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Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model

Many probiotic bacteria have been proven to prevent allergic airway responses through immunomodulation. This study was conducted to evaluate the effects of heat-killed Bifidobacterium longum BBMN68 (BBMN68) in pasteurized yogurt on the alleviation of mugwort pollen (MP)-induced allergic inflammation...

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Autores principales: Niu, Xiaokang, Yin, Xindi, Wu, Xiuying, Zhang, Qi, Jiang, Yunyun, He, Jingjing, Zhao, Yuyang, Zhang, Chao, Ren, Yimei, Lai, Mengxuan, Sang, Yue, Wang, Ran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217734/
https://www.ncbi.nlm.nih.gov/pubmed/37238867
http://dx.doi.org/10.3390/foods12102049
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author Niu, Xiaokang
Yin, Xindi
Wu, Xiuying
Zhang, Qi
Jiang, Yunyun
He, Jingjing
Zhao, Yuyang
Zhang, Chao
Ren, Yimei
Lai, Mengxuan
Sang, Yue
Wang, Ran
author_facet Niu, Xiaokang
Yin, Xindi
Wu, Xiuying
Zhang, Qi
Jiang, Yunyun
He, Jingjing
Zhao, Yuyang
Zhang, Chao
Ren, Yimei
Lai, Mengxuan
Sang, Yue
Wang, Ran
author_sort Niu, Xiaokang
collection PubMed
description Many probiotic bacteria have been proven to prevent allergic airway responses through immunomodulation. This study was conducted to evaluate the effects of heat-killed Bifidobacterium longum BBMN68 (BBMN68) in pasteurized yogurt on the alleviation of mugwort pollen (MP)-induced allergic inflammation. BALB/c mice aged 5–6 weeks were randomly assigned and fed pasteurized yogurt containing heat-killed BBMN68 for 27 days, followed by allergic sensitization and challenge with MP extract. The allergic mice that received pasteurized yogurt containing heat-killed BBMN68 had improved immune status, including a lower serum IgE level, decreased serum interleukin (IL)-4, IL-5, and IL-13 concentrations, and alleviated airway inflammation manifested by increased macrophage and decreased eosinophil and neutrophil counts in BALF, as well as airway remodeling and suppressed peribronchial cellular infiltration. Moreover, oral administration of pasteurized yogurt containing heat-killed BBMN68 significantly modulated gut microbiota composition by influencing the proportion of beneficial genera associated with inflammation and immunity, such as Lactobacillus, Candidatus_Saccharimonas, Odoribacter, and Parabacteroides, which also negatively correlated with serum IgE and Th2 cytokine levels. These results demonstrated that pasteurized yogurt containing heat-killed BBMN68 had mitigative effects on allergic airway inflammation, likely through maintaining the systemic Th1/Th2 immune balance by altering the structure and function of the gut microbiota.
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spelling pubmed-102177342023-05-27 Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model Niu, Xiaokang Yin, Xindi Wu, Xiuying Zhang, Qi Jiang, Yunyun He, Jingjing Zhao, Yuyang Zhang, Chao Ren, Yimei Lai, Mengxuan Sang, Yue Wang, Ran Foods Article Many probiotic bacteria have been proven to prevent allergic airway responses through immunomodulation. This study was conducted to evaluate the effects of heat-killed Bifidobacterium longum BBMN68 (BBMN68) in pasteurized yogurt on the alleviation of mugwort pollen (MP)-induced allergic inflammation. BALB/c mice aged 5–6 weeks were randomly assigned and fed pasteurized yogurt containing heat-killed BBMN68 for 27 days, followed by allergic sensitization and challenge with MP extract. The allergic mice that received pasteurized yogurt containing heat-killed BBMN68 had improved immune status, including a lower serum IgE level, decreased serum interleukin (IL)-4, IL-5, and IL-13 concentrations, and alleviated airway inflammation manifested by increased macrophage and decreased eosinophil and neutrophil counts in BALF, as well as airway remodeling and suppressed peribronchial cellular infiltration. Moreover, oral administration of pasteurized yogurt containing heat-killed BBMN68 significantly modulated gut microbiota composition by influencing the proportion of beneficial genera associated with inflammation and immunity, such as Lactobacillus, Candidatus_Saccharimonas, Odoribacter, and Parabacteroides, which also negatively correlated with serum IgE and Th2 cytokine levels. These results demonstrated that pasteurized yogurt containing heat-killed BBMN68 had mitigative effects on allergic airway inflammation, likely through maintaining the systemic Th1/Th2 immune balance by altering the structure and function of the gut microbiota. MDPI 2023-05-19 /pmc/articles/PMC10217734/ /pubmed/37238867 http://dx.doi.org/10.3390/foods12102049 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Niu, Xiaokang
Yin, Xindi
Wu, Xiuying
Zhang, Qi
Jiang, Yunyun
He, Jingjing
Zhao, Yuyang
Zhang, Chao
Ren, Yimei
Lai, Mengxuan
Sang, Yue
Wang, Ran
Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model
title Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model
title_full Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model
title_fullStr Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model
title_full_unstemmed Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model
title_short Heat-Killed Bifidobacterium longum BBMN68 in Pasteurized Yogurt Alleviates Mugwort Pollen-Induced Allergic Airway Responses through Gut Microbiota Modulation in a Murine Model
title_sort heat-killed bifidobacterium longum bbmn68 in pasteurized yogurt alleviates mugwort pollen-induced allergic airway responses through gut microbiota modulation in a murine model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217734/
https://www.ncbi.nlm.nih.gov/pubmed/37238867
http://dx.doi.org/10.3390/foods12102049
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