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Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis
Male infertility is a global issue that seriously affects reproductive health. This study aimed to understand the underlying causes of idiopathic non-obstructive azoospermia (iNOA), which is a type of male infertility with unknown origins that accounts for 10–15% of cases. By using single-cell analy...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218437/ https://www.ncbi.nlm.nih.gov/pubmed/37240164 http://dx.doi.org/10.3390/ijms24108819 |
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author | Xia, Peng Ouyang, Siwei Shen, Rong Guo, Zhao Zhang, Guokun Liu, Xiangwen Yang, Xuguang Xie, Kun Wang, Degui |
author_facet | Xia, Peng Ouyang, Siwei Shen, Rong Guo, Zhao Zhang, Guokun Liu, Xiangwen Yang, Xuguang Xie, Kun Wang, Degui |
author_sort | Xia, Peng |
collection | PubMed |
description | Male infertility is a global issue that seriously affects reproductive health. This study aimed to understand the underlying causes of idiopathic non-obstructive azoospermia (iNOA), which is a type of male infertility with unknown origins that accounts for 10–15% of cases. By using single-cell analysis techniques, we aimed to uncover the mechanisms of iNOA and gain insight into the cellular and molecular changes in the testicular environment. In this study, we performed bioinformatics analysis using scRNA-seq and microarray data obtained from the GEO database. The analysis included techniques such as pseudotime analysis, cell–cell communication, and hdWGCNA. Our study showed a significant difference between the iNOA and the normal groups, indicating a disorder in the spermatogenic microenvironment in iNOA. We observed a reduction in the proportion of Sertoli cells and blocked germ cell differentiation. Additionally, we found evidence of testicular inflammation related to macrophages and identified ODF2 and CABYR as potential biomarkers for iNOA. |
format | Online Article Text |
id | pubmed-10218437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102184372023-05-27 Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis Xia, Peng Ouyang, Siwei Shen, Rong Guo, Zhao Zhang, Guokun Liu, Xiangwen Yang, Xuguang Xie, Kun Wang, Degui Int J Mol Sci Article Male infertility is a global issue that seriously affects reproductive health. This study aimed to understand the underlying causes of idiopathic non-obstructive azoospermia (iNOA), which is a type of male infertility with unknown origins that accounts for 10–15% of cases. By using single-cell analysis techniques, we aimed to uncover the mechanisms of iNOA and gain insight into the cellular and molecular changes in the testicular environment. In this study, we performed bioinformatics analysis using scRNA-seq and microarray data obtained from the GEO database. The analysis included techniques such as pseudotime analysis, cell–cell communication, and hdWGCNA. Our study showed a significant difference between the iNOA and the normal groups, indicating a disorder in the spermatogenic microenvironment in iNOA. We observed a reduction in the proportion of Sertoli cells and blocked germ cell differentiation. Additionally, we found evidence of testicular inflammation related to macrophages and identified ODF2 and CABYR as potential biomarkers for iNOA. MDPI 2023-05-16 /pmc/articles/PMC10218437/ /pubmed/37240164 http://dx.doi.org/10.3390/ijms24108819 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xia, Peng Ouyang, Siwei Shen, Rong Guo, Zhao Zhang, Guokun Liu, Xiangwen Yang, Xuguang Xie, Kun Wang, Degui Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis |
title | Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis |
title_full | Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis |
title_fullStr | Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis |
title_full_unstemmed | Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis |
title_short | Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis |
title_sort | macrophage-related testicular inflammation in individuals with idiopathic non-obstructive azoospermia: a single-cell analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218437/ https://www.ncbi.nlm.nih.gov/pubmed/37240164 http://dx.doi.org/10.3390/ijms24108819 |
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