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Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance

Vancomycin and daptomycin are first-line drugs for the treatment of complicated methicillin-resistant Staphylococcus aureus (MRSA) infections, including bacteremia. However, their effectiveness is limited not only by their resistance to each antibiotic but also by their associated resistance to both...

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Autores principales: Gostev, Vladimir, Kalinogorskaya, Olga, Sopova, Julia, Sulian, Ofelia, Chulkova, Polina, Velizhanina, Maria, Tsvetkova, Irina, Ageevets, Irina, Ageevets, Vladimir, Sidorenko, Sergey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215302/
https://www.ncbi.nlm.nih.gov/pubmed/37237831
http://dx.doi.org/10.3390/antibiotics12050928
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author Gostev, Vladimir
Kalinogorskaya, Olga
Sopova, Julia
Sulian, Ofelia
Chulkova, Polina
Velizhanina, Maria
Tsvetkova, Irina
Ageevets, Irina
Ageevets, Vladimir
Sidorenko, Sergey
author_facet Gostev, Vladimir
Kalinogorskaya, Olga
Sopova, Julia
Sulian, Ofelia
Chulkova, Polina
Velizhanina, Maria
Tsvetkova, Irina
Ageevets, Irina
Ageevets, Vladimir
Sidorenko, Sergey
author_sort Gostev, Vladimir
collection PubMed
description Vancomycin and daptomycin are first-line drugs for the treatment of complicated methicillin-resistant Staphylococcus aureus (MRSA) infections, including bacteremia. However, their effectiveness is limited not only by their resistance to each antibiotic but also by their associated resistance to both drugs. It is unknown whether novel lipoglycopeptides can overcome this associated resistance. Resistant derivatives from five S. aureus strains were obtained during adaptive laboratory evolution with vancomycin and daptomycin. Both parental and derivative strains were subjected to susceptibility testing, population analysis profiles, measurements of growth rate and autolytic activity, and whole-genome sequencing. Regardless of whether vancomycin or daptomycin was selected, most of the derivatives were characterized by a reduced susceptibility to daptomycin, vancomycin, telavancin, dalbavancin, and oritavancin. Resistance to induced autolysis was observed in all derivatives. Daptomycin resistance was associated with a significant reduction in growth rate. Resistance to vancomycin was mainly associated with mutations in the genes responsible for cell wall biosynthesis, and resistance to daptomycin was associated with mutations in the genes responsible for phospholipid biosynthesis and glycerol metabolism. However, mutations in walK and mprF were detected in derivatives selected for both antibiotics.
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spelling pubmed-102153022023-05-27 Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance Gostev, Vladimir Kalinogorskaya, Olga Sopova, Julia Sulian, Ofelia Chulkova, Polina Velizhanina, Maria Tsvetkova, Irina Ageevets, Irina Ageevets, Vladimir Sidorenko, Sergey Antibiotics (Basel) Article Vancomycin and daptomycin are first-line drugs for the treatment of complicated methicillin-resistant Staphylococcus aureus (MRSA) infections, including bacteremia. However, their effectiveness is limited not only by their resistance to each antibiotic but also by their associated resistance to both drugs. It is unknown whether novel lipoglycopeptides can overcome this associated resistance. Resistant derivatives from five S. aureus strains were obtained during adaptive laboratory evolution with vancomycin and daptomycin. Both parental and derivative strains were subjected to susceptibility testing, population analysis profiles, measurements of growth rate and autolytic activity, and whole-genome sequencing. Regardless of whether vancomycin or daptomycin was selected, most of the derivatives were characterized by a reduced susceptibility to daptomycin, vancomycin, telavancin, dalbavancin, and oritavancin. Resistance to induced autolysis was observed in all derivatives. Daptomycin resistance was associated with a significant reduction in growth rate. Resistance to vancomycin was mainly associated with mutations in the genes responsible for cell wall biosynthesis, and resistance to daptomycin was associated with mutations in the genes responsible for phospholipid biosynthesis and glycerol metabolism. However, mutations in walK and mprF were detected in derivatives selected for both antibiotics. MDPI 2023-05-18 /pmc/articles/PMC10215302/ /pubmed/37237831 http://dx.doi.org/10.3390/antibiotics12050928 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gostev, Vladimir
Kalinogorskaya, Olga
Sopova, Julia
Sulian, Ofelia
Chulkova, Polina
Velizhanina, Maria
Tsvetkova, Irina
Ageevets, Irina
Ageevets, Vladimir
Sidorenko, Sergey
Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance
title Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance
title_full Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance
title_fullStr Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance
title_full_unstemmed Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance
title_short Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance
title_sort adaptive laboratory evolution of staphylococcus aureus resistance to vancomycin and daptomycin: mutation patterns and cross-resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215302/
https://www.ncbi.nlm.nih.gov/pubmed/37237831
http://dx.doi.org/10.3390/antibiotics12050928
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