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SARS-CoV-2 and Swabs: Disease Severity and the Numbers of Cycles of Gene Amplification, Single Center Experience

Pediatric COVID-19 determines a mild clinical picture, but few data have been published about the correlation between disease severity and PCR amplification cycles of SARS-CoV-2 from respiratory samples. This correlation is clinically important because it permits the stratification of patients in re...

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Autores principales: Falsaperla, Raffaele, Sortino, Vincenzo, Collotta, Ausilia Desiree, Marino, Silvia, Pavone, Piero, Grassi, Laura, Privitera, Grete Francesca, Ruggieri, Martino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217405/
https://www.ncbi.nlm.nih.gov/pubmed/37238389
http://dx.doi.org/10.3390/children10050841
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author Falsaperla, Raffaele
Sortino, Vincenzo
Collotta, Ausilia Desiree
Marino, Silvia
Pavone, Piero
Grassi, Laura
Privitera, Grete Francesca
Ruggieri, Martino
author_facet Falsaperla, Raffaele
Sortino, Vincenzo
Collotta, Ausilia Desiree
Marino, Silvia
Pavone, Piero
Grassi, Laura
Privitera, Grete Francesca
Ruggieri, Martino
author_sort Falsaperla, Raffaele
collection PubMed
description Pediatric COVID-19 determines a mild clinical picture, but few data have been published about the correlation between disease severity and PCR amplification cycles of SARS-CoV-2 from respiratory samples. This correlation is clinically important because it permits the stratification of patients in relation to their risk of developing a serious disease. Therefore, the primary endpoint of this study was to establish whether disease severity at the onset, when evaluated with a LqSOFA score, correlated with the gene amplification of SARS-CoV-2. LqSOFA score, also named the Liverpool quick Sequential Organ Failure Assessment, is a pediatric score that indicates the severity of illness with a range from 0 to 4 that incorporates age-adjusted heart rate, respiratory rate, capillary refill and consciousness level (AVPU). The secondary endpoint was to determine if this score could predict the days of duration for symptoms and positive swabs. Our study included 124 patients aged between 0 and 18 years. The LqSOFA score was negatively correlated with the number of PCR amplification cycles, but this was not significant (Pearson’s index −0.14, p-value 0.13). Instead, the correlation between the LqSOFA score and the duration of symptoms was positively related and statistically significant (Pearson’s index 0.20, p-value 0.02), such as the correlation between the LqSOFA score and the duration of a positive swab (Pearson’s index 0.40, p-value < 0.01). So, the LqSOFA score upon admission may predict the duration of symptoms and positive swabs; the PCR amplification of SARS-CoV-2 appears not to play a key role at onset in the prediction of disease severity.
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spelling pubmed-102174052023-05-27 SARS-CoV-2 and Swabs: Disease Severity and the Numbers of Cycles of Gene Amplification, Single Center Experience Falsaperla, Raffaele Sortino, Vincenzo Collotta, Ausilia Desiree Marino, Silvia Pavone, Piero Grassi, Laura Privitera, Grete Francesca Ruggieri, Martino Children (Basel) Article Pediatric COVID-19 determines a mild clinical picture, but few data have been published about the correlation between disease severity and PCR amplification cycles of SARS-CoV-2 from respiratory samples. This correlation is clinically important because it permits the stratification of patients in relation to their risk of developing a serious disease. Therefore, the primary endpoint of this study was to establish whether disease severity at the onset, when evaluated with a LqSOFA score, correlated with the gene amplification of SARS-CoV-2. LqSOFA score, also named the Liverpool quick Sequential Organ Failure Assessment, is a pediatric score that indicates the severity of illness with a range from 0 to 4 that incorporates age-adjusted heart rate, respiratory rate, capillary refill and consciousness level (AVPU). The secondary endpoint was to determine if this score could predict the days of duration for symptoms and positive swabs. Our study included 124 patients aged between 0 and 18 years. The LqSOFA score was negatively correlated with the number of PCR amplification cycles, but this was not significant (Pearson’s index −0.14, p-value 0.13). Instead, the correlation between the LqSOFA score and the duration of symptoms was positively related and statistically significant (Pearson’s index 0.20, p-value 0.02), such as the correlation between the LqSOFA score and the duration of a positive swab (Pearson’s index 0.40, p-value < 0.01). So, the LqSOFA score upon admission may predict the duration of symptoms and positive swabs; the PCR amplification of SARS-CoV-2 appears not to play a key role at onset in the prediction of disease severity. MDPI 2023-05-06 /pmc/articles/PMC10217405/ /pubmed/37238389 http://dx.doi.org/10.3390/children10050841 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Falsaperla, Raffaele
Sortino, Vincenzo
Collotta, Ausilia Desiree
Marino, Silvia
Pavone, Piero
Grassi, Laura
Privitera, Grete Francesca
Ruggieri, Martino
SARS-CoV-2 and Swabs: Disease Severity and the Numbers of Cycles of Gene Amplification, Single Center Experience
title SARS-CoV-2 and Swabs: Disease Severity and the Numbers of Cycles of Gene Amplification, Single Center Experience
title_full SARS-CoV-2 and Swabs: Disease Severity and the Numbers of Cycles of Gene Amplification, Single Center Experience
title_fullStr SARS-CoV-2 and Swabs: Disease Severity and the Numbers of Cycles of Gene Amplification, Single Center Experience
title_full_unstemmed SARS-CoV-2 and Swabs: Disease Severity and the Numbers of Cycles of Gene Amplification, Single Center Experience
title_short SARS-CoV-2 and Swabs: Disease Severity and the Numbers of Cycles of Gene Amplification, Single Center Experience
title_sort sars-cov-2 and swabs: disease severity and the numbers of cycles of gene amplification, single center experience
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217405/
https://www.ncbi.nlm.nih.gov/pubmed/37238389
http://dx.doi.org/10.3390/children10050841
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