072-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Brief Report Rivera, Jorge Alonso Rengifo, Aura Caterine Rosales-Munar, Alicia Díaz-Herrera, Taylor H. Ciro, José Usme Parra, Edgar Alvarez-Díaz, Diego A. Laiton-Donato, Katherine Caldas, María Leonor Genotyping of dengue virus from infected tissue samples embedded in paraffin |
title | Genotyping of dengue virus from infected tissue samples embedded in paraffin |
title_full | Genotyping of dengue virus from infected tissue samples embedded in paraffin |
title_fullStr | Genotyping of dengue virus from infected tissue samples embedded in paraffin |
title_full_unstemmed | Genotyping of dengue virus from infected tissue samples embedded in paraffin |
title_short | Genotyping of dengue virus from infected tissue samples embedded in paraffin |
title_sort | genotyping of dengue virus from infected tissue samples embedded in paraffin |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214625/ https://www.ncbi.nlm.nih.gov/pubmed/37231481 http://dx.doi.org/10.1186/s12985-023-02072-5 |
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