Cargando…
TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms
Alternative splicing is a part of mRNA processing that expands the diversity of proteins encoded by a single gene. Studying the full range of proteins–products of translation of alternatively spliced mRNA is extremely important for understanding the interactions between receptor proteins and ligands...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218394/ https://www.ncbi.nlm.nih.gov/pubmed/37239433 http://dx.doi.org/10.3390/genes14051074 |
_version_ | 1785048762978140160 |
---|---|
author | Perik-Zavodskaia, Olga Zhukova, Julia Perik-Zavodskii, Roman Alshevskaya, Alina Lopatnikova, Julia Sennikov, Sergey |
author_facet | Perik-Zavodskaia, Olga Zhukova, Julia Perik-Zavodskii, Roman Alshevskaya, Alina Lopatnikova, Julia Sennikov, Sergey |
author_sort | Perik-Zavodskaia, Olga |
collection | PubMed |
description | Alternative splicing is a part of mRNA processing that expands the diversity of proteins encoded by a single gene. Studying the full range of proteins–products of translation of alternatively spliced mRNA is extremely important for understanding the interactions between receptor proteins and ligands since different receptor protein isoforms can provide variation in the activation of signaling pathways. In this study, we investigated the expression of isoforms of TNFR1 and TNFR2 receptors before and after exposure to TNFα in two cell lines that had previously demonstrated diverse effects on cell proliferation under TNFα incubation using RT-qPCR. We found that after incubation with TNFα: (1) expression of isoform 3 of the TNFRSF1A gene was increased in both cell lines; (2) the cell line with increased proliferation, K562, had decreased expression of isoforms 1 and 4 of the TNFRSF1A gene and expression of isoform 2 of TNFRSF1B gene was absent at all; (3) the cell line with decreased proliferation—MCF-7 had significantly increased expression of isoform 2 of TNFRSF1B gene. Thus, we can conclude that TNFα exposure to the K562 and MCF-7 cell lines leads to changes in the expression of TNFα receptor isoforms, which, in turn, can appear via diverse proliferative effects. |
format | Online Article Text |
id | pubmed-10218394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102183942023-05-27 TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms Perik-Zavodskaia, Olga Zhukova, Julia Perik-Zavodskii, Roman Alshevskaya, Alina Lopatnikova, Julia Sennikov, Sergey Genes (Basel) Communication Alternative splicing is a part of mRNA processing that expands the diversity of proteins encoded by a single gene. Studying the full range of proteins–products of translation of alternatively spliced mRNA is extremely important for understanding the interactions between receptor proteins and ligands since different receptor protein isoforms can provide variation in the activation of signaling pathways. In this study, we investigated the expression of isoforms of TNFR1 and TNFR2 receptors before and after exposure to TNFα in two cell lines that had previously demonstrated diverse effects on cell proliferation under TNFα incubation using RT-qPCR. We found that after incubation with TNFα: (1) expression of isoform 3 of the TNFRSF1A gene was increased in both cell lines; (2) the cell line with increased proliferation, K562, had decreased expression of isoforms 1 and 4 of the TNFRSF1A gene and expression of isoform 2 of TNFRSF1B gene was absent at all; (3) the cell line with decreased proliferation—MCF-7 had significantly increased expression of isoform 2 of TNFRSF1B gene. Thus, we can conclude that TNFα exposure to the K562 and MCF-7 cell lines leads to changes in the expression of TNFα receptor isoforms, which, in turn, can appear via diverse proliferative effects. MDPI 2023-05-12 /pmc/articles/PMC10218394/ /pubmed/37239433 http://dx.doi.org/10.3390/genes14051074 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Perik-Zavodskaia, Olga Zhukova, Julia Perik-Zavodskii, Roman Alshevskaya, Alina Lopatnikova, Julia Sennikov, Sergey TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms |
title | TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms |
title_full | TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms |
title_fullStr | TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms |
title_full_unstemmed | TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms |
title_short | TNFα Causes a Shift in Gene Expression of TNFRSF1A and TNFRSF1B Isoforms |
title_sort | tnfα causes a shift in gene expression of tnfrsf1a and tnfrsf1b isoforms |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218394/ https://www.ncbi.nlm.nih.gov/pubmed/37239433 http://dx.doi.org/10.3390/genes14051074 |
work_keys_str_mv | AT perikzavodskaiaolga tnfacausesashiftingeneexpressionoftnfrsf1aandtnfrsf1bisoforms AT zhukovajulia tnfacausesashiftingeneexpressionoftnfrsf1aandtnfrsf1bisoforms AT perikzavodskiiroman tnfacausesashiftingeneexpressionoftnfrsf1aandtnfrsf1bisoforms AT alshevskayaalina tnfacausesashiftingeneexpressionoftnfrsf1aandtnfrsf1bisoforms AT lopatnikovajulia tnfacausesashiftingeneexpressionoftnfrsf1aandtnfrsf1bisoforms AT sennikovsergey tnfacausesashiftingeneexpressionoftnfrsf1aandtnfrsf1bisoforms |