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Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing

The existing treatment modalities for skin injuries mainly include dressings, negative-pressure wound treatment, autologous skin grafting, and high-pressure wound treatment. All of these therapies have limitations such as high time cost, the inability to remove inactivated tissue in a timely manner,...

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Autores principales: Kou, Zheng, Li, Balun, Aierken, Aili, Tan, Ning, Li, Chenchen, Han, Miao, Jing, Yuanxiang, Li, Na, Zhang, Shiqiang, Peng, Sha, Zhao, Xianjun, Hua, Jinlian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218687/
https://www.ncbi.nlm.nih.gov/pubmed/37240027
http://dx.doi.org/10.3390/ijms24108688
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author Kou, Zheng
Li, Balun
Aierken, Aili
Tan, Ning
Li, Chenchen
Han, Miao
Jing, Yuanxiang
Li, Na
Zhang, Shiqiang
Peng, Sha
Zhao, Xianjun
Hua, Jinlian
author_facet Kou, Zheng
Li, Balun
Aierken, Aili
Tan, Ning
Li, Chenchen
Han, Miao
Jing, Yuanxiang
Li, Na
Zhang, Shiqiang
Peng, Sha
Zhao, Xianjun
Hua, Jinlian
author_sort Kou, Zheng
collection PubMed
description The existing treatment modalities for skin injuries mainly include dressings, negative-pressure wound treatment, autologous skin grafting, and high-pressure wound treatment. All of these therapies have limitations such as high time cost, the inability to remove inactivated tissue in a timely manner, surgical debridement, and oxygen toxicity. Mesenchymal stem cells have a unique self-renewal ability and wide differentiation potential, and they are one of the most promising stem cell types in cell therapy and have great application prospects in the field of regenerative medicine. Collagen exerts structural roles by promoting the molecular structure, shape, and mechanical properties of cells, and adding it to cell cultures can also promote cell proliferation and shorten the cell doubling time. The effects of collagen on MSCs were examined using Giemsa staining, EdU staining, and growth curves. Mice were subjected to allogeneic experiments and autologous experiments to reduce individual differences; all animals were separated into four groups. Neonatal skin sections were detected by HE staining, Masson staining, immunohistochemical staining, and immunofluorescence staining. We found that the MSCs pretreated with collagen accelerated the healing of skin wounds in mice and canines by promoting epidermal layer repair, collagen deposition, hair follicle angiogenesis, and an inflammatory response. Collagen promotes the secretion of the chemokines and growth factors associated with skin healing by MSCs, which positively influences skin healing. This study supports the treatment of skin injuries with MSCs cultured in medium with collagen added.
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spelling pubmed-102186872023-05-27 Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing Kou, Zheng Li, Balun Aierken, Aili Tan, Ning Li, Chenchen Han, Miao Jing, Yuanxiang Li, Na Zhang, Shiqiang Peng, Sha Zhao, Xianjun Hua, Jinlian Int J Mol Sci Article The existing treatment modalities for skin injuries mainly include dressings, negative-pressure wound treatment, autologous skin grafting, and high-pressure wound treatment. All of these therapies have limitations such as high time cost, the inability to remove inactivated tissue in a timely manner, surgical debridement, and oxygen toxicity. Mesenchymal stem cells have a unique self-renewal ability and wide differentiation potential, and they are one of the most promising stem cell types in cell therapy and have great application prospects in the field of regenerative medicine. Collagen exerts structural roles by promoting the molecular structure, shape, and mechanical properties of cells, and adding it to cell cultures can also promote cell proliferation and shorten the cell doubling time. The effects of collagen on MSCs were examined using Giemsa staining, EdU staining, and growth curves. Mice were subjected to allogeneic experiments and autologous experiments to reduce individual differences; all animals were separated into four groups. Neonatal skin sections were detected by HE staining, Masson staining, immunohistochemical staining, and immunofluorescence staining. We found that the MSCs pretreated with collagen accelerated the healing of skin wounds in mice and canines by promoting epidermal layer repair, collagen deposition, hair follicle angiogenesis, and an inflammatory response. Collagen promotes the secretion of the chemokines and growth factors associated with skin healing by MSCs, which positively influences skin healing. This study supports the treatment of skin injuries with MSCs cultured in medium with collagen added. MDPI 2023-05-12 /pmc/articles/PMC10218687/ /pubmed/37240027 http://dx.doi.org/10.3390/ijms24108688 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kou, Zheng
Li, Balun
Aierken, Aili
Tan, Ning
Li, Chenchen
Han, Miao
Jing, Yuanxiang
Li, Na
Zhang, Shiqiang
Peng, Sha
Zhao, Xianjun
Hua, Jinlian
Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing
title Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing
title_full Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing
title_fullStr Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing
title_full_unstemmed Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing
title_short Mesenchymal Stem Cells Pretreated with Collagen Promote Skin Wound-Healing
title_sort mesenchymal stem cells pretreated with collagen promote skin wound-healing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218687/
https://www.ncbi.nlm.nih.gov/pubmed/37240027
http://dx.doi.org/10.3390/ijms24108688
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