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Increase in gut permeability and oxidized ldl is associated with post-acute sequelae of SARS-CoV-2

BACKGROUND: Post-acute sequelae of SARS-CoV-2 (PASC) is marked by persistent or newly developing symptoms beyond 4 weeks of infection. Investigating gut integrity, oxidized lipids and inflammatory markers is important for understanding PASC pathogenesis. METHODS: A cross-sectional study including CO...

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Autores principales: Mouchati, Christian, Durieux, Jared C., Zisis, Sokratis N., Labbato, Danielle, Rodgers, Michael A., Ailstock, Kate, Reinert, Brian L., Funderburg, Nicholas T., McComsey, Grace A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217362/
https://www.ncbi.nlm.nih.gov/pubmed/37251403
http://dx.doi.org/10.3389/fimmu.2023.1182544
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author Mouchati, Christian
Durieux, Jared C.
Zisis, Sokratis N.
Labbato, Danielle
Rodgers, Michael A.
Ailstock, Kate
Reinert, Brian L.
Funderburg, Nicholas T.
McComsey, Grace A.
author_facet Mouchati, Christian
Durieux, Jared C.
Zisis, Sokratis N.
Labbato, Danielle
Rodgers, Michael A.
Ailstock, Kate
Reinert, Brian L.
Funderburg, Nicholas T.
McComsey, Grace A.
author_sort Mouchati, Christian
collection PubMed
description BACKGROUND: Post-acute sequelae of SARS-CoV-2 (PASC) is marked by persistent or newly developing symptoms beyond 4 weeks of infection. Investigating gut integrity, oxidized lipids and inflammatory markers is important for understanding PASC pathogenesis. METHODS: A cross-sectional study including COVID+ with PASC, COVID+ without PASC, and COVID-negative (COVID-) participants. We measured plasma markers by enzyme-linked immunosorbent assay to assess intestinal permeability (ZONULIN), microbial translocation (lipopolysaccharide-binding protein or LBP), systemic inflammation (high-sensitivity C-reactive protein or hs-CRP), and oxidized low-density lipoprotein (Ox-LDL). RESULTS: 415 participants were enrolled in this study; 37.83% (n=157) had prior COVID diagnosis and among COVID+, 54% (n=85) had PASC. The median zonulin among COVID- was 3.37 (IQR: 2.13, 4.91) mg/mL, 3.43 (IQR: 1.65, 5.25) mg/mL among COVID+ no PASC, and highest [4.76 (IQR: 3.2, 7.35) mg/mL] among COVID+ PASC+ (p<.0001). The median ox-LDL among COVID- was 47.02 (IQR: 35.52, 62.77) U/L, 57.24 (IQR: 40.7, 75.37) U/L among COVID+ No PASC, and the highest [76.75 (IQR: 59.95, 103.28) U/L] among COVID+ PASC+ (p<.0001). COVID+ PASC+ was positively associated with zonulin (p=0.0002) and ox-LDL (p<.0001), and COVID- was negatively associated with ox-LDL (p=0.01), compared to COVID+ No PASC. Every unit increase in zonulin was associated with 44% higher predicted odds of having PASC [aOR: 1.44 (95%CI: 1.1, 1.9)] and every one-unit increase in ox-LDL was associated with more than four-fold increased odds of having PASC [aOR: 2.44 (95%CI: 1.67, 3.55)]. CONCLUSIONS: PASC is associated with increased gut permeability and oxidized lipids. Further studies are needed to clarify whether these relationships are causal which could lead to targeted therapeutics.
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spelling pubmed-102173622023-05-27 Increase in gut permeability and oxidized ldl is associated with post-acute sequelae of SARS-CoV-2 Mouchati, Christian Durieux, Jared C. Zisis, Sokratis N. Labbato, Danielle Rodgers, Michael A. Ailstock, Kate Reinert, Brian L. Funderburg, Nicholas T. McComsey, Grace A. Front Immunol Immunology BACKGROUND: Post-acute sequelae of SARS-CoV-2 (PASC) is marked by persistent or newly developing symptoms beyond 4 weeks of infection. Investigating gut integrity, oxidized lipids and inflammatory markers is important for understanding PASC pathogenesis. METHODS: A cross-sectional study including COVID+ with PASC, COVID+ without PASC, and COVID-negative (COVID-) participants. We measured plasma markers by enzyme-linked immunosorbent assay to assess intestinal permeability (ZONULIN), microbial translocation (lipopolysaccharide-binding protein or LBP), systemic inflammation (high-sensitivity C-reactive protein or hs-CRP), and oxidized low-density lipoprotein (Ox-LDL). RESULTS: 415 participants were enrolled in this study; 37.83% (n=157) had prior COVID diagnosis and among COVID+, 54% (n=85) had PASC. The median zonulin among COVID- was 3.37 (IQR: 2.13, 4.91) mg/mL, 3.43 (IQR: 1.65, 5.25) mg/mL among COVID+ no PASC, and highest [4.76 (IQR: 3.2, 7.35) mg/mL] among COVID+ PASC+ (p<.0001). The median ox-LDL among COVID- was 47.02 (IQR: 35.52, 62.77) U/L, 57.24 (IQR: 40.7, 75.37) U/L among COVID+ No PASC, and the highest [76.75 (IQR: 59.95, 103.28) U/L] among COVID+ PASC+ (p<.0001). COVID+ PASC+ was positively associated with zonulin (p=0.0002) and ox-LDL (p<.0001), and COVID- was negatively associated with ox-LDL (p=0.01), compared to COVID+ No PASC. Every unit increase in zonulin was associated with 44% higher predicted odds of having PASC [aOR: 1.44 (95%CI: 1.1, 1.9)] and every one-unit increase in ox-LDL was associated with more than four-fold increased odds of having PASC [aOR: 2.44 (95%CI: 1.67, 3.55)]. CONCLUSIONS: PASC is associated with increased gut permeability and oxidized lipids. Further studies are needed to clarify whether these relationships are causal which could lead to targeted therapeutics. Frontiers Media S.A. 2023-05-12 /pmc/articles/PMC10217362/ /pubmed/37251403 http://dx.doi.org/10.3389/fimmu.2023.1182544 Text en Copyright © 2023 Mouchati, Durieux, Zisis, Labbato, Rodgers, Ailstock, Reinert, Funderburg and McComsey https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Mouchati, Christian
Durieux, Jared C.
Zisis, Sokratis N.
Labbato, Danielle
Rodgers, Michael A.
Ailstock, Kate
Reinert, Brian L.
Funderburg, Nicholas T.
McComsey, Grace A.
Increase in gut permeability and oxidized ldl is associated with post-acute sequelae of SARS-CoV-2
title Increase in gut permeability and oxidized ldl is associated with post-acute sequelae of SARS-CoV-2
title_full Increase in gut permeability and oxidized ldl is associated with post-acute sequelae of SARS-CoV-2
title_fullStr Increase in gut permeability and oxidized ldl is associated with post-acute sequelae of SARS-CoV-2
title_full_unstemmed Increase in gut permeability and oxidized ldl is associated with post-acute sequelae of SARS-CoV-2
title_short Increase in gut permeability and oxidized ldl is associated with post-acute sequelae of SARS-CoV-2
title_sort increase in gut permeability and oxidized ldl is associated with post-acute sequelae of sars-cov-2
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217362/
https://www.ncbi.nlm.nih.gov/pubmed/37251403
http://dx.doi.org/10.3389/fimmu.2023.1182544
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