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Biocide Susceptibility and Antimicrobial Resistance of Escherichia coli Isolated from Swine Feces, Pork Meat and Humans in Germany

Phenotypic susceptibility testing of Escherichia (E.) coli is an essential tool to gain a better understanding of the potential impact of biocide selection pressure on antimicrobial resistance. We, therefore, determined the biocide and antimicrobial susceptibility of 216 extended-spectrum β-lactamas...

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Autores principales: da Silva, David Attuy Vey, Dieckmann, Ralf, Makarewicz, Oliwia, Hartung, Anita, Bethe, Astrid, Grobbel, Mirjam, Belik, Vitaly, Pletz, Mathias W., Al Dahouk, Sascha, Neuhaus, Szilvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215396/
https://www.ncbi.nlm.nih.gov/pubmed/37237726
http://dx.doi.org/10.3390/antibiotics12050823
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author da Silva, David Attuy Vey
Dieckmann, Ralf
Makarewicz, Oliwia
Hartung, Anita
Bethe, Astrid
Grobbel, Mirjam
Belik, Vitaly
Pletz, Mathias W.
Al Dahouk, Sascha
Neuhaus, Szilvia
author_facet da Silva, David Attuy Vey
Dieckmann, Ralf
Makarewicz, Oliwia
Hartung, Anita
Bethe, Astrid
Grobbel, Mirjam
Belik, Vitaly
Pletz, Mathias W.
Al Dahouk, Sascha
Neuhaus, Szilvia
author_sort da Silva, David Attuy Vey
collection PubMed
description Phenotypic susceptibility testing of Escherichia (E.) coli is an essential tool to gain a better understanding of the potential impact of biocide selection pressure on antimicrobial resistance. We, therefore, determined the biocide and antimicrobial susceptibility of 216 extended-spectrum β-lactamase-producing (ESBL) and 177 non-ESBL E. coli isolated from swine feces, pork meat, voluntary donors and inpatients and evaluated associations between their susceptibilities. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of benzalkonium chloride, chlorhexidine digluconate (CHG), chlorocresol (PCMC), glutaraldehyde (GDA), isopropanol (IPA), octenidine dihydrochloride and sodium hypochlorite (NaOCl) showed unimodal distributions, indicating the absence of bacterial adaptation to biocides due to the acquisition of resistance mechanisms. Although MIC(95) and MBC(95) did not vary more than one doubling dilution step between isolates of porcine and human origin, significant differences in MIC and/or MBC distributions were identified for GDA, CHG, IPA, PCMC and NaOCl. Comparing non-ESBL and ESBL E. coli, significantly different MIC and/or MBC distributions were found for PCMC, CHG and GDA. Antimicrobial susceptibility testing revealed the highest frequency of resistant E. coli in the subpopulation isolated from inpatients. We observed significant but weakly positive correlations between biocide MICs and/or MBCs and antimicrobial MICs. In summary, our data indicate a rather moderate effect of biocide use on the susceptibility of E. coli to biocides and antimicrobials.
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spelling pubmed-102153962023-05-27 Biocide Susceptibility and Antimicrobial Resistance of Escherichia coli Isolated from Swine Feces, Pork Meat and Humans in Germany da Silva, David Attuy Vey Dieckmann, Ralf Makarewicz, Oliwia Hartung, Anita Bethe, Astrid Grobbel, Mirjam Belik, Vitaly Pletz, Mathias W. Al Dahouk, Sascha Neuhaus, Szilvia Antibiotics (Basel) Article Phenotypic susceptibility testing of Escherichia (E.) coli is an essential tool to gain a better understanding of the potential impact of biocide selection pressure on antimicrobial resistance. We, therefore, determined the biocide and antimicrobial susceptibility of 216 extended-spectrum β-lactamase-producing (ESBL) and 177 non-ESBL E. coli isolated from swine feces, pork meat, voluntary donors and inpatients and evaluated associations between their susceptibilities. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of benzalkonium chloride, chlorhexidine digluconate (CHG), chlorocresol (PCMC), glutaraldehyde (GDA), isopropanol (IPA), octenidine dihydrochloride and sodium hypochlorite (NaOCl) showed unimodal distributions, indicating the absence of bacterial adaptation to biocides due to the acquisition of resistance mechanisms. Although MIC(95) and MBC(95) did not vary more than one doubling dilution step between isolates of porcine and human origin, significant differences in MIC and/or MBC distributions were identified for GDA, CHG, IPA, PCMC and NaOCl. Comparing non-ESBL and ESBL E. coli, significantly different MIC and/or MBC distributions were found for PCMC, CHG and GDA. Antimicrobial susceptibility testing revealed the highest frequency of resistant E. coli in the subpopulation isolated from inpatients. We observed significant but weakly positive correlations between biocide MICs and/or MBCs and antimicrobial MICs. In summary, our data indicate a rather moderate effect of biocide use on the susceptibility of E. coli to biocides and antimicrobials. MDPI 2023-04-27 /pmc/articles/PMC10215396/ /pubmed/37237726 http://dx.doi.org/10.3390/antibiotics12050823 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Silva, David Attuy Vey
Dieckmann, Ralf
Makarewicz, Oliwia
Hartung, Anita
Bethe, Astrid
Grobbel, Mirjam
Belik, Vitaly
Pletz, Mathias W.
Al Dahouk, Sascha
Neuhaus, Szilvia
Biocide Susceptibility and Antimicrobial Resistance of Escherichia coli Isolated from Swine Feces, Pork Meat and Humans in Germany
title Biocide Susceptibility and Antimicrobial Resistance of Escherichia coli Isolated from Swine Feces, Pork Meat and Humans in Germany
title_full Biocide Susceptibility and Antimicrobial Resistance of Escherichia coli Isolated from Swine Feces, Pork Meat and Humans in Germany
title_fullStr Biocide Susceptibility and Antimicrobial Resistance of Escherichia coli Isolated from Swine Feces, Pork Meat and Humans in Germany
title_full_unstemmed Biocide Susceptibility and Antimicrobial Resistance of Escherichia coli Isolated from Swine Feces, Pork Meat and Humans in Germany
title_short Biocide Susceptibility and Antimicrobial Resistance of Escherichia coli Isolated from Swine Feces, Pork Meat and Humans in Germany
title_sort biocide susceptibility and antimicrobial resistance of escherichia coli isolated from swine feces, pork meat and humans in germany
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215396/
https://www.ncbi.nlm.nih.gov/pubmed/37237726
http://dx.doi.org/10.3390/antibiotics12050823
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