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COVID-19: A Possible Contribution of the MAPK Pathway

Background: COVID-19 is characterized by an uncontrolled inflammatory response with high pro-inflammatory cytokine production through the activation of intracellular pathways, such as mitogen-activated protein kinase (MAPK). Viruses are able to exploit the MAPK pathway to their advantage; this pathw...

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Autores principales: Cusato, Jessica, Manca, Alessandra, Palermiti, Alice, Mula, Jacopo, Costanzo, Martina, Antonucci, Miriam, Trunfio, Mattia, Corcione, Silvia, Chiara, Francesco, De Vivo, Elisa Delia, Ianniello, Alice, Ferrara, Micol, Di Perri, Giovanni, De Rosa, Francesco Giuseppe, D’Avolio, Antonio, Calcagno, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10216575/
https://www.ncbi.nlm.nih.gov/pubmed/37239131
http://dx.doi.org/10.3390/biomedicines11051459
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author Cusato, Jessica
Manca, Alessandra
Palermiti, Alice
Mula, Jacopo
Costanzo, Martina
Antonucci, Miriam
Trunfio, Mattia
Corcione, Silvia
Chiara, Francesco
De Vivo, Elisa Delia
Ianniello, Alice
Ferrara, Micol
Di Perri, Giovanni
De Rosa, Francesco Giuseppe
D’Avolio, Antonio
Calcagno, Andrea
author_facet Cusato, Jessica
Manca, Alessandra
Palermiti, Alice
Mula, Jacopo
Costanzo, Martina
Antonucci, Miriam
Trunfio, Mattia
Corcione, Silvia
Chiara, Francesco
De Vivo, Elisa Delia
Ianniello, Alice
Ferrara, Micol
Di Perri, Giovanni
De Rosa, Francesco Giuseppe
D’Avolio, Antonio
Calcagno, Andrea
author_sort Cusato, Jessica
collection PubMed
description Background: COVID-19 is characterized by an uncontrolled inflammatory response with high pro-inflammatory cytokine production through the activation of intracellular pathways, such as mitogen-activated protein kinase (MAPK). Viruses are able to exploit the MAPK pathway to their advantage; this pathway relevance to severe COVID-19 is poorly described. The aim of this study was to quantify biomarkers involved in the MAPK pathway and to clarify its possible role in affecting some COVID-19-related clinical features. Methods: H-RAS, C-RAF, MAPK1, MAPK2, and ERK were quantified through ELISA, and genetic polymorphisms were evaluated through real-time PCR. Results: We prospectively recruited 201 individuals (158 positive and 43 negative for SARS-CoV-2): 35 were male, and their median age was 65 years. MAPK-related biomarker levels were increased in SARS-CoV-2-positive participants (n = 89) compared to negative ones (n = 29). Dyspnea was reported by 48%; this symptom was associated with PBMC C-RAF levels in positive participants (p = 0.022) and type of ventilation (p = 0.031). The highest degree of ventilation was used by 8% for invasive ventilation and 41% for continuous positive airway pressure (CPAP). Conclusions: This is the first study that showed a possible contribution of MAPK-related biomarkers in affecting COVID-19 clinical features, and this may be relevant for identifying COVID-19 positive participants at risk of serious complications.
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spelling pubmed-102165752023-05-27 COVID-19: A Possible Contribution of the MAPK Pathway Cusato, Jessica Manca, Alessandra Palermiti, Alice Mula, Jacopo Costanzo, Martina Antonucci, Miriam Trunfio, Mattia Corcione, Silvia Chiara, Francesco De Vivo, Elisa Delia Ianniello, Alice Ferrara, Micol Di Perri, Giovanni De Rosa, Francesco Giuseppe D’Avolio, Antonio Calcagno, Andrea Biomedicines Article Background: COVID-19 is characterized by an uncontrolled inflammatory response with high pro-inflammatory cytokine production through the activation of intracellular pathways, such as mitogen-activated protein kinase (MAPK). Viruses are able to exploit the MAPK pathway to their advantage; this pathway relevance to severe COVID-19 is poorly described. The aim of this study was to quantify biomarkers involved in the MAPK pathway and to clarify its possible role in affecting some COVID-19-related clinical features. Methods: H-RAS, C-RAF, MAPK1, MAPK2, and ERK were quantified through ELISA, and genetic polymorphisms were evaluated through real-time PCR. Results: We prospectively recruited 201 individuals (158 positive and 43 negative for SARS-CoV-2): 35 were male, and their median age was 65 years. MAPK-related biomarker levels were increased in SARS-CoV-2-positive participants (n = 89) compared to negative ones (n = 29). Dyspnea was reported by 48%; this symptom was associated with PBMC C-RAF levels in positive participants (p = 0.022) and type of ventilation (p = 0.031). The highest degree of ventilation was used by 8% for invasive ventilation and 41% for continuous positive airway pressure (CPAP). Conclusions: This is the first study that showed a possible contribution of MAPK-related biomarkers in affecting COVID-19 clinical features, and this may be relevant for identifying COVID-19 positive participants at risk of serious complications. MDPI 2023-05-16 /pmc/articles/PMC10216575/ /pubmed/37239131 http://dx.doi.org/10.3390/biomedicines11051459 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cusato, Jessica
Manca, Alessandra
Palermiti, Alice
Mula, Jacopo
Costanzo, Martina
Antonucci, Miriam
Trunfio, Mattia
Corcione, Silvia
Chiara, Francesco
De Vivo, Elisa Delia
Ianniello, Alice
Ferrara, Micol
Di Perri, Giovanni
De Rosa, Francesco Giuseppe
D’Avolio, Antonio
Calcagno, Andrea
COVID-19: A Possible Contribution of the MAPK Pathway
title COVID-19: A Possible Contribution of the MAPK Pathway
title_full COVID-19: A Possible Contribution of the MAPK Pathway
title_fullStr COVID-19: A Possible Contribution of the MAPK Pathway
title_full_unstemmed COVID-19: A Possible Contribution of the MAPK Pathway
title_short COVID-19: A Possible Contribution of the MAPK Pathway
title_sort covid-19: a possible contribution of the mapk pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10216575/
https://www.ncbi.nlm.nih.gov/pubmed/37239131
http://dx.doi.org/10.3390/biomedicines11051459
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