Multiplexed Gene Engineering Based on dCas9 and gRNA-tRNA Array Encoded on Single Transcript

Simultaneously, multiplexed genome engineering and targeting multiple genomic loci are valuable to elucidating gene interactions and characterizing genetic networks that affect phenotypes. Here, we developed a general CRISPR-based platform to perform four functions and target multiple genome loci en...

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Detalles Bibliográficos
Autores principales: Jiang, Chaoqian, Geng, Lishuang, Wang, Jinpeng, Liang, Yingjuan, Guo, Xiaochen, Liu, Chang, Zhao, Yunjing, Jin, Junxue, Liu, Zhonghua, Mu, Yanshuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218229/
https://www.ncbi.nlm.nih.gov/pubmed/37239880
http://dx.doi.org/10.3390/ijms24108535

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