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Decoding the Human Epidermal Complexity at Single-Cell Resolution
The epidermis is one of the largest tissues in the human body, serving as a protective barrier. The basal layer of the epidermis, which consists of epithelial stem cells and transient amplifying progenitors, represents its proliferative compartment. As keratinocytes migrate from the basal layer to t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217868/ https://www.ncbi.nlm.nih.gov/pubmed/37239891 http://dx.doi.org/10.3390/ijms24108544 |
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author | Polito, Maria Pia Marini, Grazia Palamenghi, Michele Enzo, Elena |
author_facet | Polito, Maria Pia Marini, Grazia Palamenghi, Michele Enzo, Elena |
author_sort | Polito, Maria Pia |
collection | PubMed |
description | The epidermis is one of the largest tissues in the human body, serving as a protective barrier. The basal layer of the epidermis, which consists of epithelial stem cells and transient amplifying progenitors, represents its proliferative compartment. As keratinocytes migrate from the basal layer to the skin surface, they exit the cell cycle and initiate terminal differentiation, ultimately generating the suprabasal epidermal layers. A deeper understanding of the molecular mechanisms and pathways driving keratinocytes’ organization and regeneration is essential for successful therapeutic approaches. Single-cell techniques are valuable tools for studying molecular heterogeneity. The high-resolution characterization obtained with these technologies has identified disease-specific drivers and new therapeutic targets, further promoting the advancement of personalized therapies. This review summarizes the latest findings on the transcriptomic and epigenetic profiling of human epidermal cells, analyzed from human biopsy or after in vitro cultivation, focusing on physiological, wound healing, and inflammatory skin conditions. |
format | Online Article Text |
id | pubmed-10217868 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102178682023-05-27 Decoding the Human Epidermal Complexity at Single-Cell Resolution Polito, Maria Pia Marini, Grazia Palamenghi, Michele Enzo, Elena Int J Mol Sci Review The epidermis is one of the largest tissues in the human body, serving as a protective barrier. The basal layer of the epidermis, which consists of epithelial stem cells and transient amplifying progenitors, represents its proliferative compartment. As keratinocytes migrate from the basal layer to the skin surface, they exit the cell cycle and initiate terminal differentiation, ultimately generating the suprabasal epidermal layers. A deeper understanding of the molecular mechanisms and pathways driving keratinocytes’ organization and regeneration is essential for successful therapeutic approaches. Single-cell techniques are valuable tools for studying molecular heterogeneity. The high-resolution characterization obtained with these technologies has identified disease-specific drivers and new therapeutic targets, further promoting the advancement of personalized therapies. This review summarizes the latest findings on the transcriptomic and epigenetic profiling of human epidermal cells, analyzed from human biopsy or after in vitro cultivation, focusing on physiological, wound healing, and inflammatory skin conditions. MDPI 2023-05-10 /pmc/articles/PMC10217868/ /pubmed/37239891 http://dx.doi.org/10.3390/ijms24108544 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Polito, Maria Pia Marini, Grazia Palamenghi, Michele Enzo, Elena Decoding the Human Epidermal Complexity at Single-Cell Resolution |
title | Decoding the Human Epidermal Complexity at Single-Cell Resolution |
title_full | Decoding the Human Epidermal Complexity at Single-Cell Resolution |
title_fullStr | Decoding the Human Epidermal Complexity at Single-Cell Resolution |
title_full_unstemmed | Decoding the Human Epidermal Complexity at Single-Cell Resolution |
title_short | Decoding the Human Epidermal Complexity at Single-Cell Resolution |
title_sort | decoding the human epidermal complexity at single-cell resolution |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217868/ https://www.ncbi.nlm.nih.gov/pubmed/37239891 http://dx.doi.org/10.3390/ijms24108544 |
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