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Temperature-Dependent Effects of Eicosapentaenoic Acid (EPA) on Browning of Subcutaneous Adipose Tissue in UCP1 Knockout Male Mice

Uncoupling protein 1 (UCP1) plays a central role in thermogenic tissues by uncoupling cellular respiration to dissipate energy. Beige adipocytes, an inducible form of thermogenic cells in subcutaneous adipose tissue (SAT), have become a major focus in obesity research. We have previously shown that...

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Autores principales: Zu, Yujiao, Pahlavani, Mandana, Ramalingam, Latha, Jayarathne, Shasika, Andrade, Jose, Scoggin, Shane, Festuccia, William T., Kalupahana, Nishan S., Moustaid-Moussa, Naima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218247/
https://www.ncbi.nlm.nih.gov/pubmed/37240054
http://dx.doi.org/10.3390/ijms24108708
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author Zu, Yujiao
Pahlavani, Mandana
Ramalingam, Latha
Jayarathne, Shasika
Andrade, Jose
Scoggin, Shane
Festuccia, William T.
Kalupahana, Nishan S.
Moustaid-Moussa, Naima
author_facet Zu, Yujiao
Pahlavani, Mandana
Ramalingam, Latha
Jayarathne, Shasika
Andrade, Jose
Scoggin, Shane
Festuccia, William T.
Kalupahana, Nishan S.
Moustaid-Moussa, Naima
author_sort Zu, Yujiao
collection PubMed
description Uncoupling protein 1 (UCP1) plays a central role in thermogenic tissues by uncoupling cellular respiration to dissipate energy. Beige adipocytes, an inducible form of thermogenic cells in subcutaneous adipose tissue (SAT), have become a major focus in obesity research. We have previously shown that eicosapentaenoic acid (EPA) ameliorated high-fat diet (HFD)-induced obesity by activating brown fat in C57BL/6J (B6) mice at thermoneutrality (30 °C), independently of UCP1. Here, we investigated whether ambient temperature (22 °C) impacts EPA effects on SAT browning in wild-type (WT) and UCP1 knockout (KO) male mice and dissected underlying mechanisms using a cell model. We observed resistance to diet-induced obesity in UCP1 KO mice fed HFD at ambient temperature, with significantly higher expression of UCP1-independent thermogenic markers, compared to WT mice. These markers included the fibroblast growth factor 21 (FGF21) and sarco/endoplasmic reticulum Ca(2+)-ATPase 2b (SERCA2b), suggesting the indispensable role of temperature in beige fat reprogramming. Surprisingly, although EPA induced thermogenic effects in SAT-derived adipocytes harvested from both KO and WT mice, EPA only increased thermogenic gene and protein expression in the SAT of UCP1 KO mice housed at ambient temperature. Collectively, our findings indicate that the thermogenic effects of EPA, which are independent of UCP1, occur in a temperature-dependent manner.
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spelling pubmed-102182472023-05-27 Temperature-Dependent Effects of Eicosapentaenoic Acid (EPA) on Browning of Subcutaneous Adipose Tissue in UCP1 Knockout Male Mice Zu, Yujiao Pahlavani, Mandana Ramalingam, Latha Jayarathne, Shasika Andrade, Jose Scoggin, Shane Festuccia, William T. Kalupahana, Nishan S. Moustaid-Moussa, Naima Int J Mol Sci Article Uncoupling protein 1 (UCP1) plays a central role in thermogenic tissues by uncoupling cellular respiration to dissipate energy. Beige adipocytes, an inducible form of thermogenic cells in subcutaneous adipose tissue (SAT), have become a major focus in obesity research. We have previously shown that eicosapentaenoic acid (EPA) ameliorated high-fat diet (HFD)-induced obesity by activating brown fat in C57BL/6J (B6) mice at thermoneutrality (30 °C), independently of UCP1. Here, we investigated whether ambient temperature (22 °C) impacts EPA effects on SAT browning in wild-type (WT) and UCP1 knockout (KO) male mice and dissected underlying mechanisms using a cell model. We observed resistance to diet-induced obesity in UCP1 KO mice fed HFD at ambient temperature, with significantly higher expression of UCP1-independent thermogenic markers, compared to WT mice. These markers included the fibroblast growth factor 21 (FGF21) and sarco/endoplasmic reticulum Ca(2+)-ATPase 2b (SERCA2b), suggesting the indispensable role of temperature in beige fat reprogramming. Surprisingly, although EPA induced thermogenic effects in SAT-derived adipocytes harvested from both KO and WT mice, EPA only increased thermogenic gene and protein expression in the SAT of UCP1 KO mice housed at ambient temperature. Collectively, our findings indicate that the thermogenic effects of EPA, which are independent of UCP1, occur in a temperature-dependent manner. MDPI 2023-05-13 /pmc/articles/PMC10218247/ /pubmed/37240054 http://dx.doi.org/10.3390/ijms24108708 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zu, Yujiao
Pahlavani, Mandana
Ramalingam, Latha
Jayarathne, Shasika
Andrade, Jose
Scoggin, Shane
Festuccia, William T.
Kalupahana, Nishan S.
Moustaid-Moussa, Naima
Temperature-Dependent Effects of Eicosapentaenoic Acid (EPA) on Browning of Subcutaneous Adipose Tissue in UCP1 Knockout Male Mice
title Temperature-Dependent Effects of Eicosapentaenoic Acid (EPA) on Browning of Subcutaneous Adipose Tissue in UCP1 Knockout Male Mice
title_full Temperature-Dependent Effects of Eicosapentaenoic Acid (EPA) on Browning of Subcutaneous Adipose Tissue in UCP1 Knockout Male Mice
title_fullStr Temperature-Dependent Effects of Eicosapentaenoic Acid (EPA) on Browning of Subcutaneous Adipose Tissue in UCP1 Knockout Male Mice
title_full_unstemmed Temperature-Dependent Effects of Eicosapentaenoic Acid (EPA) on Browning of Subcutaneous Adipose Tissue in UCP1 Knockout Male Mice
title_short Temperature-Dependent Effects of Eicosapentaenoic Acid (EPA) on Browning of Subcutaneous Adipose Tissue in UCP1 Knockout Male Mice
title_sort temperature-dependent effects of eicosapentaenoic acid (epa) on browning of subcutaneous adipose tissue in ucp1 knockout male mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218247/
https://www.ncbi.nlm.nih.gov/pubmed/37240054
http://dx.doi.org/10.3390/ijms24108708
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