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Minimizing the variables of voiding spot assay for comparison between laboratories
The voiding spot assay (VSA) is increasingly being adopted as a standard method for assessing mouse urinary function. However, VSA outcomes are highly sensitive to housing environment and procedural parameters. Many variables exist among laboratories, including analytical software, type of daily hou...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215753/ https://www.ncbi.nlm.nih.gov/pubmed/37250709 http://dx.doi.org/10.7717/peerj.15420 |
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author | Luo, Chuang Liu, Juan Yang, Jiali Xie, Xiang Yu, Weiqun Chen, Huan |
author_facet | Luo, Chuang Liu, Juan Yang, Jiali Xie, Xiang Yu, Weiqun Chen, Huan |
author_sort | Luo, Chuang |
collection | PubMed |
description | The voiding spot assay (VSA) is increasingly being adopted as a standard method for assessing mouse urinary function. However, VSA outcomes are highly sensitive to housing environment and procedural parameters. Many variables exist among laboratories, including analytical software, type of daily housing cage, transportation, and the time of the day. Some of these variables, such as the time of VSA and analytical software, have been shown to result in inconsistency and incomparability of data. In this study, we evaluated whether the results of VSA can be compared across laboratories by minimizing these variables. We found that analytical tools between Fiji and MATLAB are in good agreement in the quantification of VSA parameters, especially primary voiding spot (PVS) parameters. Unexpectedly, we found that mice housed in different daily home cages did not alter voiding patterns in a standard VSA cage. Nonetheless, we still recommend acclimation when performing VSA in unfamiliar cages. Notably, mice are highly sensitive to transportation and the time in the morning versus afternoon, which can induce significant changes in voiding patterns. Therefore, a standardized period among laboratories and allowing 2–3 days of rest for mice acclimation after transportation are necessary for VSA. Finally, we performed VSA using identical procedural parameters in two laboratories from two geographical locations to compare the results of VSA and concluded that it is possible to generate limited comparable VSA data, such as PVS volume. |
format | Online Article Text |
id | pubmed-10215753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102157532023-05-27 Minimizing the variables of voiding spot assay for comparison between laboratories Luo, Chuang Liu, Juan Yang, Jiali Xie, Xiang Yu, Weiqun Chen, Huan PeerJ Animal Behavior The voiding spot assay (VSA) is increasingly being adopted as a standard method for assessing mouse urinary function. However, VSA outcomes are highly sensitive to housing environment and procedural parameters. Many variables exist among laboratories, including analytical software, type of daily housing cage, transportation, and the time of the day. Some of these variables, such as the time of VSA and analytical software, have been shown to result in inconsistency and incomparability of data. In this study, we evaluated whether the results of VSA can be compared across laboratories by minimizing these variables. We found that analytical tools between Fiji and MATLAB are in good agreement in the quantification of VSA parameters, especially primary voiding spot (PVS) parameters. Unexpectedly, we found that mice housed in different daily home cages did not alter voiding patterns in a standard VSA cage. Nonetheless, we still recommend acclimation when performing VSA in unfamiliar cages. Notably, mice are highly sensitive to transportation and the time in the morning versus afternoon, which can induce significant changes in voiding patterns. Therefore, a standardized period among laboratories and allowing 2–3 days of rest for mice acclimation after transportation are necessary for VSA. Finally, we performed VSA using identical procedural parameters in two laboratories from two geographical locations to compare the results of VSA and concluded that it is possible to generate limited comparable VSA data, such as PVS volume. PeerJ Inc. 2023-05-23 /pmc/articles/PMC10215753/ /pubmed/37250709 http://dx.doi.org/10.7717/peerj.15420 Text en ©2023 Luo et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Animal Behavior Luo, Chuang Liu, Juan Yang, Jiali Xie, Xiang Yu, Weiqun Chen, Huan Minimizing the variables of voiding spot assay for comparison between laboratories |
title | Minimizing the variables of voiding spot assay for comparison between laboratories |
title_full | Minimizing the variables of voiding spot assay for comparison between laboratories |
title_fullStr | Minimizing the variables of voiding spot assay for comparison between laboratories |
title_full_unstemmed | Minimizing the variables of voiding spot assay for comparison between laboratories |
title_short | Minimizing the variables of voiding spot assay for comparison between laboratories |
title_sort | minimizing the variables of voiding spot assay for comparison between laboratories |
topic | Animal Behavior |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215753/ https://www.ncbi.nlm.nih.gov/pubmed/37250709 http://dx.doi.org/10.7717/peerj.15420 |
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