Cargando…
Genome-Wide Identification and Analysis of OsSPXs Revealed Its Genetic Influence on Cold Tolerance of Dongxiang Wild Rice (DXWR)
SPX-domain proteins (small proteins with only the SPX domain) have been proven to be involved in phosphate-related signal transduction and regulation pathways. Except for OsSPX1 research showing that it plays a role in the process of rice adaptation to cold stress, the potential functions of other S...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218074/ https://www.ncbi.nlm.nih.gov/pubmed/37240100 http://dx.doi.org/10.3390/ijms24108755 |
_version_ | 1785048687148269568 |
---|---|
author | Huang, Cheng Wang, Jilin Wang, Dianwen Chang, Jingjing Chen, Hongping Chen, Dazhou Deng, Wei Tian, Chunjie |
author_facet | Huang, Cheng Wang, Jilin Wang, Dianwen Chang, Jingjing Chen, Hongping Chen, Dazhou Deng, Wei Tian, Chunjie |
author_sort | Huang, Cheng |
collection | PubMed |
description | SPX-domain proteins (small proteins with only the SPX domain) have been proven to be involved in phosphate-related signal transduction and regulation pathways. Except for OsSPX1 research showing that it plays a role in the process of rice adaptation to cold stress, the potential functions of other SPX genes in cold stress are unknown. Therefore, in this study, we identified six OsSPXs from the whole genome of DXWR. The phylogeny of OsSPXs has a strong correlation with its motif. Transcriptome data analysis showed that OsSPXs were highly sensitive to cold stress, and real-time PCR verified that the levels of OsSPX1, OsSPX2, OsSPX4, and OsSPX6 in cold-tolerant materials (DXWR) during cold treatment were higher than that of cold-sensitive rice (GZX49). The promoter region of DXWR OsSPXs contains a large number of cis-acting elements related to abiotic stress tolerance and plant hormone response. At the same time, these genes have expression patterns that are highly similar to cold-tolerance genes. This study provides useful information about OsSPXs, which is helpful for the gene-function research of DXWR and genetic improvements during breeding. |
format | Online Article Text |
id | pubmed-10218074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102180742023-05-27 Genome-Wide Identification and Analysis of OsSPXs Revealed Its Genetic Influence on Cold Tolerance of Dongxiang Wild Rice (DXWR) Huang, Cheng Wang, Jilin Wang, Dianwen Chang, Jingjing Chen, Hongping Chen, Dazhou Deng, Wei Tian, Chunjie Int J Mol Sci Article SPX-domain proteins (small proteins with only the SPX domain) have been proven to be involved in phosphate-related signal transduction and regulation pathways. Except for OsSPX1 research showing that it plays a role in the process of rice adaptation to cold stress, the potential functions of other SPX genes in cold stress are unknown. Therefore, in this study, we identified six OsSPXs from the whole genome of DXWR. The phylogeny of OsSPXs has a strong correlation with its motif. Transcriptome data analysis showed that OsSPXs were highly sensitive to cold stress, and real-time PCR verified that the levels of OsSPX1, OsSPX2, OsSPX4, and OsSPX6 in cold-tolerant materials (DXWR) during cold treatment were higher than that of cold-sensitive rice (GZX49). The promoter region of DXWR OsSPXs contains a large number of cis-acting elements related to abiotic stress tolerance and plant hormone response. At the same time, these genes have expression patterns that are highly similar to cold-tolerance genes. This study provides useful information about OsSPXs, which is helpful for the gene-function research of DXWR and genetic improvements during breeding. MDPI 2023-05-15 /pmc/articles/PMC10218074/ /pubmed/37240100 http://dx.doi.org/10.3390/ijms24108755 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Huang, Cheng Wang, Jilin Wang, Dianwen Chang, Jingjing Chen, Hongping Chen, Dazhou Deng, Wei Tian, Chunjie Genome-Wide Identification and Analysis of OsSPXs Revealed Its Genetic Influence on Cold Tolerance of Dongxiang Wild Rice (DXWR) |
title | Genome-Wide Identification and Analysis of OsSPXs Revealed Its Genetic Influence on Cold Tolerance of Dongxiang Wild Rice (DXWR) |
title_full | Genome-Wide Identification and Analysis of OsSPXs Revealed Its Genetic Influence on Cold Tolerance of Dongxiang Wild Rice (DXWR) |
title_fullStr | Genome-Wide Identification and Analysis of OsSPXs Revealed Its Genetic Influence on Cold Tolerance of Dongxiang Wild Rice (DXWR) |
title_full_unstemmed | Genome-Wide Identification and Analysis of OsSPXs Revealed Its Genetic Influence on Cold Tolerance of Dongxiang Wild Rice (DXWR) |
title_short | Genome-Wide Identification and Analysis of OsSPXs Revealed Its Genetic Influence on Cold Tolerance of Dongxiang Wild Rice (DXWR) |
title_sort | genome-wide identification and analysis of osspxs revealed its genetic influence on cold tolerance of dongxiang wild rice (dxwr) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218074/ https://www.ncbi.nlm.nih.gov/pubmed/37240100 http://dx.doi.org/10.3390/ijms24108755 |
work_keys_str_mv | AT huangcheng genomewideidentificationandanalysisofosspxsrevealeditsgeneticinfluenceoncoldtoleranceofdongxiangwildricedxwr AT wangjilin genomewideidentificationandanalysisofosspxsrevealeditsgeneticinfluenceoncoldtoleranceofdongxiangwildricedxwr AT wangdianwen genomewideidentificationandanalysisofosspxsrevealeditsgeneticinfluenceoncoldtoleranceofdongxiangwildricedxwr AT changjingjing genomewideidentificationandanalysisofosspxsrevealeditsgeneticinfluenceoncoldtoleranceofdongxiangwildricedxwr AT chenhongping genomewideidentificationandanalysisofosspxsrevealeditsgeneticinfluenceoncoldtoleranceofdongxiangwildricedxwr AT chendazhou genomewideidentificationandanalysisofosspxsrevealeditsgeneticinfluenceoncoldtoleranceofdongxiangwildricedxwr AT dengwei genomewideidentificationandanalysisofosspxsrevealeditsgeneticinfluenceoncoldtoleranceofdongxiangwildricedxwr AT tianchunjie genomewideidentificationandanalysisofosspxsrevealeditsgeneticinfluenceoncoldtoleranceofdongxiangwildricedxwr |