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Paclitaxel Protects against Isoproterenol-Induced Damage in Rat Myocardium: Its Heme-Oxygenase Mediated Role in Cardiovascular Research

(1) Background: In cardiovascular applications, paclitaxel inhibits smooth muscle cell proliferation and migration and significantly reduces the occurrence of restenosis and target lesion revascularization. However, the cellular effects of paclitaxel in the myocardium are not well understood; (2) Me...

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Autores principales: Matusovits, Danica, Murlasits, Zsolt, Kupai, Krisztina, Baráth, Zoltán, Kang, Hsu Lin, Osváth, Péter, Szűcs, Miklós, Priksz, Dániel, Juhász, Béla, Radák, Zsolt, Várkonyi, Tamás, Pavo, Imre, Pósa, Anikó
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215731/
https://www.ncbi.nlm.nih.gov/pubmed/37237995
http://dx.doi.org/10.3390/antiox12051129
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author Matusovits, Danica
Murlasits, Zsolt
Kupai, Krisztina
Baráth, Zoltán
Kang, Hsu Lin
Osváth, Péter
Szűcs, Miklós
Priksz, Dániel
Juhász, Béla
Radák, Zsolt
Várkonyi, Tamás
Pavo, Imre
Pósa, Anikó
author_facet Matusovits, Danica
Murlasits, Zsolt
Kupai, Krisztina
Baráth, Zoltán
Kang, Hsu Lin
Osváth, Péter
Szűcs, Miklós
Priksz, Dániel
Juhász, Béla
Radák, Zsolt
Várkonyi, Tamás
Pavo, Imre
Pósa, Anikó
author_sort Matusovits, Danica
collection PubMed
description (1) Background: In cardiovascular applications, paclitaxel inhibits smooth muscle cell proliferation and migration and significantly reduces the occurrence of restenosis and target lesion revascularization. However, the cellular effects of paclitaxel in the myocardium are not well understood; (2) Methods: Wistar rats were divided into four groups: control (CTRL), isoproterenol (ISO) treated (1 mg/kg) and two groups treated with paclitaxel (PAC), which was administrated (10 mg/kg/day) for 5 days by gavage/per os alone or in combination (ISO + PAC) 3 weeks after ISO treatment. Ventricular tissue was harvested 24 h later for measurements of heme oxygenase (HO-1), reduced glutathione (GSH), oxidized glutathione (GSSG), superoxide dismutase (SOD), NF-κB, TNF-α and myeloperoxidase (MPO); (3) Results: HO-1 protein concentration, HO-1 activity, SOD protein concentration and total glutathione significantly decreased in response to ISO treatment. When PAC was administered in conjunction with ISO, HO-1, SOD concentration and total glutathione were not different from control levels. MPO activity, NF-κB concentration and TNF-α protein concentration were significantly increased in the ISO-only group, while the levels of these molecules were restored when PAC was co-administered; (4) Conclusions: Oral administration of PAC can maintain the expression of important antioxidants, anti-inflammatory molecules, HO-1, SOD and GSH, and suppress the production of TNF-α, MPO and NF-κB, which are involved in myocardial damage. The principal component of this cellular defense seems to be the expression of HO-1.
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spelling pubmed-102157312023-05-27 Paclitaxel Protects against Isoproterenol-Induced Damage in Rat Myocardium: Its Heme-Oxygenase Mediated Role in Cardiovascular Research Matusovits, Danica Murlasits, Zsolt Kupai, Krisztina Baráth, Zoltán Kang, Hsu Lin Osváth, Péter Szűcs, Miklós Priksz, Dániel Juhász, Béla Radák, Zsolt Várkonyi, Tamás Pavo, Imre Pósa, Anikó Antioxidants (Basel) Article (1) Background: In cardiovascular applications, paclitaxel inhibits smooth muscle cell proliferation and migration and significantly reduces the occurrence of restenosis and target lesion revascularization. However, the cellular effects of paclitaxel in the myocardium are not well understood; (2) Methods: Wistar rats were divided into four groups: control (CTRL), isoproterenol (ISO) treated (1 mg/kg) and two groups treated with paclitaxel (PAC), which was administrated (10 mg/kg/day) for 5 days by gavage/per os alone or in combination (ISO + PAC) 3 weeks after ISO treatment. Ventricular tissue was harvested 24 h later for measurements of heme oxygenase (HO-1), reduced glutathione (GSH), oxidized glutathione (GSSG), superoxide dismutase (SOD), NF-κB, TNF-α and myeloperoxidase (MPO); (3) Results: HO-1 protein concentration, HO-1 activity, SOD protein concentration and total glutathione significantly decreased in response to ISO treatment. When PAC was administered in conjunction with ISO, HO-1, SOD concentration and total glutathione were not different from control levels. MPO activity, NF-κB concentration and TNF-α protein concentration were significantly increased in the ISO-only group, while the levels of these molecules were restored when PAC was co-administered; (4) Conclusions: Oral administration of PAC can maintain the expression of important antioxidants, anti-inflammatory molecules, HO-1, SOD and GSH, and suppress the production of TNF-α, MPO and NF-κB, which are involved in myocardial damage. The principal component of this cellular defense seems to be the expression of HO-1. MDPI 2023-05-20 /pmc/articles/PMC10215731/ /pubmed/37237995 http://dx.doi.org/10.3390/antiox12051129 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matusovits, Danica
Murlasits, Zsolt
Kupai, Krisztina
Baráth, Zoltán
Kang, Hsu Lin
Osváth, Péter
Szűcs, Miklós
Priksz, Dániel
Juhász, Béla
Radák, Zsolt
Várkonyi, Tamás
Pavo, Imre
Pósa, Anikó
Paclitaxel Protects against Isoproterenol-Induced Damage in Rat Myocardium: Its Heme-Oxygenase Mediated Role in Cardiovascular Research
title Paclitaxel Protects against Isoproterenol-Induced Damage in Rat Myocardium: Its Heme-Oxygenase Mediated Role in Cardiovascular Research
title_full Paclitaxel Protects against Isoproterenol-Induced Damage in Rat Myocardium: Its Heme-Oxygenase Mediated Role in Cardiovascular Research
title_fullStr Paclitaxel Protects against Isoproterenol-Induced Damage in Rat Myocardium: Its Heme-Oxygenase Mediated Role in Cardiovascular Research
title_full_unstemmed Paclitaxel Protects against Isoproterenol-Induced Damage in Rat Myocardium: Its Heme-Oxygenase Mediated Role in Cardiovascular Research
title_short Paclitaxel Protects against Isoproterenol-Induced Damage in Rat Myocardium: Its Heme-Oxygenase Mediated Role in Cardiovascular Research
title_sort paclitaxel protects against isoproterenol-induced damage in rat myocardium: its heme-oxygenase mediated role in cardiovascular research
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215731/
https://www.ncbi.nlm.nih.gov/pubmed/37237995
http://dx.doi.org/10.3390/antiox12051129
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