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Carbon Nanodots Attenuate Lipid Peroxidation in the LDL Receptor Knockout Mouse Brain

Abnormal cholesterol metabolism can lead to oxidative stress in the brain. Low-density lipoprotein receptor (LDLr) knockout mice are models for studying altered cholesterol metabolism and oxidative stress onset in the brain. Carbon nanodots are a new class of carbon nanomaterials that possess antiox...

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Autores principales: Erikson, Keith M., El-Khouri, Kristina, Petric, Radmila, Tang, Chenhao, Chen, Jinlan, Vasquez, Delicia Esther Cardenas, Fordahl, Steve C., Jia, Zhenquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215887/
https://www.ncbi.nlm.nih.gov/pubmed/37237947
http://dx.doi.org/10.3390/antiox12051081
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author Erikson, Keith M.
El-Khouri, Kristina
Petric, Radmila
Tang, Chenhao
Chen, Jinlan
Vasquez, Delicia Esther Cardenas
Fordahl, Steve C.
Jia, Zhenquan
author_facet Erikson, Keith M.
El-Khouri, Kristina
Petric, Radmila
Tang, Chenhao
Chen, Jinlan
Vasquez, Delicia Esther Cardenas
Fordahl, Steve C.
Jia, Zhenquan
author_sort Erikson, Keith M.
collection PubMed
description Abnormal cholesterol metabolism can lead to oxidative stress in the brain. Low-density lipoprotein receptor (LDLr) knockout mice are models for studying altered cholesterol metabolism and oxidative stress onset in the brain. Carbon nanodots are a new class of carbon nanomaterials that possess antioxidant properties. The goal of our study was to evaluate the effectiveness of carbon nanodots in preventing brain lipid peroxidation. LDLr knockout mice and wild-type C57BL/6J mice were treated with saline or 2.5 mg/kg bw of carbon nanodots for a 16-week period. Brains were removed and dissected into the cortex, midbrain, and striatum. We measured lipid peroxidation in the mouse brain tissues using the Thiobarbituric Acid Reactive Substances Assay and iron and copper concentrations using Graphite Furnace Atomic Absorption Spectroscopy. We focused on iron and copper due to their association with oxidative stress. Iron concentrations were significantly elevated in the midbrain and striatum of the LDLr knockout mice compared to the C57BL/6J mice, whereas lipid peroxidation was greatest in the midbrain and cortex of the LDLr knockout mice. Treatment with carbon nanodots in the LDLr knockout mice attenuated both the rise in iron and lipid peroxidation, but they had no negative effect in the C57BL/6J mice, indicating the anti-oxidative stress properties of carbon nanodots. We also assessed locomotor and anxiety-like behaviors as functional indicators of lipid peroxidation and found that treatment with carbon nanodots prevented the anxiety-like behaviors displayed by the LDLr knockout mice. Overall, our results show that carbon nanodots are safe and may be an effective nanomaterial for combating the harmful effects caused by lipid peroxidation.
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spelling pubmed-102158872023-05-27 Carbon Nanodots Attenuate Lipid Peroxidation in the LDL Receptor Knockout Mouse Brain Erikson, Keith M. El-Khouri, Kristina Petric, Radmila Tang, Chenhao Chen, Jinlan Vasquez, Delicia Esther Cardenas Fordahl, Steve C. Jia, Zhenquan Antioxidants (Basel) Article Abnormal cholesterol metabolism can lead to oxidative stress in the brain. Low-density lipoprotein receptor (LDLr) knockout mice are models for studying altered cholesterol metabolism and oxidative stress onset in the brain. Carbon nanodots are a new class of carbon nanomaterials that possess antioxidant properties. The goal of our study was to evaluate the effectiveness of carbon nanodots in preventing brain lipid peroxidation. LDLr knockout mice and wild-type C57BL/6J mice were treated with saline or 2.5 mg/kg bw of carbon nanodots for a 16-week period. Brains were removed and dissected into the cortex, midbrain, and striatum. We measured lipid peroxidation in the mouse brain tissues using the Thiobarbituric Acid Reactive Substances Assay and iron and copper concentrations using Graphite Furnace Atomic Absorption Spectroscopy. We focused on iron and copper due to their association with oxidative stress. Iron concentrations were significantly elevated in the midbrain and striatum of the LDLr knockout mice compared to the C57BL/6J mice, whereas lipid peroxidation was greatest in the midbrain and cortex of the LDLr knockout mice. Treatment with carbon nanodots in the LDLr knockout mice attenuated both the rise in iron and lipid peroxidation, but they had no negative effect in the C57BL/6J mice, indicating the anti-oxidative stress properties of carbon nanodots. We also assessed locomotor and anxiety-like behaviors as functional indicators of lipid peroxidation and found that treatment with carbon nanodots prevented the anxiety-like behaviors displayed by the LDLr knockout mice. Overall, our results show that carbon nanodots are safe and may be an effective nanomaterial for combating the harmful effects caused by lipid peroxidation. MDPI 2023-05-11 /pmc/articles/PMC10215887/ /pubmed/37237947 http://dx.doi.org/10.3390/antiox12051081 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Erikson, Keith M.
El-Khouri, Kristina
Petric, Radmila
Tang, Chenhao
Chen, Jinlan
Vasquez, Delicia Esther Cardenas
Fordahl, Steve C.
Jia, Zhenquan
Carbon Nanodots Attenuate Lipid Peroxidation in the LDL Receptor Knockout Mouse Brain
title Carbon Nanodots Attenuate Lipid Peroxidation in the LDL Receptor Knockout Mouse Brain
title_full Carbon Nanodots Attenuate Lipid Peroxidation in the LDL Receptor Knockout Mouse Brain
title_fullStr Carbon Nanodots Attenuate Lipid Peroxidation in the LDL Receptor Knockout Mouse Brain
title_full_unstemmed Carbon Nanodots Attenuate Lipid Peroxidation in the LDL Receptor Knockout Mouse Brain
title_short Carbon Nanodots Attenuate Lipid Peroxidation in the LDL Receptor Knockout Mouse Brain
title_sort carbon nanodots attenuate lipid peroxidation in the ldl receptor knockout mouse brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10215887/
https://www.ncbi.nlm.nih.gov/pubmed/37237947
http://dx.doi.org/10.3390/antiox12051081
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