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TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms

Alternative splicing is a part of mRNA processing that expands the diversity of proteins encoded by a single gene. Studying the full range of proteins–products of translation of alternatively spliced mRNA is extremely important for understanding the interactions between receptor proteins and ligands...

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Autores principales: Perik-Zavodskaia, Olga, Zhukova, Julia, Perik-Zavodskii, Roman, Alshevskaya, Alina, Lopatnikova, Julia, Sennikov, Sergey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218394/
https://www.ncbi.nlm.nih.gov/pubmed/37239433
http://dx.doi.org/10.3390/genes14051074
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author Perik-Zavodskaia, Olga
Zhukova, Julia
Perik-Zavodskii, Roman
Alshevskaya, Alina
Lopatnikova, Julia
Sennikov, Sergey
author_facet Perik-Zavodskaia, Olga
Zhukova, Julia
Perik-Zavodskii, Roman
Alshevskaya, Alina
Lopatnikova, Julia
Sennikov, Sergey
author_sort Perik-Zavodskaia, Olga
collection PubMed
description Alternative splicing is a part of mRNA processing that expands the diversity of proteins encoded by a single gene. Studying the full range of proteins–products of translation of alternatively spliced mRNA is extremely important for understanding the interactions between receptor proteins and ligands since different receptor protein isoforms can provide variation in the activation of signaling pathways. In this study, we investigated the expression of isoforms of TNFR1 and TNFR2 receptors before and after exposure to TNFα in two cell lines that had previously demonstrated diverse effects on cell proliferation under TNFα incubation using RT-qPCR. We found that after incubation with TNFα: (1) expression of isoform 3 of the TNFRSF1A gene was increased in both cell lines; (2) the cell line with increased proliferation, K562, had decreased expression of isoforms 1 and 4 of the TNFRSF1A gene and expression of isoform 2 of TNFRSF1B gene was absent at all; (3) the cell line with decreased proliferation—MCF-7 had significantly increased expression of isoform 2 of TNFRSF1B gene. Thus, we can conclude that TNFα exposure to the K562 and MCF-7 cell lines leads to changes in the expression of TNFα receptor isoforms, which, in turn, can appear via diverse proliferative effects.
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spelling pubmed-102183942023-05-27 TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms Perik-Zavodskaia, Olga Zhukova, Julia Perik-Zavodskii, Roman Alshevskaya, Alina Lopatnikova, Julia Sennikov, Sergey Genes (Basel) Communication Alternative splicing is a part of mRNA processing that expands the diversity of proteins encoded by a single gene. Studying the full range of proteins–products of translation of alternatively spliced mRNA is extremely important for understanding the interactions between receptor proteins and ligands since different receptor protein isoforms can provide variation in the activation of signaling pathways. In this study, we investigated the expression of isoforms of TNFR1 and TNFR2 receptors before and after exposure to TNFα in two cell lines that had previously demonstrated diverse effects on cell proliferation under TNFα incubation using RT-qPCR. We found that after incubation with TNFα: (1) expression of isoform 3 of the TNFRSF1A gene was increased in both cell lines; (2) the cell line with increased proliferation, K562, had decreased expression of isoforms 1 and 4 of the TNFRSF1A gene and expression of isoform 2 of TNFRSF1B gene was absent at all; (3) the cell line with decreased proliferation—MCF-7 had significantly increased expression of isoform 2 of TNFRSF1B gene. Thus, we can conclude that TNFα exposure to the K562 and MCF-7 cell lines leads to changes in the expression of TNFα receptor isoforms, which, in turn, can appear via diverse proliferative effects. MDPI 2023-05-12 /pmc/articles/PMC10218394/ /pubmed/37239433 http://dx.doi.org/10.3390/genes14051074 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Perik-Zavodskaia, Olga
Zhukova, Julia
Perik-Zavodskii, Roman
Alshevskaya, Alina
Lopatnikova, Julia
Sennikov, Sergey
TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms
title TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms
title_full TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms
title_fullStr TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms
title_full_unstemmed TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms
title_short TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms
title_sort tnfα causes a shift in gene expression of tnfrsf1a and tnfrsf1b isoforms
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218394/
https://www.ncbi.nlm.nih.gov/pubmed/37239433
http://dx.doi.org/10.3390/genes14051074
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