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What to Do if the qPCR Test for SARS-CoV-2 or Other Pathogen Lacks Endogenous Internal Control? A Simple Test on Housekeeping Genes

Some of the products for the molecular diagnosis of infections do not have an endogenous internal control, and this is necessary to ensure that the result is not a false negative. The aim of the project was to design a simple low-cost RT-qPCR test that can confirm the expression of basic metabolism...

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Detalles Bibliográficos
Autores principales: Kuzan, Aleksandra, Tabakov, Ivo, Madej, Lukasz, Mucha, Anna, Fulawka, Lukasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10216122/
https://www.ncbi.nlm.nih.gov/pubmed/37239008
http://dx.doi.org/10.3390/biomedicines11051337