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Establishment of a novel protocol for formalin-fixed paraffin-embedded organoids and spheroids
Three-dimensional (3D) cell culture models such as spheroids and organoids are widely used in the field of experimental biology. To analyze these 3D experimental models, formalin-fixed paraffin-embedded (FFPE) sections are superior to whole-mount imaging for some experimental purposes, such as explo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214850/ https://www.ncbi.nlm.nih.gov/pubmed/37191105 http://dx.doi.org/10.1242/bio.059882 |
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author | Yoshimoto, Shohei Taguchi, Masahide Sumi, Satoko Oka, Kyoko Okamura, Kazuhiko |
author_facet | Yoshimoto, Shohei Taguchi, Masahide Sumi, Satoko Oka, Kyoko Okamura, Kazuhiko |
author_sort | Yoshimoto, Shohei |
collection | PubMed |
description | Three-dimensional (3D) cell culture models such as spheroids and organoids are widely used in the field of experimental biology. To analyze these 3D experimental models, formalin-fixed paraffin-embedded (FFPE) sections are superior to whole-mount imaging for some experimental purposes, such as exploring samples with a depth limitation of primary antibody penetration immunohistochemically. However, tiny 3D cell culture samples are difficult to embed in paraffin and acquire appropriate sections. In this report, we optimized a protocol of paraffin embedding for spheroids and organoids. In addition, we compared FFPE sections with frozen sections in ratio of sample collection and section condition after staining, and could reproduce improved results reliably. The protocol we established could be widely used in many laboratories and become a useful technique for analyzing spheroids and organoids. |
format | Online Article Text |
id | pubmed-10214850 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-102148502023-05-27 Establishment of a novel protocol for formalin-fixed paraffin-embedded organoids and spheroids Yoshimoto, Shohei Taguchi, Masahide Sumi, Satoko Oka, Kyoko Okamura, Kazuhiko Biol Open Methods & Techniques Three-dimensional (3D) cell culture models such as spheroids and organoids are widely used in the field of experimental biology. To analyze these 3D experimental models, formalin-fixed paraffin-embedded (FFPE) sections are superior to whole-mount imaging for some experimental purposes, such as exploring samples with a depth limitation of primary antibody penetration immunohistochemically. However, tiny 3D cell culture samples are difficult to embed in paraffin and acquire appropriate sections. In this report, we optimized a protocol of paraffin embedding for spheroids and organoids. In addition, we compared FFPE sections with frozen sections in ratio of sample collection and section condition after staining, and could reproduce improved results reliably. The protocol we established could be widely used in many laboratories and become a useful technique for analyzing spheroids and organoids. The Company of Biologists Ltd 2023-05-16 /pmc/articles/PMC10214850/ /pubmed/37191105 http://dx.doi.org/10.1242/bio.059882 Text en © 2023. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Methods & Techniques Yoshimoto, Shohei Taguchi, Masahide Sumi, Satoko Oka, Kyoko Okamura, Kazuhiko Establishment of a novel protocol for formalin-fixed paraffin-embedded organoids and spheroids |
title | Establishment of a novel protocol for formalin-fixed paraffin-embedded organoids and spheroids |
title_full | Establishment of a novel protocol for formalin-fixed paraffin-embedded organoids and spheroids |
title_fullStr | Establishment of a novel protocol for formalin-fixed paraffin-embedded organoids and spheroids |
title_full_unstemmed | Establishment of a novel protocol for formalin-fixed paraffin-embedded organoids and spheroids |
title_short | Establishment of a novel protocol for formalin-fixed paraffin-embedded organoids and spheroids |
title_sort | establishment of a novel protocol for formalin-fixed paraffin-embedded organoids and spheroids |
topic | Methods & Techniques |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214850/ https://www.ncbi.nlm.nih.gov/pubmed/37191105 http://dx.doi.org/10.1242/bio.059882 |
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