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A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana

Abscisic acid (ABA) plays an important role in the response of plants to drought stress. However, the chemical structure of ABA is unstable, which severely limits its application in agricultural production. Here, we report the identification of a small molecule compound of tetrazolium as an ABA anal...

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Autores principales: Liu, Ruiqi, Liang, Guoyan, Gong, Jiaxin, Wang, Jiali, Zhang, Yanjie, Hao, Zhiqiang, Li, Guanglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218568/
https://www.ncbi.nlm.nih.gov/pubmed/37240123
http://dx.doi.org/10.3390/ijms24108783
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author Liu, Ruiqi
Liang, Guoyan
Gong, Jiaxin
Wang, Jiali
Zhang, Yanjie
Hao, Zhiqiang
Li, Guanglin
author_facet Liu, Ruiqi
Liang, Guoyan
Gong, Jiaxin
Wang, Jiali
Zhang, Yanjie
Hao, Zhiqiang
Li, Guanglin
author_sort Liu, Ruiqi
collection PubMed
description Abscisic acid (ABA) plays an important role in the response of plants to drought stress. However, the chemical structure of ABA is unstable, which severely limits its application in agricultural production. Here, we report the identification of a small molecule compound of tetrazolium as an ABA analog (named SLG1) through virtual screening. SLG1 inhibits the seedling growth and promotes drought resistance of Arabidopsis thaliana with higher stability. Yeast two-hybrid and PP2C inhibition assays show that SLG1 acts as a potent activator of multiple ABA receptors in A. thaliana. Results of molecular docking and molecular dynamics show that SLG1 mainly binds to PYL2 and PYL3 through its tetrazolium group and the combination is stable. Together, these results demonstrate that SLG1, as an ABA analogue, protects A. thaliana from drought stress. Moreover, the newly identified tetrazolium group of SLG1 that binds to ABA receptors can be used as a new option for structural modification of ABA analogs.
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spelling pubmed-102185682023-05-27 A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana Liu, Ruiqi Liang, Guoyan Gong, Jiaxin Wang, Jiali Zhang, Yanjie Hao, Zhiqiang Li, Guanglin Int J Mol Sci Article Abscisic acid (ABA) plays an important role in the response of plants to drought stress. However, the chemical structure of ABA is unstable, which severely limits its application in agricultural production. Here, we report the identification of a small molecule compound of tetrazolium as an ABA analog (named SLG1) through virtual screening. SLG1 inhibits the seedling growth and promotes drought resistance of Arabidopsis thaliana with higher stability. Yeast two-hybrid and PP2C inhibition assays show that SLG1 acts as a potent activator of multiple ABA receptors in A. thaliana. Results of molecular docking and molecular dynamics show that SLG1 mainly binds to PYL2 and PYL3 through its tetrazolium group and the combination is stable. Together, these results demonstrate that SLG1, as an ABA analogue, protects A. thaliana from drought stress. Moreover, the newly identified tetrazolium group of SLG1 that binds to ABA receptors can be used as a new option for structural modification of ABA analogs. MDPI 2023-05-15 /pmc/articles/PMC10218568/ /pubmed/37240123 http://dx.doi.org/10.3390/ijms24108783 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Ruiqi
Liang, Guoyan
Gong, Jiaxin
Wang, Jiali
Zhang, Yanjie
Hao, Zhiqiang
Li, Guanglin
A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana
title A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana
title_full A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana
title_fullStr A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana
title_full_unstemmed A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana
title_short A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana
title_sort potential aba analog to increase drought tolerance in arabidopsis thaliana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218568/
https://www.ncbi.nlm.nih.gov/pubmed/37240123
http://dx.doi.org/10.3390/ijms24108783
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