Cargando…
Influence of Grafting on Rootstock Rhizosphere Microbiome Assembly in Rosa sp. ‘Natal Brier’
SIMPLE SUMMARY: Microorganisms are tightly associated with crops and can be pathogens or beneficials. Rose is the main ornamental crop worldwide. During production, rose varieties (over 100 in a single farm) are permanently grafted in a unique genotype as rootstock to improve plant performance. This...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215458/ https://www.ncbi.nlm.nih.gov/pubmed/37237477 http://dx.doi.org/10.3390/biology12050663 |
_version_ | 1785048067814195200 |
---|---|
author | Ramirez-Villacis, Dario X. Erazo-Garcia, Pablo Quijia-Pillajo, Juan Llerena-Llerena, Sol Barriga-Medina, Noelia Jones, Corbin D. Leon-Reyes, Antonio |
author_facet | Ramirez-Villacis, Dario X. Erazo-Garcia, Pablo Quijia-Pillajo, Juan Llerena-Llerena, Sol Barriga-Medina, Noelia Jones, Corbin D. Leon-Reyes, Antonio |
author_sort | Ramirez-Villacis, Dario X. |
collection | PubMed |
description | SIMPLE SUMMARY: Microorganisms are tightly associated with crops and can be pathogens or beneficials. Rose is the main ornamental crop worldwide. During production, rose varieties (over 100 in a single farm) are permanently grafted in a unique genotype as rootstock to improve plant performance. This work aimed to study the effect of grafting on root-associated microorganisms using next-generation DNA sequencing analysis. To this end, we have demonstrated that root-associated microorganisms of the rootstock (bacteria and fungi) will depend on the grafted genotype. In other words, a change in the variety will drive unique changes in the assembly of microorganisms at the root. This manuscript discusses differences in the bacterial and fungal communities when grafted and non-grafted and their potential impact on plant performance and agriculture. ABSTRACT: The root microbiome is vital in plant development and health and is highly influenced by crop cultural practices. Rose (Rosa sp.) is the most popular cut flower worldwide. Grafting in rose production is a standard practice to increase yield, improve flower quality, or reduce root-associated pests and diseases. ‘Natal Brier’ is a standard rootstock used in most commercial operations in Ecuador and Colombia, leading countries in producing and exporting ornamentals. It is known that the rose scion genotype affects root biomass and the root exudate profile of grafted plants. However, little is known about the influence of the rose scion genotype on the rhizosphere microbiome. We examined the influence of grafting and scion genotype on the rhizosphere microbiome of the rootstock ‘Natal Brier’. The microbiomes of the non-grafted rootstock and the rootstock grafted with two red rose cultivars were assessed using 16S rRNA and ITS sequencing. Grafting changed microbial community structure and function. Further, analysis of grafted plant samples revealed that the scion genotype highly influences the rootstock microbiome. Under the presented experimental conditions, the rootstock ‘Natal Brier’ core microbiome consisted of 16 bacterial and 40 fungal taxa. Our results highlight that the scion genotype influences root microbe’s recruitment, which might also influence the functionality of assembled microbiomes. |
format | Online Article Text |
id | pubmed-10215458 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102154582023-05-27 Influence of Grafting on Rootstock Rhizosphere Microbiome Assembly in Rosa sp. ‘Natal Brier’ Ramirez-Villacis, Dario X. Erazo-Garcia, Pablo Quijia-Pillajo, Juan Llerena-Llerena, Sol Barriga-Medina, Noelia Jones, Corbin D. Leon-Reyes, Antonio Biology (Basel) Article SIMPLE SUMMARY: Microorganisms are tightly associated with crops and can be pathogens or beneficials. Rose is the main ornamental crop worldwide. During production, rose varieties (over 100 in a single farm) are permanently grafted in a unique genotype as