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Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation

Klebsiella pneumoniae is an opportunistic pathogen that can produce moderate and severe infections in immunosuppressed hosts. In recent years, an increase in the isolation of hypermucoviscous carbapenem-resistant K. pneumoniae with sequence type 25 (ST25) in hospitals in Norwest Argentina was observ...

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Autores principales: Dentice Maidana, Stefania, Elean, Mariano, Fukuyama, Kohtaro, Imamura, Yoshiya, Albarracín, Leonardo, Saha, Sudeb, Suda, Yoshihito, Kurata, Shoichiro, Jure, María Ángela, Kitazawa, Haruki, Villena, Julio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218288/
https://www.ncbi.nlm.nih.gov/pubmed/37240146
http://dx.doi.org/10.3390/ijms24108804
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author Dentice Maidana, Stefania
Elean, Mariano
Fukuyama, Kohtaro
Imamura, Yoshiya
Albarracín, Leonardo
Saha, Sudeb
Suda, Yoshihito
Kurata, Shoichiro
Jure, María Ángela
Kitazawa, Haruki
Villena, Julio
author_facet Dentice Maidana, Stefania
Elean, Mariano
Fukuyama, Kohtaro
Imamura, Yoshiya
Albarracín, Leonardo
Saha, Sudeb
Suda, Yoshihito
Kurata, Shoichiro
Jure, María Ángela
Kitazawa, Haruki
Villena, Julio
author_sort Dentice Maidana, Stefania
collection PubMed
description Klebsiella pneumoniae is an opportunistic pathogen that can produce moderate and severe infections in immunosuppressed hosts. In recent years, an increase in the isolation of hypermucoviscous carbapenem-resistant K. pneumoniae with sequence type 25 (ST25) in hospitals in Norwest Argentina was observed. This work aimed to study the virulence and inflammatory potential of two K. pneumoniae ST25 strains (LABACER01 and LABACER27) in the intestinal mucosa. The human intestinal Caco-2 cells were infected with the K. pneumoniae ST25 strains, and their adhesion and invasion rates and changes in the expression of tight junction and inflammatory factors genes were evaluated. ST25 strains were able to adhere and invade Caco-2 cells, reducing their viability. Furthermore, both strains reduced the expression of tight junction proteins (occludin, ZO-1, and claudin-5), altered permeability, and increased the expression of TGF-β and TLL1 and the inflammatory factors (COX-2, iNOS, MCP-1, IL-6, IL-8, and TNF-α) in Caco-2 cells. The inflammatory response induced by LABACER01 and LABACER27 was significantly lower than the one produced by LPS or other intestinal pathogens, including K. pneumoniae NTUH-K2044. No differences in virulence and inflammatory potential were found between LABACER01 and LABACER27. In line with these findings, no major differences between the strains were found when the comparative genomic analysis of virulence factors associated with intestinal infection/colonization was performed. This work is the first to demonstrate that hypermucoviscous carbapenem-resistant K. pneumoniae ST25 infects human intestinal epithelial cells and induces moderate inflammation.
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spelling pubmed-102182882023-05-27 Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation Dentice Maidana, Stefania Elean, Mariano Fukuyama, Kohtaro Imamura, Yoshiya Albarracín, Leonardo Saha, Sudeb Suda, Yoshihito Kurata, Shoichiro Jure, María Ángela Kitazawa, Haruki Villena, Julio Int J Mol Sci Article Klebsiella pneumoniae is an opportunistic pathogen that can produce moderate and severe infections in immunosuppressed hosts. In recent years, an increase in the isolation of hypermucoviscous carbapenem-resistant K. pneumoniae with sequence type 25 (ST25) in hospitals in Norwest Argentina was observed. This work aimed to study the virulence and inflammatory potential of two K. pneumoniae ST25 strains (LABACER01 and LABACER27) in the intestinal mucosa. The human intestinal Caco-2 cells were infected with the K. pneumoniae ST25 strains, and their adhesion and invasion rates and changes in the expression of tight junction and inflammatory factors genes were evaluated. ST25 strains were able to adhere and invade Caco-2 cells, reducing their viability. Furthermore, both strains reduced the expression of tight junction proteins (occludin, ZO-1, and claudin-5), altered permeability, and increased the expression of TGF-β and TLL1 and the inflammatory factors (COX-2, iNOS, MCP-1, IL-6, IL-8, and TNF-α) in Caco-2 cells. The inflammatory response induced by LABACER01 and LABACER27 was significantly lower than the one produced by LPS or other intestinal pathogens, including K. pneumoniae NTUH-K2044. No differences in virulence and inflammatory potential were found between LABACER01 and LABACER27. In line with these findings, no major differences between the strains were found when the comparative genomic analysis of virulence factors associated with intestinal infection/colonization was performed. This work is the first to demonstrate that hypermucoviscous carbapenem-resistant K. pneumoniae ST25 infects human intestinal epithelial cells and induces moderate inflammation. MDPI 2023-05-15 /pmc/articles/PMC10218288/ /pubmed/37240146 http://dx.doi.org/10.3390/ijms24108804 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dentice Maidana, Stefania
Elean, Mariano
Fukuyama, Kohtaro
Imamura, Yoshiya
Albarracín, Leonardo
Saha, Sudeb
Suda, Yoshihito
Kurata, Shoichiro
Jure, María Ángela
Kitazawa, Haruki
Villena, Julio
Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation
title Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation
title_full Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation
title_fullStr Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation
title_full_unstemmed Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation
title_short Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation
title_sort hypermucoviscous carbapenem-resistant klebsiella pneumoniae st25 infect human intestinal epithelial cells and induce moderate inflammation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218288/
https://www.ncbi.nlm.nih.gov/pubmed/37240146
http://dx.doi.org/10.3390/ijms24108804
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