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On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions

Among the adenylate carriers identified in Arabidopsis thaliana, only the AMP/ATP transporter ADNT1 shows increased expression in roots under waterlogging stress conditions. Here, we investigated the impact of a reduced expression of ADNT1 in A. thaliana plants submitted to waterlogging conditions....

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Autores principales: Neri-Silva, Roberto, Monteiro-Batista, Rita de Cássia, da Fonseca-Pereira, Paula, Nunes, Mateus Dias, Viana-Silva, Ana Luiza, Palhares Ribeiro, Tamara, Pérez-Díaz, Jorge L., Medeiros, David B., Araújo, Wagner L., Fernie, Alisdair R., Nunes-Nesi, Adriano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10216781/
https://www.ncbi.nlm.nih.gov/pubmed/37238601
http://dx.doi.org/10.3390/biom13050731
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author Neri-Silva, Roberto
Monteiro-Batista, Rita de Cássia
da Fonseca-Pereira, Paula
Nunes, Mateus Dias
Viana-Silva, Ana Luiza
Palhares Ribeiro, Tamara
Pérez-Díaz, Jorge L.
Medeiros, David B.
Araújo, Wagner L.
Fernie, Alisdair R.
Nunes-Nesi, Adriano
author_facet Neri-Silva, Roberto
Monteiro-Batista, Rita de Cássia
da Fonseca-Pereira, Paula
Nunes, Mateus Dias
Viana-Silva, Ana Luiza
Palhares Ribeiro, Tamara
Pérez-Díaz, Jorge L.
Medeiros, David B.
Araújo, Wagner L.
Fernie, Alisdair R.
Nunes-Nesi, Adriano
author_sort Neri-Silva, Roberto
collection PubMed
description Among the adenylate carriers identified in Arabidopsis thaliana, only the AMP/ATP transporter ADNT1 shows increased expression in roots under waterlogging stress conditions. Here, we investigated the impact of a reduced expression of ADNT1 in A. thaliana plants submitted to waterlogging conditions. For this purpose, an adnt1 T-DNA mutant and two ADNT1 antisense lines were evaluated. Following waterlogging, ADNT1 deficiency resulted in a reduced maximum quantum yield of PSII electron transport (significantly for adnt1 and antisense Line 10), indicating a higher impact caused by the stress in the mutants. In addition, ADNT1 deficient lines showed higher levels of AMP in roots under nonstress condition. This result indicates that the downregulation of ADNT1 impacts the levels of adenylates. ADNT1-deficient plants exhibited a differential expression pattern of hypoxia-related genes with an increase in non-fermenting-related-kinase 1 (SnRK1) expression and upregulation of adenylate kinase (ADK) under stress and non-stress conditions. Together, these results indicated that the lower expression of ADNT1 is associated with an early “hypoxic status” due to the perturbation of the adenylate pool caused by reduced AMP import by mitochondria. This perturbation, which is sensed by SnRK1, results in a metabolic reprogramming associated with early induction of the fermentative pathway in ADNT1 deficient plants.
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spelling pubmed-102167812023-05-27 On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions Neri-Silva, Roberto Monteiro-Batista, Rita de Cássia da Fonseca-Pereira, Paula Nunes, Mateus Dias Viana-Silva, Ana Luiza Palhares Ribeiro, Tamara Pérez-Díaz, Jorge L. Medeiros, David B. Araújo, Wagner L. Fernie, Alisdair R. Nunes-Nesi, Adriano Biomolecules Article Among the adenylate carriers identified in Arabidopsis thaliana, only the AMP/ATP transporter ADNT1 shows increased expression in roots under waterlogging stress conditions. Here, we investigated the impact of a reduced expression of ADNT1 in A. thaliana plants submitted to waterlogging conditions. For this purpose, an adnt1 T-DNA mutant and two ADNT1 antisense lines were evaluated. Following waterlogging, ADNT1 deficiency resulted in a reduced maximum quantum yield of PSII electron transport (significantly for adnt1 and antisense Line 10), indicating a higher impact caused by the stress in the mutants. In addition, ADNT1 deficient lines showed higher levels of AMP in roots under nonstress condition. This result indicates that the downregulation of ADNT1 impacts the levels of adenylates. ADNT1-deficient plants exhibited a differential expression pattern of hypoxia-related genes with an increase in non-fermenting-related-kinase 1 (SnRK1) expression and upregulation of adenylate kinase (ADK) under stress and non-stress conditions. Together, these results indicated that the lower expression of ADNT1 is associated with an early “hypoxic status” due to the perturbation of the adenylate pool caused by reduced AMP import by mitochondria. This perturbation, which is sensed by SnRK1, results in a metabolic reprogramming associated with early induction of the fermentative pathway in ADNT1 deficient plants. MDPI 2023-04-24 /pmc/articles/PMC10216781/ /pubmed/37238601 http://dx.doi.org/10.3390/biom13050731 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Neri-Silva, Roberto
Monteiro-Batista, Rita de Cássia
da Fonseca-Pereira, Paula
Nunes, Mateus Dias
Viana-Silva, Ana Luiza
Palhares Ribeiro, Tamara
Pérez-Díaz, Jorge L.
Medeiros, David B.
Araújo, Wagner L.
Fernie, Alisdair R.
Nunes-Nesi, Adriano
On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions
title On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions
title_full On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions
title_fullStr On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions
title_full_unstemmed On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions
title_short On the Significance of the ADNT1 Carrier in Arabidopsis thaliana under Waterlogging Conditions
title_sort on the significance of the adnt1 carrier in arabidopsis thaliana under waterlogging conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10216781/
https://www.ncbi.nlm.nih.gov/pubmed/37238601
http://dx.doi.org/10.3390/biom13050731
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