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Development of an Efficient FRET-Based Ratiometric Uranium Biosensor
The dispersion of uranium in the environment can pose a problem for the health of humans and other living organisms. It is therefore important to monitor the bioavailable and hence toxic fraction of uranium in the environment, but no efficient measurement methods exist for this. Our study aims to fi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10216315/ https://www.ncbi.nlm.nih.gov/pubmed/37232922 http://dx.doi.org/10.3390/bios13050561 |
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author | Sauge-Merle, Sandrine Recuerda, Morgane Beccia, Maria Rosa Lemaire, David Cherif, Rym Bremond, Nicolas Merola, Fabienne Bousmah, Yasmina Berthomieu, Catherine |
author_facet | Sauge-Merle, Sandrine Recuerda, Morgane Beccia, Maria Rosa Lemaire, David Cherif, Rym Bremond, Nicolas Merola, Fabienne Bousmah, Yasmina Berthomieu, Catherine |
author_sort | Sauge-Merle, Sandrine |
collection | PubMed |
description | The dispersion of uranium in the environment can pose a problem for the health of humans and other living organisms. It is therefore important to monitor the bioavailable and hence toxic fraction of uranium in the environment, but no efficient measurement methods exist for this. Our study aims to fill this gap by developing a genetically encoded FRET-based ratiometric uranium biosensor. This biosensor was constructed by grafting two fluorescent proteins to both ends of calmodulin, a protein that binds four calcium ions. By modifying the metal-binding sites and the fluorescent proteins, several versions of the biosensor were generated and characterized in vitro. The best combination results in a biosensor that is affine and selective for uranium compared to metals such as calcium or other environmental compounds (sodium, magnesium, chlorine). It has a good dynamic range and should be robust to environmental conditions. In addition, its detection limit is below the uranium limit concentration in drinking water defined by the World Health Organization. This genetically encoded biosensor is a promising tool to develop a uranium whole-cell biosensor. This would make it possible to monitor the bioavailable fraction of uranium in the environment, even in calcium-rich waters. |
format | Online Article Text |
id | pubmed-10216315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102163152023-05-27 Development of an Efficient FRET-Based Ratiometric Uranium Biosensor Sauge-Merle, Sandrine Recuerda, Morgane Beccia, Maria Rosa Lemaire, David Cherif, Rym Bremond, Nicolas Merola, Fabienne Bousmah, Yasmina Berthomieu, Catherine Biosensors (Basel) Article The dispersion of uranium in the environment can pose a problem for the health of humans and other living organisms. It is therefore important to monitor the bioavailable and hence toxic fraction of uranium in the environment, but no efficient measurement methods exist for this. Our study aims to fill this gap by developing a genetically encoded FRET-based ratiometric uranium biosensor. This biosensor was constructed by grafting two fluorescent proteins to both ends of calmodulin, a protein that binds four calcium ions. By modifying the metal-binding sites and the fluorescent proteins, several versions of the biosensor were generated and characterized in vitro. The best combination results in a biosensor that is affine and selective for uranium compared to metals such as calcium or other environmental compounds (sodium, magnesium, chlorine). It has a good dynamic range and should be robust to environmental conditions. In addition, its detection limit is below the uranium limit concentration in drinking water defined by the World Health Organization. This genetically encoded biosensor is a promising tool to develop a uranium whole-cell biosensor. This would make it possible to monitor the bioavailable fraction of uranium in the environment, even in calcium-rich waters. MDPI 2023-05-19 /pmc/articles/PMC10216315/ /pubmed/37232922 http://dx.doi.org/10.3390/bios13050561 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sauge-Merle, Sandrine Recuerda, Morgane Beccia, Maria Rosa Lemaire, David Cherif, Rym Bremond, Nicolas Merola, Fabienne Bousmah, Yasmina Berthomieu, Catherine Development of an Efficient FRET-Based Ratiometric Uranium Biosensor |
title | Development of an Efficient FRET-Based Ratiometric Uranium Biosensor |
title_full | Development of an Efficient FRET-Based Ratiometric Uranium Biosensor |
title_fullStr | Development of an Efficient FRET-Based Ratiometric Uranium Biosensor |
title_full_unstemmed | Development of an Efficient FRET-Based Ratiometric Uranium Biosensor |
title_short | Development of an Efficient FRET-Based Ratiometric Uranium Biosensor |
title_sort | development of an efficient fret-based ratiometric uranium biosensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10216315/ https://www.ncbi.nlm.nih.gov/pubmed/37232922 http://dx.doi.org/10.3390/bios13050561 |
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