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Generation of Herbicide-Resistant Soybean by Base Editing
SIMPLE SUMMARY: Soybean is a prominent grain and oil crop in the world. The production of soybean is threatened by weed competition. Recently, CRISPR/Cas9-mediated base editing, which can cause irreversible nucleotide changes in target loci, has been widely used in basic plant science and breeding....
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215408/ https://www.ncbi.nlm.nih.gov/pubmed/37237553 http://dx.doi.org/10.3390/biology12050741 |
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author | Wei, Tao Jiang, Linjian You, Xiang Ma, Pengyu Xi, Zhen Wang, Ning Ning |
author_facet | Wei, Tao Jiang, Linjian You, Xiang Ma, Pengyu Xi, Zhen Wang, Ning Ning |
author_sort | Wei, Tao |
collection | PubMed |
description | SIMPLE SUMMARY: Soybean is a prominent grain and oil crop in the world. The production of soybean is threatened by weed competition. Recently, CRISPR/Cas9-mediated base editing, which can cause irreversible nucleotide changes in target loci, has been widely used in basic plant science and breeding. However, until now, herbicide-resistant traits in soybean have not been created by the base editor. The objective of this study was to modify different AHAS homologous alleles by base editor to generate a novel AHAS-inhibiting herbicide-resistant soybean. Our results showed that the GmAHAS4 P180S mutants have apparent resistance to AHAS-inhibiting herbicides, which will be beneficial for weed control in soybean production. ABSTRACT: Weeds cause the largest yield loss in soybean production. The development of herbicide-resistant soybean germplasm is of great significance for weed control and yield improvement. In this study, we used the cytosine base editor (BE3) to develop novel herbicide-resistant soybean. We have successfully introduced base substitutions in GmAHAS3 and GmAHAS4 and obtained a heritable transgene-free soybean with homozygous P180S mutation in GmAHAS4. The GmAHAS4 P180S mutants have apparent resistance to chlorsulfuron, flucarbazone-sodium, and flumetsulam. In particular, the resistance to chlorsulfuron was more than 100 times that of with wild type TL-1. The agronomic performance of the GmAHAS4 P180S mutants showed no significant differences to TL-1 under natural growth conditions. In addition, we developed allele-specific PCR markers for the GmAHAS4 P180S mutants, which can easily discriminate homozygous, heterozygous mutants, and wild-type plants. This study demonstrates a feasible and effective way to generate herbicide-resistant soybean by using CRISPR/Cas9-mediated base editing. |
format | Online Article Text |
id | pubmed-10215408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102154082023-05-27 Generation of Herbicide-Resistant Soybean by Base Editing Wei, Tao Jiang, Linjian You, Xiang Ma, Pengyu Xi, Zhen Wang, Ning Ning Biology (Basel) Brief Report SIMPLE SUMMARY: Soybean is a prominent grain and oil crop in the world. The production of soybean is threatened by weed competition. Recently, CRISPR/Cas9-mediated base editing, which can cause irreversible nucleotide changes in target loci, has been widely used in basic plant science and breeding. However, until now, herbicide-resistant traits in soybean have not been created by the base editor. The objective of this study was to modify different AHAS homologous alleles by base editor to generate a novel AHAS-inhibiting herbicide-resistant soybean. Our results showed that the GmAHAS4 P180S mutants have apparent resistance to AHAS-inhibiting herbicides, which will be beneficial for weed control in soybean production. ABSTRACT: Weeds cause the largest yield loss in soybean production. The development of herbicide-resistant soybean germplasm is of great significance for weed control and yield improvement. In this study, we used the cytosine base editor (BE3) to develop novel herbicide-resistant soybean. We have successfully introduced base substitutions in GmAHAS3 and GmAHAS4 and obtained a heritable transgene-free soybean with homozygous P180S mutation in GmAHAS4. The GmAHAS4 P180S mutants have apparent resistance to chlorsulfuron, flucarbazone-sodium, and flumetsulam. In particular, the resistance to chlorsulfuron was more than 100 times that of with wild type TL-1. The agronomic performance of the GmAHAS4 P180S mutants showed no significant differences to TL-1 under natural growth conditions. In addition, we developed allele-specific PCR markers for the GmAHAS4 P180S mutants, which can easily discriminate homozygous, heterozygous mutants, and wild-type plants. This study demonstrates a feasible and effective way to generate herbicide-resistant soybean by using CRISPR/Cas9-mediated base editing. MDPI 2023-05-19 /pmc/articles/PMC10215408/ /pubmed/37237553 http://dx.doi.org/10.3390/biology12050741 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Wei, Tao Jiang, Linjian You, Xiang Ma, Pengyu Xi, Zhen Wang, Ning Ning Generation of Herbicide-Resistant Soybean by Base Editing |
title | Generation of Herbicide-Resistant Soybean by Base Editing |
title_full | Generation of Herbicide-Resistant Soybean by Base Editing |
title_fullStr | Generation of Herbicide-Resistant Soybean by Base Editing |
title_full_unstemmed | Generation of Herbicide-Resistant Soybean by Base Editing |
title_short | Generation of Herbicide-Resistant Soybean by Base Editing |
title_sort | generation of herbicide-resistant soybean by base editing |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215408/ https://www.ncbi.nlm.nih.gov/pubmed/37237553 http://dx.doi.org/10.3390/biology12050741 |
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