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Generation and Characterization of a Transgenic Mouse That Specifically Expresses the Cre Recombinase in Spermatids
Spermiogenesis is the step during which post-meiotic cells, called spermatids, undergo numerous morphological changes and differentiate into spermatozoa. Thousands of genes have been described to be expressed at this stage and could contribute to spermatid differentiation. Genetically-engineered mou...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217838/ https://www.ncbi.nlm.nih.gov/pubmed/37239343 http://dx.doi.org/10.3390/genes14050983 |
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author | Gobé, Clara Ialy-Radio, Côme Pierre, Rémi Cocquet, Julie |
author_facet | Gobé, Clara Ialy-Radio, Côme Pierre, Rémi Cocquet, Julie |
author_sort | Gobé, Clara |
collection | PubMed |
description | Spermiogenesis is the step during which post-meiotic cells, called spermatids, undergo numerous morphological changes and differentiate into spermatozoa. Thousands of genes have been described to be expressed at this stage and could contribute to spermatid differentiation. Genetically-engineered mouse models using Cre/LoxP or CrispR/Cas9 are the favored approaches to characterize gene function and better understand the genetic basis of male infertility. In the present study, we produced a new spermatid-specific Cre transgenic mouse line, in which the improved iCre recombinase is expressed under the control of the acrosomal vesicle protein 1 gene promoter (Acrv1-iCre). We show that Cre protein expression is restricted to the testis and only detected in round spermatids of stage V to VIII seminiferous tubules. The Acrv1-iCre line can conditionally knockout a gene during spermiogenesis with a > 95% efficiency. Therefore, it could be useful to unravel the function of genes during the late stage of spermatogenesis, but it can also be used to produce an embryo with a paternally deleted allele without causing early spermatogenesis defects. |
format | Online Article Text |
id | pubmed-10217838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102178382023-05-27 Generation and Characterization of a Transgenic Mouse That Specifically Expresses the Cre Recombinase in Spermatids Gobé, Clara Ialy-Radio, Côme Pierre, Rémi Cocquet, Julie Genes (Basel) Article Spermiogenesis is the step during which post-meiotic cells, called spermatids, undergo numerous morphological changes and differentiate into spermatozoa. Thousands of genes have been described to be expressed at this stage and could contribute to spermatid differentiation. Genetically-engineered mouse models using Cre/LoxP or CrispR/Cas9 are the favored approaches to characterize gene function and better understand the genetic basis of male infertility. In the present study, we produced a new spermatid-specific Cre transgenic mouse line, in which the improved iCre recombinase is expressed under the control of the acrosomal vesicle protein 1 gene promoter (Acrv1-iCre). We show that Cre protein expression is restricted to the testis and only detected in round spermatids of stage V to VIII seminiferous tubules. The Acrv1-iCre line can conditionally knockout a gene during spermiogenesis with a > 95% efficiency. Therefore, it could be useful to unravel the function of genes during the late stage of spermatogenesis, but it can also be used to produce an embryo with a paternally deleted allele without causing early spermatogenesis defects. MDPI 2023-04-27 /pmc/articles/PMC10217838/ /pubmed/37239343 http://dx.doi.org/10.3390/genes14050983 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gobé, Clara Ialy-Radio, Côme Pierre, Rémi Cocquet, Julie Generation and Characterization of a Transgenic Mouse That Specifically Expresses the Cre Recombinase in Spermatids |
title | Generation and Characterization of a Transgenic Mouse That Specifically Expresses the Cre Recombinase in Spermatids |
title_full | Generation and Characterization of a Transgenic Mouse That Specifically Expresses the Cre Recombinase in Spermatids |
title_fullStr | Generation and Characterization of a Transgenic Mouse That Specifically Expresses the Cre Recombinase in Spermatids |
title_full_unstemmed | Generation and Characterization of a Transgenic Mouse That Specifically Expresses the Cre Recombinase in Spermatids |
title_short | Generation and Characterization of a Transgenic Mouse That Specifically Expresses the Cre Recombinase in Spermatids |
title_sort | generation and characterization of a transgenic mouse that specifically expresses the cre recombinase in spermatids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217838/ https://www.ncbi.nlm.nih.gov/pubmed/37239343 http://dx.doi.org/10.3390/genes14050983 |
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