A LC-QTOF Method for the Determination of PEGDE Residues in Dermal Fillers

Hyaluronic acid is one of the most important ingredients in dermal fillers, where it is often cross-linked to gain more favorable rheological properties and to improve the implant duration. Poly(ethylene glycol) diglycidyl ether (PEGDE) has been recently introduced as a crosslinker because of its ve...

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Autores principales: Alonci, Giuseppe, Boussard, Anna, Savona, Martina, Cordella, Fabiana, Angelici, Gaetano, Mocchi, Roberto, Sommatis, Sabrina, Monticelli, Damiano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217355/
https://www.ncbi.nlm.nih.gov/pubmed/37233001
http://dx.doi.org/10.3390/gels9050409
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author Alonci, Giuseppe
Boussard, Anna
Savona, Martina
Cordella, Fabiana
Angelici, Gaetano
Mocchi, Roberto
Sommatis, Sabrina
Monticelli, Damiano
author_facet Alonci, Giuseppe
Boussard, Anna
Savona, Martina
Cordella, Fabiana
Angelici, Gaetano
Mocchi, Roberto
Sommatis, Sabrina
Monticelli, Damiano
author_sort Alonci, Giuseppe
collection PubMed
description Hyaluronic acid is one of the most important ingredients in dermal fillers, where it is often cross-linked to gain more favorable rheological properties and to improve the implant duration. Poly(ethylene glycol) diglycidyl ether (PEGDE) has been recently introduced as a crosslinker because of its very similar chemical reactivity with the most-used crosslinker BDDE, while giving special rheological properties. Monitoring the amount of the crosslinker residues in the final device is always necessary, but in the case of PEGDE, no methods are available in literature. Here, we present an HPLC-QTOF method, validated according to the guidelines of the International Council on Harmonization, which enables the efficient routine examination of the PEGDE content in HA hydrogels.
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spelling pubmed-102173552023-05-27 A LC-QTOF Method for the Determination of PEGDE Residues in Dermal Fillers Alonci, Giuseppe Boussard, Anna Savona, Martina Cordella, Fabiana Angelici, Gaetano Mocchi, Roberto Sommatis, Sabrina Monticelli, Damiano Gels Protocol Hyaluronic acid is one of the most important ingredients in dermal fillers, where it is often cross-linked to gain more favorable rheological properties and to improve the implant duration. Poly(ethylene glycol) diglycidyl ether (PEGDE) has been recently introduced as a crosslinker because of its very similar chemical reactivity with the most-used crosslinker BDDE, while giving special rheological properties. Monitoring the amount of the crosslinker residues in the final device is always necessary, but in the case of PEGDE, no methods are available in literature. Here, we present an HPLC-QTOF method, validated according to the guidelines of the International Council on Harmonization, which enables the efficient routine examination of the PEGDE content in HA hydrogels. MDPI 2023-05-13 /pmc/articles/PMC10217355/ /pubmed/37233001 http://dx.doi.org/10.3390/gels9050409 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Alonci, Giuseppe
Boussard, Anna
Savona, Martina
Cordella, Fabiana
Angelici, Gaetano
Mocchi, Roberto
Sommatis, Sabrina
Monticelli, Damiano
A LC-QTOF Method for the Determination of PEGDE Residues in Dermal Fillers
title A LC-QTOF Method for the Determination of PEGDE Residues in Dermal Fillers
title_full A LC-QTOF Method for the Determination of PEGDE Residues in Dermal Fillers
title_fullStr A LC-QTOF Method for the Determination of PEGDE Residues in Dermal Fillers
title_full_unstemmed A LC-QTOF Method for the Determination of PEGDE Residues in Dermal Fillers
title_short A LC-QTOF Method for the Determination of PEGDE Residues in Dermal Fillers
title_sort lc-qtof method for the determination of pegde residues in dermal fillers
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217355/
https://www.ncbi.nlm.nih.gov/pubmed/37233001
http://dx.doi.org/10.3390/gels9050409
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