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Multiplexed Gene Engineering Based on dCas9 and gRNA-tRNA Array Encoded on Single Transcript
Simultaneously, multiplexed genome engineering and targeting multiple genomic loci are valuable to elucidating gene interactions and characterizing genetic networks that affect phenotypes. Here, we developed a general CRISPR-based platform to perform four functions and target multiple genome loci en...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218229/ https://www.ncbi.nlm.nih.gov/pubmed/37239880 http://dx.doi.org/10.3390/ijms24108535 |