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Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance
Vancomycin and daptomycin are first-line drugs for the treatment of complicated methicillin-resistant Staphylococcus aureus (MRSA) infections, including bacteremia. However, their effectiveness is limited not only by their resistance to each antibiotic but also by their associated resistance to both...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215302/ https://www.ncbi.nlm.nih.gov/pubmed/37237831 http://dx.doi.org/10.3390/antibiotics12050928 |
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author | Gostev, Vladimir Kalinogorskaya, Olga Sopova, Julia Sulian, Ofelia Chulkova, Polina Velizhanina, Maria Tsvetkova, Irina Ageevets, Irina Ageevets, Vladimir Sidorenko, Sergey |
author_facet | Gostev, Vladimir Kalinogorskaya, Olga Sopova, Julia Sulian, Ofelia Chulkova, Polina Velizhanina, Maria Tsvetkova, Irina Ageevets, Irina Ageevets, Vladimir Sidorenko, Sergey |
author_sort | Gostev, Vladimir |
collection | PubMed |
description | Vancomycin and daptomycin are first-line drugs for the treatment of complicated methicillin-resistant Staphylococcus aureus (MRSA) infections, including bacteremia. However, their effectiveness is limited not only by their resistance to each antibiotic but also by their associated resistance to both drugs. It is unknown whether novel lipoglycopeptides can overcome this associated resistance. Resistant derivatives from five S. aureus strains were obtained during adaptive laboratory evolution with vancomycin and daptomycin. Both parental and derivative strains were subjected to susceptibility testing, population analysis profiles, measurements of growth rate and autolytic activity, and whole-genome sequencing. Regardless of whether vancomycin or daptomycin was selected, most of the derivatives were characterized by a reduced susceptibility to daptomycin, vancomycin, telavancin, dalbavancin, and oritavancin. Resistance to induced autolysis was observed in all derivatives. Daptomycin resistance was associated with a significant reduction in growth rate. Resistance to vancomycin was mainly associated with mutations in the genes responsible for cell wall biosynthesis, and resistance to daptomycin was associated with mutations in the genes responsible for phospholipid biosynthesis and glycerol metabolism. However, mutations in walK and mprF were detected in derivatives selected for both antibiotics. |
format | Online Article Text |
id | pubmed-10215302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102153022023-05-27 Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance Gostev, Vladimir Kalinogorskaya, Olga Sopova, Julia Sulian, Ofelia Chulkova, Polina Velizhanina, Maria Tsvetkova, Irina Ageevets, Irina Ageevets, Vladimir Sidorenko, Sergey Antibiotics (Basel) Article Vancomycin and daptomycin are first-line drugs for the treatment of complicated methicillin-resistant Staphylococcus aureus (MRSA) infections, including bacteremia. However, their effectiveness is limited not only by their resistance to each antibiotic but also by their associated resistance to both drugs. It is unknown whether novel lipoglycopeptides can overcome this associated resistance. Resistant derivatives from five S. aureus strains were obtained during adaptive laboratory evolution with vancomycin and daptomycin. Both parental and derivative strains were subjected to susceptibility testing, population analysis profiles, measurements of growth rate and autolytic activity, and whole-genome sequencing. Regardless of whether vancomycin or daptomycin was selected, most of the derivatives were characterized by a reduced susceptibility to daptomycin, vancomycin, telavancin, dalbavancin, and oritavancin. Resistance to induced autolysis was observed in all derivatives. Daptomycin resistance was associated with a significant reduction in growth rate. Resistance to vancomycin was mainly associated with mutations in the genes responsible for cell wall biosynthesis, and resistance to daptomycin was associated with mutations in the genes responsible for phospholipid biosynthesis and glycerol metabolism. However, mutations in walK and mprF were detected in derivatives selected for both antibiotics. MDPI 2023-05-18 /pmc/articles/PMC10215302/ /pubmed/37237831 http://dx.doi.org/10.3390/antibiotics12050928 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gostev, Vladimir Kalinogorskaya, Olga Sopova, Julia Sulian, Ofelia Chulkova, Polina Velizhanina, Maria Tsvetkova, Irina Ageevets, Irina Ageevets, Vladimir Sidorenko, Sergey Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance |
title | Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance |
title_full | Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance |
title_fullStr | Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance |
title_full_unstemmed | Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance |
title_short | Adaptive Laboratory Evolution of Staphylococcus aureus Resistance to Vancomycin and Daptomycin: Mutation Patterns and Cross-Resistance |
title_sort | adaptive laboratory evolution of staphylococcus aureus resistance to vancomycin and daptomycin: mutation patterns and cross-resistance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215302/ https://www.ncbi.nlm.nih.gov/pubmed/37237831 http://dx.doi.org/10.3390/antibiotics12050928 |
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