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Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury

Background: Pathological changes associated with spinal cord injury (SCI) can be observed distant, rostral, or caudal to the epicenter of injury. These remote areas represent important therapeutic targets for post-traumatic spinal cord repair. The present study aimed to investigate the following in...

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Autores principales: Garifulin, Ravil, Davleeva, Maria, Izmailov, Andrei, Fadeev, Filip, Markosyan, Vage, Shevchenko, Roman, Minyazeva, Irina, Minekayev, Tagir, Lavrov, Igor, Islamov, Rustem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215755/
https://www.ncbi.nlm.nih.gov/pubmed/37239001
http://dx.doi.org/10.3390/biomedicines11051331
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author Garifulin, Ravil
Davleeva, Maria
Izmailov, Andrei
Fadeev, Filip
Markosyan, Vage
Shevchenko, Roman
Minyazeva, Irina
Minekayev, Tagir
Lavrov, Igor
Islamov, Rustem
author_facet Garifulin, Ravil
Davleeva, Maria
Izmailov, Andrei
Fadeev, Filip
Markosyan, Vage
Shevchenko, Roman
Minyazeva, Irina
Minekayev, Tagir
Lavrov, Igor
Islamov, Rustem
author_sort Garifulin, Ravil
collection PubMed
description Background: Pathological changes associated with spinal cord injury (SCI) can be observed distant, rostral, or caudal to the epicenter of injury. These remote areas represent important therapeutic targets for post-traumatic spinal cord repair. The present study aimed to investigate the following in relation to SCI: distant changes in the spinal cord, peripheral nerve, and muscles. Methods: The changes in the spinal cord, the tibial nerve, and the hind limb muscles were evaluated in control SCI animals and after intravenous infusion of autologous leucoconcentrate enriched with genes encoding neuroprotective factors (VEGF, GDNF, and NCAM), which previously demonstrated a positive effect on post-traumatic restoration. Results: Two months after thoracic contusion in the treated mini pigs, a positive remodeling of the macro- and microglial cells, expression of PSD95 and Chat in the lumbar spinal cord, and preservation of the number and morphological characteristics of the myelinated fibers in the tibial nerve were observed and were aligned with hind limb motor recovery and reduced soleus muscle atrophy. Conclusion: Here, we show the positive effect of autologous genetically enriched leucoconcentrate-producing recombinant neuroprotective factors on targets distant to the primary lesion site in mini pigs with SCI. These findings open new perspectives for the therapy of SCI.
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spelling pubmed-102157552023-05-27 Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury Garifulin, Ravil Davleeva, Maria Izmailov, Andrei Fadeev, Filip Markosyan, Vage Shevchenko, Roman Minyazeva, Irina Minekayev, Tagir Lavrov, Igor Islamov, Rustem Biomedicines Article Background: Pathological changes associated with spinal cord injury (SCI) can be observed distant, rostral, or caudal to the epicenter of injury. These remote areas represent important therapeutic targets for post-traumatic spinal cord repair. The present study aimed to investigate the following in relation to SCI: distant changes in the spinal cord, peripheral nerve, and muscles. Methods: The changes in the spinal cord, the tibial nerve, and the hind limb muscles were evaluated in control SCI animals and after intravenous infusion of autologous leucoconcentrate enriched with genes encoding neuroprotective factors (VEGF, GDNF, and NCAM), which previously demonstrated a positive effect on post-traumatic restoration. Results: Two months after thoracic contusion in the treated mini pigs, a positive remodeling of the macro- and microglial cells, expression of PSD95 and Chat in the lumbar spinal cord, and preservation of the number and morphological characteristics of the myelinated fibers in the tibial nerve were observed and were aligned with hind limb motor recovery and reduced soleus muscle atrophy. Conclusion: Here, we show the positive effect of autologous genetically enriched leucoconcentrate-producing recombinant neuroprotective factors on targets distant to the primary lesion site in mini pigs with SCI. These findings open new perspectives for the therapy of SCI. MDPI 2023-04-30 /pmc/articles/PMC10215755/ /pubmed/37239001 http://dx.doi.org/10.3390/biomedicines11051331 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Garifulin, Ravil
Davleeva, Maria
Izmailov, Andrei
Fadeev, Filip
Markosyan, Vage
Shevchenko, Roman
Minyazeva, Irina
Minekayev, Tagir
Lavrov, Igor
Islamov, Rustem
Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury
title Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury
title_full Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury
title_fullStr Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury
title_full_unstemmed Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury
title_short Evaluation of the Autologous Genetically Enriched Leucoconcentrate on the Lumbar Spinal Cord Morpho-Functional Recovery in a Mini Pig with Thoracic Spine Contusion Injury
title_sort evaluation of the autologous genetically enriched leucoconcentrate on the lumbar spinal cord morpho-functional recovery in a mini pig with thoracic spine contusion injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215755/
https://www.ncbi.nlm.nih.gov/pubmed/37239001
http://dx.doi.org/10.3390/biomedicines11051331
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