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Small RNA Profiling of Aster Yellows Phytoplasma-Infected Catharanthus roseus Plants Showing Different Symptoms

Micropropagated Catharantus roseus plants infected with ‘Candidatus Phytoplasma asteris’ showed virescence symptoms, witches’ broom symptoms, or became asymptomatic after their planting in pots. Nine plants were grouped into three categories according to these symptoms, which were then employed for...

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Autores principales: Contaldo, Nicoletta, Zambon, Yuri, Galbacs, Zsuszanna Nagyne, Miloro, Fabio, Havelda, Zoltan, Bertaccini, Assunta, Varallyay, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218541/
https://www.ncbi.nlm.nih.gov/pubmed/37239473
http://dx.doi.org/10.3390/genes14051114
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author Contaldo, Nicoletta
Zambon, Yuri
Galbacs, Zsuszanna Nagyne
Miloro, Fabio
Havelda, Zoltan
Bertaccini, Assunta
Varallyay, Eva
author_facet Contaldo, Nicoletta
Zambon, Yuri
Galbacs, Zsuszanna Nagyne
Miloro, Fabio
Havelda, Zoltan
Bertaccini, Assunta
Varallyay, Eva
author_sort Contaldo, Nicoletta
collection PubMed
description Micropropagated Catharantus roseus plants infected with ‘Candidatus Phytoplasma asteris’ showed virescence symptoms, witches’ broom symptoms, or became asymptomatic after their planting in pots. Nine plants were grouped into three categories according to these symptoms, which were then employed for investigation. The phytoplasma concentration, as determined by qPCR, correlated well with the severity of symptoms. To reveal the changes in the small RNA profiles in these plants, small RNA high-throughput sequencing (HTS) was carried out. The bioinformatics comparison of the micro (mi) RNA and small interfering (si) RNA profiles of the symptomatic and asymptomatic plants showed changes, which could be correlated to some of the observed symptoms. These results complement previous studies on phytoplasmas and serve as a starting point for small RNA-omic studies in phytoplasma research.
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spelling pubmed-102185412023-05-27 Small RNA Profiling of Aster Yellows Phytoplasma-Infected Catharanthus roseus Plants Showing Different Symptoms Contaldo, Nicoletta Zambon, Yuri Galbacs, Zsuszanna Nagyne Miloro, Fabio Havelda, Zoltan Bertaccini, Assunta Varallyay, Eva Genes (Basel) Article Micropropagated Catharantus roseus plants infected with ‘Candidatus Phytoplasma asteris’ showed virescence symptoms, witches’ broom symptoms, or became asymptomatic after their planting in pots. Nine plants were grouped into three categories according to these symptoms, which were then employed for investigation. The phytoplasma concentration, as determined by qPCR, correlated well with the severity of symptoms. To reveal the changes in the small RNA profiles in these plants, small RNA high-throughput sequencing (HTS) was carried out. The bioinformatics comparison of the micro (mi) RNA and small interfering (si) RNA profiles of the symptomatic and asymptomatic plants showed changes, which could be correlated to some of the observed symptoms. These results complement previous studies on phytoplasmas and serve as a starting point for small RNA-omic studies in phytoplasma research. MDPI 2023-05-19 /pmc/articles/PMC10218541/ /pubmed/37239473 http://dx.doi.org/10.3390/genes14051114 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Contaldo, Nicoletta
Zambon, Yuri
Galbacs, Zsuszanna Nagyne
Miloro, Fabio
Havelda, Zoltan
Bertaccini, Assunta
Varallyay, Eva
Small RNA Profiling of Aster Yellows Phytoplasma-Infected Catharanthus roseus Plants Showing Different Symptoms
title Small RNA Profiling of Aster Yellows Phytoplasma-Infected Catharanthus roseus Plants Showing Different Symptoms
title_full Small RNA Profiling of Aster Yellows Phytoplasma-Infected Catharanthus roseus Plants Showing Different Symptoms
title_fullStr Small RNA Profiling of Aster Yellows Phytoplasma-Infected Catharanthus roseus Plants Showing Different Symptoms
title_full_unstemmed Small RNA Profiling of Aster Yellows Phytoplasma-Infected Catharanthus roseus Plants Showing Different Symptoms
title_short Small RNA Profiling of Aster Yellows Phytoplasma-Infected Catharanthus roseus Plants Showing Different Symptoms
title_sort small rna profiling of aster yellows phytoplasma-infected catharanthus roseus plants showing different symptoms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218541/
https://www.ncbi.nlm.nih.gov/pubmed/37239473
http://dx.doi.org/10.3390/genes14051114
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