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Antitumoral and Immunogenic Capacity of β-D-Glucose—Reduced Silver Nanoparticles in Breast Cancer

Immunogenic cell death (ICD) is a type of cell death capable of stimulating immunity against cancer through danger signals that lead to an adaptive immune response. Silver nanoparticles (AgNPs) have been shown to have a cytotoxic effect on cancer cells; however, their mechanism of action is not full...

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Autores principales: Félix-Piña, Pedro, Franco Molina, Moisés Armides, Zarate Triviño, Diana Ginette, García Coronado, Paola Leonor, Zapata Benavides, Pablo, Rodríguez Padilla, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217844/
https://www.ncbi.nlm.nih.gov/pubmed/37239831
http://dx.doi.org/10.3390/ijms24108485
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author Félix-Piña, Pedro
Franco Molina, Moisés Armides
Zarate Triviño, Diana Ginette
García Coronado, Paola Leonor
Zapata Benavides, Pablo
Rodríguez Padilla, Cristina
author_facet Félix-Piña, Pedro
Franco Molina, Moisés Armides
Zarate Triviño, Diana Ginette
García Coronado, Paola Leonor
Zapata Benavides, Pablo
Rodríguez Padilla, Cristina
author_sort Félix-Piña, Pedro
collection PubMed
description Immunogenic cell death (ICD) is a type of cell death capable of stimulating immunity against cancer through danger signals that lead to an adaptive immune response. Silver nanoparticles (AgNPs) have been shown to have a cytotoxic effect on cancer cells; however, their mechanism of action is not fully understood. The present study synthesized, characterized, and evaluated the cytotoxic effect of beta-D-glucose-reduced AgNPs (AgNPs-G) against breast cancer (BC) cells in vitro; and assess the immunogenicity of cell death in vitro and in vivo. The results showed that AgNPs-G induce cell death in a dose-dependent manner on BC cell lines. In addition, AgNPs show antiproliferative effects by interfering with the cell cycle. Regarding the detection of damage-associated molecular patterns (DAMPs), it was found that treatment with AgNPs-G induces calreticulin exposure and the release of HSP70, HSP90, HMGB1, and ATP. In vivo, prophylactic vaccination did not prevent tumor establishment; however, tumor weight was significantly lower in AgNPs-G vaccinated mice, while the survival rate increased. In conclusion, we have developed a new method for the synthesis of AgNPs-G, with in vitro antitumor cytotoxic activity on BC cells, accompanied by the release of DAMPs. In vivo, immunization with AgNPs-G failed to induce a complete immune response in mice. Consequently, additional studies are needed to elucidate the mechanism of cell death that leads to the design of strategies and combinations with clinical efficacy.
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spelling pubmed-102178442023-05-27 Antitumoral and Immunogenic Capacity of β-D-Glucose—Reduced Silver Nanoparticles in Breast Cancer Félix-Piña, Pedro Franco Molina, Moisés Armides Zarate Triviño, Diana Ginette García Coronado, Paola Leonor Zapata Benavides, Pablo Rodríguez Padilla, Cristina Int J Mol Sci Article Immunogenic cell death (ICD) is a type of cell death capable of stimulating immunity against cancer through danger signals that lead to an adaptive immune response. Silver nanoparticles (AgNPs) have been shown to have a cytotoxic effect on cancer cells; however, their mechanism of action is not fully understood. The present study synthesized, characterized, and evaluated the cytotoxic effect of beta-D-glucose-reduced AgNPs (AgNPs-G) against breast cancer (BC) cells in vitro; and assess the immunogenicity of cell death in vitro and in vivo. The results showed that AgNPs-G induce cell death in a dose-dependent manner on BC cell lines. In addition, AgNPs show antiproliferative effects by interfering with the cell cycle. Regarding the detection of damage-associated molecular patterns (DAMPs), it was found that treatment with AgNPs-G induces calreticulin exposure and the release of HSP70, HSP90, HMGB1, and ATP. In vivo, prophylactic vaccination did not prevent tumor establishment; however, tumor weight was significantly lower in AgNPs-G vaccinated mice, while the survival rate increased. In conclusion, we have developed a new method for the synthesis of AgNPs-G, with in vitro antitumor cytotoxic activity on BC cells, accompanied by the release of DAMPs. In vivo, immunization with AgNPs-G failed to induce a complete immune response in mice. Consequently, additional studies are needed to elucidate the mechanism of cell death that leads to the design of strategies and combinations with clinical efficacy. MDPI 2023-05-09 /pmc/articles/PMC10217844/ /pubmed/37239831 http://dx.doi.org/10.3390/ijms24108485 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Félix-Piña, Pedro
Franco Molina, Moisés Armides
Zarate Triviño, Diana Ginette
García Coronado, Paola Leonor
Zapata Benavides, Pablo
Rodríguez Padilla, Cristina
Antitumoral and Immunogenic Capacity of β-D-Glucose—Reduced Silver Nanoparticles in Breast Cancer
title Antitumoral and Immunogenic Capacity of β-D-Glucose—Reduced Silver Nanoparticles in Breast Cancer
title_full Antitumoral and Immunogenic Capacity of β-D-Glucose—Reduced Silver Nanoparticles in Breast Cancer
title_fullStr Antitumoral and Immunogenic Capacity of β-D-Glucose—Reduced Silver Nanoparticles in Breast Cancer
title_full_unstemmed Antitumoral and Immunogenic Capacity of β-D-Glucose—Reduced Silver Nanoparticles in Breast Cancer
title_short Antitumoral and Immunogenic Capacity of β-D-Glucose—Reduced Silver Nanoparticles in Breast Cancer
title_sort antitumoral and immunogenic capacity of β-d-glucose—reduced silver nanoparticles in breast cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217844/
https://www.ncbi.nlm.nih.gov/pubmed/37239831
http://dx.doi.org/10.3390/ijms24108485
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