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High TLR6 Expression Status Predicts a More Favorable Prognosis after Esophagectomy for Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma

Most so-called “beneficial bacteria” in gut microbiota are Gram-positive, and TLR6 recognizes the peptidoglycan (PGN) present in their cell walls. We hypothesized that a high TLR6 expression status predicts a more favorable prognosis after esophagectomy. We used an ESCC tissue microarray (TMA) to ex...

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Autores principales: Sato, Yusuke, Wakita, Akiyuki, Maeda, Eri, Nagaki, Yushi, Sasamori, Ryohei, Kemuriyama, Kohei, Nozaki, Shu, Ito, Satoru, Terata, Kaori, Imai, Kazuhiro, Nanjo, Hiroshi, Nomura, Kyoko, Minamiya, Yoshihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217459/
https://www.ncbi.nlm.nih.gov/pubmed/37232814
http://dx.doi.org/10.3390/curroncol30050356
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author Sato, Yusuke
Wakita, Akiyuki
Maeda, Eri
Nagaki, Yushi
Sasamori, Ryohei
Kemuriyama, Kohei
Nozaki, Shu
Ito, Satoru
Terata, Kaori
Imai, Kazuhiro
Nanjo, Hiroshi
Nomura, Kyoko
Minamiya, Yoshihiro
author_facet Sato, Yusuke
Wakita, Akiyuki
Maeda, Eri
Nagaki, Yushi
Sasamori, Ryohei
Kemuriyama, Kohei
Nozaki, Shu
Ito, Satoru
Terata, Kaori
Imai, Kazuhiro
Nanjo, Hiroshi
Nomura, Kyoko
Minamiya, Yoshihiro
author_sort Sato, Yusuke
collection PubMed
description Most so-called “beneficial bacteria” in gut microbiota are Gram-positive, and TLR6 recognizes the peptidoglycan (PGN) present in their cell walls. We hypothesized that a high TLR6 expression status predicts a more favorable prognosis after esophagectomy. We used an ESCC tissue microarray (TMA) to examine TLR6 expression status in ESCC patients and to determine whether TLR6 expression status correlates with prognosis after curative esophagectomy. We also examined whether PGN influences the cell proliferation activity of ESCC lines. Clinical ESCC samples from 177 patients tested for the expression of TLR6 were categorized as 3+ (n = 17), 2+ (n = 48), 1+ (n = 68), or 0 (n = 44). High TLR6 expression (3+ and 2+) correlated with significantly more favorable 5-year overall survival (OS) and disease-specific survival (DSS) after esophagectomy than a lower TLR6 expression (1+ and 0). Univariate and multivariate analyses showed that TLR6 expression status is an independent prognostic factor that affects 5-year OS. PGN significantly inhibited the cell proliferation activity of ESCC lines. This is the first study to show that high TLR6 expression status predicts a more favorable prognosis in locally advanced thoracic ESCC patients after curative esophagectomy. PGN released from “beneficial bacteria” seems to have potential to inhibit the cell proliferation activity of ESCC.
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spelling pubmed-102174592023-05-27 High TLR6 Expression Status Predicts a More Favorable Prognosis after Esophagectomy for Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma Sato, Yusuke Wakita, Akiyuki Maeda, Eri Nagaki, Yushi Sasamori, Ryohei Kemuriyama, Kohei Nozaki, Shu Ito, Satoru Terata, Kaori Imai, Kazuhiro Nanjo, Hiroshi Nomura, Kyoko Minamiya, Yoshihiro Curr Oncol Article Most so-called “beneficial bacteria” in gut microbiota are Gram-positive, and TLR6 recognizes the peptidoglycan (PGN) present in their cell walls. We hypothesized that a high TLR6 expression status predicts a more favorable prognosis after esophagectomy. We used an ESCC tissue microarray (TMA) to examine TLR6 expression status in ESCC patients and to determine whether TLR6 expression status correlates with prognosis after curative esophagectomy. We also examined whether PGN influences the cell proliferation activity of ESCC lines. Clinical ESCC samples from 177 patients tested for the expression of TLR6 were categorized as 3+ (n = 17), 2+ (n = 48), 1+ (n = 68), or 0 (n = 44). High TLR6 expression (3+ and 2+) correlated with significantly more favorable 5-year overall survival (OS) and disease-specific survival (DSS) after esophagectomy than a lower TLR6 expression (1+ and 0). Univariate and multivariate analyses showed that TLR6 expression status is an independent prognostic factor that affects 5-year OS. PGN significantly inhibited the cell proliferation activity of ESCC lines. This is the first study to show that high TLR6 expression status predicts a more favorable prognosis in locally advanced thoracic ESCC patients after curative esophagectomy. PGN released from “beneficial bacteria” seems to have potential to inhibit the cell proliferation activity of ESCC. MDPI 2023-05-04 /pmc/articles/PMC10217459/ /pubmed/37232814 http://dx.doi.org/10.3390/curroncol30050356 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sato, Yusuke
Wakita, Akiyuki
Maeda, Eri
Nagaki, Yushi
Sasamori, Ryohei
Kemuriyama, Kohei
Nozaki, Shu
Ito, Satoru
Terata, Kaori
Imai, Kazuhiro
Nanjo, Hiroshi
Nomura, Kyoko
Minamiya, Yoshihiro
High TLR6 Expression Status Predicts a More Favorable Prognosis after Esophagectomy for Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma
title High TLR6 Expression Status Predicts a More Favorable Prognosis after Esophagectomy for Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma
title_full High TLR6 Expression Status Predicts a More Favorable Prognosis after Esophagectomy for Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma
title_fullStr High TLR6 Expression Status Predicts a More Favorable Prognosis after Esophagectomy for Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma
title_full_unstemmed High TLR6 Expression Status Predicts a More Favorable Prognosis after Esophagectomy for Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma
title_short High TLR6 Expression Status Predicts a More Favorable Prognosis after Esophagectomy for Locally Advanced Thoracic Esophageal Squamous Cell Carcinoma
title_sort high tlr6 expression status predicts a more favorable prognosis after esophagectomy for locally advanced thoracic esophageal squamous cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217459/
https://www.ncbi.nlm.nih.gov/pubmed/37232814
http://dx.doi.org/10.3390/curroncol30050356
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