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Development of PCL PolyHIPE Substrates for 3D Breast Cancer Cell Culture

Cancer is a becoming a huge social and economic burden on society, becoming one of the most significant barriers to life expectancy in the 21st century. In particular, breast cancer is one of the leading causes of death for women. One of the most significant difficulties to finding efficient therapi...

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Autores principales: Jackson, Caitlin E., Ramos-Rodriguez, David H., Farr, Nicholas T. H., English, William R., Green, Nicola H., Claeyssens, Frederik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215449/
https://www.ncbi.nlm.nih.gov/pubmed/37237592
http://dx.doi.org/10.3390/bioengineering10050522
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author Jackson, Caitlin E.
Ramos-Rodriguez, David H.
Farr, Nicholas T. H.
English, William R.
Green, Nicola H.
Claeyssens, Frederik
author_facet Jackson, Caitlin E.
Ramos-Rodriguez, David H.
Farr, Nicholas T. H.
English, William R.
Green, Nicola H.
Claeyssens, Frederik
author_sort Jackson, Caitlin E.
collection PubMed
description Cancer is a becoming a huge social and economic burden on society, becoming one of the most significant barriers to life expectancy in the 21st century. In particular, breast cancer is one of the leading causes of death for women. One of the most significant difficulties to finding efficient therapies for specific cancers, such as breast cancer, is the efficiency and ease of drug development and testing. Tissue-engineered (TE) in vitro models are rapidly developing as an alternative to animal testing for pharmaceuticals. Additionally, porosity included within these structures overcomes the diffusional mass transfer limit whilst enabling cell infiltration and integration with surrounding tissue. Within this study, we investigated the use of high-molecular-weight polycaprolactone methacrylate (PCL–M) polymerised high-internal-phase emulsions (polyHIPEs) as a scaffold to support 3D breast cancer (MDA-MB-231) cell culture. We assessed the porosity, interconnectivity, and morphology of the polyHIPEs when varying mixing speed during formation of the emulsion, successfully demonstrating the tunability of these polyHIPEs. An ex ovo chick chorioallantoic membrane assay identified the scaffolds as bioinert, with biocompatible properties within a vascularised tissue. Furthermore, in vitro assessment of cell attachment and proliferation showed promising potential for the use of PCL polyHIPEs to support cell growth. Our results demonstrate that PCL polyHIPEs are a promising material to support cancer cell growth with tuneable porosity and interconnectivity for the fabrication of perfusable 3D cancer models.
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spelling pubmed-102154492023-05-27 Development of PCL PolyHIPE Substrates for 3D Breast Cancer Cell Culture Jackson, Caitlin E. Ramos-Rodriguez, David H. Farr, Nicholas T. H. English, William R. Green, Nicola H. Claeyssens, Frederik Bioengineering (Basel) Article Cancer is a becoming a huge social and economic burden on society, becoming one of the most significant barriers to life expectancy in the 21st century. In particular, breast cancer is one of the leading causes of death for women. One of the most significant difficulties to finding efficient therapies for specific cancers, such as breast cancer, is the efficiency and ease of drug development and testing. Tissue-engineered (TE) in vitro models are rapidly developing as an alternative to animal testing for pharmaceuticals. Additionally, porosity included within these structures overcomes the diffusional mass transfer limit whilst enabling cell infiltration and integration with surrounding tissue. Within this study, we investigated the use of high-molecular-weight polycaprolactone methacrylate (PCL–M) polymerised high-internal-phase emulsions (polyHIPEs) as a scaffold to support 3D breast cancer (MDA-MB-231) cell culture. We assessed the porosity, interconnectivity, and morphology of the polyHIPEs when varying mixing speed during formation of the emulsion, successfully demonstrating the tunability of these polyHIPEs. An ex ovo chick chorioallantoic membrane assay identified the scaffolds as bioinert, with biocompatible properties within a vascularised tissue. Furthermore, in vitro assessment of cell attachment and proliferation showed promising potential for the use of PCL polyHIPEs to support cell growth. Our results demonstrate that PCL polyHIPEs are a promising material to support cancer cell growth with tuneable porosity and interconnectivity for the fabrication of perfusable 3D cancer models. MDPI 2023-04-26 /pmc/articles/PMC10215449/ /pubmed/37237592 http://dx.doi.org/10.3390/bioengineering10050522 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jackson, Caitlin E.
Ramos-Rodriguez, David H.
Farr, Nicholas T. H.
English, William R.
Green, Nicola H.
Claeyssens, Frederik
Development of PCL PolyHIPE Substrates for 3D Breast Cancer Cell Culture
title Development of PCL PolyHIPE Substrates for 3D Breast Cancer Cell Culture
title_full Development of PCL PolyHIPE Substrates for 3D Breast Cancer Cell Culture
title_fullStr Development of PCL PolyHIPE Substrates for 3D Breast Cancer Cell Culture
title_full_unstemmed Development of PCL PolyHIPE Substrates for 3D Breast Cancer Cell Culture
title_short Development of PCL PolyHIPE Substrates for 3D Breast Cancer Cell Culture
title_sort development of pcl polyhipe substrates for 3d breast cancer cell culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215449/
https://www.ncbi.nlm.nih.gov/pubmed/37237592
http://dx.doi.org/10.3390/bioengineering10050522
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