rootstock to improve plant performance. This work aimed to study the effect of grafting on root-associated microorganisms using next-generation DNA sequencing analysis. To this end, we have demonstrated that root-associated microorganisms of the rootstock (bacteria and fungi) will depend on the grafted genotype. In other words, a change in the variety will drive unique changes in the assembly of microorganisms at the root. This manuscript discusses differences in the bacterial and fungal communities when grafted and non-grafted and their potential impact on plant performance and agriculture. ABSTRACT: The root microbiome is vital in plant development and health and is highly influenced by crop cultural practices. Rose (Rosa sp.) is the most popular cut flower worldwide. Grafting in rose production is a standard practice to increase yield, improve flower quality, or reduce root-associated pests and diseases. ‘Natal Brier’ is a standard rootstock used in most commercial operations in Ecuador and Colombia, leading countries in producing and exporting ornamentals. It is known that the rose scion genotype affects root biomass and the root exudate profile of grafted plants. However, little is known about the influence of the rose scion genotype on the rhizosphere microbiome. We examined the influence of grafting and scion genotype on the rhizosphere microbiome of the rootstock ‘Natal Brier’. The microbiomes of the non-grafted rootstock and the rootstock grafted with two red rose cultivars were assessed using 16S rRNA and ITS sequencing. Grafting changed microbial community structure and function. Further, analysis of grafted plant samples revealed that the scion genotype highly influences the rootstock microbiome. Under the presented experimental conditions, the rootstock ‘Natal Brier’ core microbiome consisted of 16 bacterial and 40 fungal taxa. Our results highlight that the scion genotype influences root microbe’s recruitment, which might also influence the functionality of assembled microbiomes. MDPI 2023-04-27 /pmc/articles/PMC10215458/ /pubmed/37237477 http://dx.doi.org/10.3390/biology12050663 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ramirez-Villacis, Dario X. Erazo-Garcia, Pablo Quijia-Pillajo, Juan Llerena-Llerena, Sol Barriga-Medina, Noelia Jones, Corbin D. Leon-Reyes, Antonio Influence of Grafting on Rootstock Rhizosphere Microbiome Assembly in Rosa sp. ‘Natal Brier’ |
title | Influence of Grafting on Rootstock Rhizosphere Microbiome Assembly in Rosa sp. ‘Natal Brier’ |
title_full | Influence of Grafting on Rootstock Rhizosphere Microbiome Assembly in Rosa sp. ‘Natal Brier’ |
title_fullStr | Influence of Grafting on Rootstock Rhizosphere Microbiome Assembly in Rosa sp. ‘Natal Brier’ |
title_full_unstemmed | Influence of Grafting on Rootstock Rhizosphere Microbiome Assembly in Rosa sp. ‘Natal Brier’ |
title_short | Influence of Grafting on Rootstock Rhizosphere Microbiome Assembly in Rosa sp. ‘Natal Brier’ |
title_sort | influence of grafting on rootstock rhizosphere microbiome assembly in rosa sp. ‘natal brier’ |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215458/ https://www.ncbi.nlm.nih.gov/pubmed/37237477 http://dx.doi.org/10.3390/biology12050663 |
work_keys_str_mv | AT ramirezvillacisdariox influenceofgraftingonrootstockrhizospheremicrobiomeassemblyinrosaspnatalbrier AT erazogarciapablo influenceofgraftingonrootstockrhizospheremicrobiomeassemblyinrosaspnatalbrier AT quijiapillajojuan influenceofgraftingonrootstockrhizospheremicrobiomeassemblyinrosaspnatalbrier AT llerenallerenasol influenceofgraftingonrootstockrhizospheremicrobiomeassemblyinrosaspnatalbrier AT barrigamedinanoelia influenceofgraftingonrootstockrhizospheremicrobiomeassemblyinrosaspnatalbrier AT jonescorbind influenceofgraftingonrootstockrhizospheremicrobiomeassemblyinrosaspnatalbrier AT leonreyesantonio influenceofgraftingonrootstockrhizospheremicrobiomeassemblyinrosaspnatalbrier |