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The effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients

BACKGROUND &OBJECTIVE: Y chromosome abnormalities are common in male patients with severe oligo-azoospermia. In studies with karyotype analysis and cytogenetic methods, the importance of the Y chromosome in spermatogenesis has been well understood. Deletions in the azoospermia factor (AZF) local...

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Autores principales: Emirdar, Volkan, Acet, Ferruh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Professional Medical Publications 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214791/
https://www.ncbi.nlm.nih.gov/pubmed/37250564
http://dx.doi.org/10.12669/pjms.39.3.7003
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author Emirdar, Volkan
Acet, Ferruh
author_facet Emirdar, Volkan
Acet, Ferruh
author_sort Emirdar, Volkan
collection PubMed
description BACKGROUND &OBJECTIVE: Y chromosome abnormalities are common in male patients with severe oligo-azoospermia. In studies with karyotype analysis and cytogenetic methods, the importance of the Y chromosome in spermatogenesis has been well understood. Deletions in the azoospermia factor (AZF) localized at the distal end of the Y chromosome adversely affect the spermatogenesis process. Our objective was to determine the frequency of AZF microdeletion in azoospermia patients who underwent microTESE. METHODS: In this retrospective cohort study, 806 azoospermic men attending the In Vitro Fertilization (IVF) Center for infertility treatment between 2010 and 2022 were included. AZF deletion screening was conducted in all patients included in the study. Azoospermic patients with and without Y microdeletion were matched with the female’s age, cause of infertility, number of oocytes retrieved and number of metaphase II (MII) oocytes produced and compared. The primary outcome was the live birth rate (LBR). Pregnancy rate (PR) and clinical pregnancy rates (CPR) were secondary outcomes. RESULTS: We detected Y microdeletion in 55 (6.82%) of 806 infertile azoospermic men and 35 of them included in the study. Although the required gonadotropin dose and the total number of retrieved oocytes were similar, clinical pregnancy rates and live birth rates were found to be significantly lower in the microdeletion patient group (21.6% vs. 43%, p<0.05; and 18.9% vs. 36%, p<0.05, respectively). CONCLUSIONS: Poor sperm quality in AZF microdeletion patients complicates the selection of appropriate sperm for ICSI. Therefore, it leads to a decrease in embryonic development, fertilization and pregnancy results. In order to select the best sperm for the use in ICSI procedure in this patient population, intracytoplasmic morphologically selected sperm injection (IMSI) method can be preferred to improve the cycle outcomes.
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spelling pubmed-102147912023-05-27 The effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients Emirdar, Volkan Acet, Ferruh Pak J Med Sci Original Article BACKGROUND &OBJECTIVE: Y chromosome abnormalities are common in male patients with severe oligo-azoospermia. In studies with karyotype analysis and cytogenetic methods, the importance of the Y chromosome in spermatogenesis has been well understood. Deletions in the azoospermia factor (AZF) localized at the distal end of the Y chromosome adversely affect the spermatogenesis process. Our objective was to determine the frequency of AZF microdeletion in azoospermia patients who underwent microTESE. METHODS: In this retrospective cohort study, 806 azoospermic men attending the In Vitro Fertilization (IVF) Center for infertility treatment between 2010 and 2022 were included. AZF deletion screening was conducted in all patients included in the study. Azoospermic patients with and without Y microdeletion were matched with the female’s age, cause of infertility, number of oocytes retrieved and number of metaphase II (MII) oocytes produced and compared. The primary outcome was the live birth rate (LBR). Pregnancy rate (PR) and clinical pregnancy rates (CPR) were secondary outcomes. RESULTS: We detected Y microdeletion in 55 (6.82%) of 806 infertile azoospermic men and 35 of them included in the study. Although the required gonadotropin dose and the total number of retrieved oocytes were similar, clinical pregnancy rates and live birth rates were found to be significantly lower in the microdeletion patient group (21.6% vs. 43%, p<0.05; and 18.9% vs. 36%, p<0.05, respectively). CONCLUSIONS: Poor sperm quality in AZF microdeletion patients complicates the selection of appropriate sperm for ICSI. Therefore, it leads to a decrease in embryonic development, fertilization and pregnancy results. In order to select the best sperm for the use in ICSI procedure in this patient population, intracytoplasmic morphologically selected sperm injection (IMSI) method can be preferred to improve the cycle outcomes. Professional Medical Publications 2023 /pmc/articles/PMC10214791/ /pubmed/37250564 http://dx.doi.org/10.12669/pjms.39.3.7003 Text en Copyright: © Pakistan Journal of Medical Sciences https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0 (https://creativecommons.org/licenses/by/3.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Emirdar, Volkan
Acet, Ferruh
The effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients
title The effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients
title_full The effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients
title_fullStr The effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients
title_full_unstemmed The effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients
title_short The effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients
title_sort effect of azoospermia factor microdeletions on intracytoplasmic sperm injection results in azoospermia patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214791/
https://www.ncbi.nlm.nih.gov/pubmed/37250564
http://dx.doi.org/10.12669/pjms.39.3.7003
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