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Decoding the Human Epidermal Complexity at Single-Cell Resolution

The epidermis is one of the largest tissues in the human body, serving as a protective barrier. The basal layer of the epidermis, which consists of epithelial stem cells and transient amplifying progenitors, represents its proliferative compartment. As keratinocytes migrate from the basal layer to t...

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Detalles Bibliográficos
Autores principales: Polito, Maria Pia, Marini, Grazia, Palamenghi, Michele, Enzo, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217868/
https://www.ncbi.nlm.nih.gov/pubmed/37239891
http://dx.doi.org/10.3390/ijms24108544
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author Polito, Maria Pia
Marini, Grazia
Palamenghi, Michele
Enzo, Elena
author_facet Polito, Maria Pia
Marini, Grazia
Palamenghi, Michele
Enzo, Elena
author_sort Polito, Maria Pia
collection PubMed
description The epidermis is one of the largest tissues in the human body, serving as a protective barrier. The basal layer of the epidermis, which consists of epithelial stem cells and transient amplifying progenitors, represents its proliferative compartment. As keratinocytes migrate from the basal layer to the skin surface, they exit the cell cycle and initiate terminal differentiation, ultimately generating the suprabasal epidermal layers. A deeper understanding of the molecular mechanisms and pathways driving keratinocytes’ organization and regeneration is essential for successful therapeutic approaches. Single-cell techniques are valuable tools for studying molecular heterogeneity. The high-resolution characterization obtained with these technologies has identified disease-specific drivers and new therapeutic targets, further promoting the advancement of personalized therapies. This review summarizes the latest findings on the transcriptomic and epigenetic profiling of human epidermal cells, analyzed from human biopsy or after in vitro cultivation, focusing on physiological, wound healing, and inflammatory skin conditions.
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spelling pubmed-102178682023-05-27 Decoding the Human Epidermal Complexity at Single-Cell Resolution Polito, Maria Pia Marini, Grazia Palamenghi, Michele Enzo, Elena Int J Mol Sci Review The epidermis is one of the largest tissues in the human body, serving as a protective barrier. The basal layer of the epidermis, which consists of epithelial stem cells and transient amplifying progenitors, represents its proliferative compartment. As keratinocytes migrate from the basal layer to the skin surface, they exit the cell cycle and initiate terminal differentiation, ultimately generating the suprabasal epidermal layers. A deeper understanding of the molecular mechanisms and pathways driving keratinocytes’ organization and regeneration is essential for successful therapeutic approaches. Single-cell techniques are valuable tools for studying molecular heterogeneity. The high-resolution characterization obtained with these technologies has identified disease-specific drivers and new therapeutic targets, further promoting the advancement of personalized therapies. This review summarizes the latest findings on the transcriptomic and epigenetic profiling of human epidermal cells, analyzed from human biopsy or after in vitro cultivation, focusing on physiological, wound healing, and inflammatory skin conditions. MDPI 2023-05-10 /pmc/articles/PMC10217868/ /pubmed/37239891 http://dx.doi.org/10.3390/ijms24108544 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Polito, Maria Pia
Marini, Grazia
Palamenghi, Michele
Enzo, Elena
Decoding the Human Epidermal Complexity at Single-Cell Resolution
title Decoding the Human Epidermal Complexity at Single-Cell Resolution
title_full Decoding the Human Epidermal Complexity at Single-Cell Resolution
title_fullStr Decoding the Human Epidermal Complexity at Single-Cell Resolution
title_full_unstemmed Decoding the Human Epidermal Complexity at Single-Cell Resolution
title_short Decoding the Human Epidermal Complexity at Single-Cell Resolution
title_sort decoding the human epidermal complexity at single-cell resolution
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10217868/
https://www.ncbi.nlm.nih.gov/pubmed/37239891
http://dx.doi.org/10.3390/ijms24108544
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