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A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT(®) Technology for Assessing Total Anti-Oxidative Capacity

Background: Oxidative stress (OS) could cause various COVID-19 complications. Recently, we have developed the Pouvoir AntiOxydant Total (PAOT®) technology for reflecting the total antioxidant capacity (TAC) of biological samples. We aimed to investigate systemic oxidative stress status (OSS) and to...

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Autores principales: Pincemail, Joël, Rousseau, Anne-Françoise, Kaux, Jean-François, Cheramy-Bien, Jean-Paul, Bruyère, Christine, Prick, Jeanine, Stern, David, Kaci, Mouna-Messaouda, Maertens De Noordhout, Benoît, Albert, Adelin, Eubelen, Céline, Goff, Caroline Le, Misset, Benoît, Cavalier, Etienne, Charlier, Corinne, Meziane, Smail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10216138/
https://www.ncbi.nlm.nih.gov/pubmed/37238982
http://dx.doi.org/10.3390/biomedicines11051308
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author Pincemail, Joël
Rousseau, Anne-Françoise
Kaux, Jean-François
Cheramy-Bien, Jean-Paul
Bruyère, Christine
Prick, Jeanine
Stern, David
Kaci, Mouna-Messaouda
Maertens De Noordhout, Benoît
Albert, Adelin
Eubelen, Céline
Goff, Caroline Le
Misset, Benoît
Cavalier, Etienne
Charlier, Corinne
Meziane, Smail
author_facet Pincemail, Joël
Rousseau, Anne-Françoise
Kaux, Jean-François
Cheramy-Bien, Jean-Paul
Bruyère, Christine
Prick, Jeanine
Stern, David
Kaci, Mouna-Messaouda
Maertens De Noordhout, Benoît
Albert, Adelin
Eubelen, Céline
Goff, Caroline Le
Misset, Benoît
Cavalier, Etienne
Charlier, Corinne
Meziane, Smail
author_sort Pincemail, Joël
collection PubMed
description Background: Oxidative stress (OS) could cause various COVID-19 complications. Recently, we have developed the Pouvoir AntiOxydant Total (PAOT®) technology for reflecting the total antioxidant capacity (TAC) of biological samples. We aimed to investigate systemic oxidative stress status (OSS) and to evaluate the utility of PAOT® for assessing TAC during the recovery phase in critical COVID-19 patients in a rehabilitation facility. Materials and Methods: In a total of 12 critical COVID-19 patients in rehabilitation, 19 plasma OSS biomarkers were measured: antioxidants, TAC, trace elements, oxidative damage to lipids, and inflammatory biomarkers. TAC level was measured in plasma, saliva, skin, and urine, using PAOT and expressed as PAOT-Plasma, -Saliva, -Skin, and -Urine scores, respectively. Plasma OSS biomarker levels were compared with levels from previous studies on hospitalized COVID-19 patients and with the reference population. Correlations between four PAOT scores and plasma OSS biomarker levels were analyzed. Results: During the recovery phase, plasma levels in antioxidants (γ-tocopherol, β-carotene, total glutathione, vitamin C and thiol proteins) were significantly lower than reference intervals, whereas total hydroperoxides and myeloperoxidase (a marker of inflammation) were significantly higher. Copper negatively correlated with total hydroperoxides (r = 0.95, p = 0.001). A similar, deeply modified OSS was already observed in COVID-19 patients hospitalized in an intensive care unit. TAC evaluated in saliva, urine, and skin correlated negatively with copper and with plasma total hydroperoxides. To conclude, the systemic OSS, determined using a large number of biomarkers, was always significantly increased in cured COVID-19 patients during their recovery phase. The less costly evaluation of TAC using an electrochemical method could potentially represent a good alternative to the individual analysis of biomarkers linked to pro-oxidants.
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spelling pubmed-102161382023-05-27 A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT(®) Technology for Assessing Total Anti-Oxidative Capacity Pincemail, Joël Rousseau, Anne-Françoise Kaux, Jean-François Cheramy-Bien, Jean-Paul Bruyère, Christine Prick, Jeanine Stern, David Kaci, Mouna-Messaouda Maertens De Noordhout, Benoît Albert, Adelin Eubelen, Céline Goff, Caroline Le Misset, Benoît Cavalier, Etienne Charlier, Corinne Meziane, Smail Biomedicines Article Background: Oxidative stress (OS) could cause various COVID-19 complications. Recently, we have developed the Pouvoir AntiOxydant Total (PAOT®) technology for reflecting the total antioxidant capacity (TAC) of biological samples. We aimed to investigate systemic oxidative stress status (OSS) and to evaluate the utility of PAOT® for assessing TAC during the recovery phase in critical COVID-19 patients in a rehabilitation facility. Materials and Methods: In a total of 12 critical COVID-19 patients in rehabilitation, 19 plasma OSS biomarkers were measured: antioxidants, TAC, trace elements, oxidative damage to lipids, and inflammatory biomarkers. TAC level was measured in plasma, saliva, skin, and urine, using PAOT and expressed as PAOT-Plasma, -Saliva, -Skin, and -Urine scores, respectively. Plasma OSS biomarker levels were compared with levels from previous studies on hospitalized COVID-19 patients and with the reference population. Correlations between four PAOT scores and plasma OSS biomarker levels were analyzed. Results: During the recovery phase, plasma levels in antioxidants (γ-tocopherol, β-carotene, total glutathione, vitamin C and thiol proteins) were significantly lower than reference intervals, whereas total hydroperoxides and myeloperoxidase (a marker of inflammation) were significantly higher. Copper negatively correlated with total hydroperoxides (r = 0.95, p = 0.001). A similar, deeply modified OSS was already observed in COVID-19 patients hospitalized in an intensive care unit. TAC evaluated in saliva, urine, and skin correlated negatively with copper and with plasma total hydroperoxides. To conclude, the systemic OSS, determined using a large number of biomarkers, was always significantly increased in cured COVID-19 patients during their recovery phase. The less costly evaluation of TAC using an electrochemical method could potentially represent a good alternative to the individual analysis of biomarkers linked to pro-oxidants. MDPI 2023-04-28 /pmc/articles/PMC10216138/ /pubmed/37238982 http://dx.doi.org/10.3390/biomedicines11051308 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pincemail, Joël
Rousseau, Anne-Françoise
Kaux, Jean-François
Cheramy-Bien, Jean-Paul
Bruyère, Christine
Prick, Jeanine
Stern, David
Kaci, Mouna-Messaouda
Maertens De Noordhout, Benoît
Albert, Adelin
Eubelen, Céline
Goff, Caroline Le
Misset, Benoît
Cavalier, Etienne
Charlier, Corinne
Meziane, Smail
A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT(®) Technology for Assessing Total Anti-Oxidative Capacity
title A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT(®) Technology for Assessing Total Anti-Oxidative Capacity
title_full A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT(®) Technology for Assessing Total Anti-Oxidative Capacity
title_fullStr A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT(®) Technology for Assessing Total Anti-Oxidative Capacity
title_full_unstemmed A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT(®) Technology for Assessing Total Anti-Oxidative Capacity
title_short A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOT(®) Technology for Assessing Total Anti-Oxidative Capacity
title_sort pilot study on oxidative stress during the recovery phase in critical covid-19 patients in a rehabilitation facility: potential utility of the paot(®) technology for assessing total anti-oxidative capacity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10216138/
https://www.ncbi.nlm.nih.gov/pubmed/37238982
http://dx.doi.org/10.3390/biomedicines11051308
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