Cargando…
Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion
Tissue slicing-assisted digestion (TSAD) of adult cardiomyocytes has shown significant improvements over conventional chunk methods. However, it remains unclear how this method compares to Langendorff perfusion, the current standard of adult cardiomyocyte isolation. Using adult Bama minipigs, we per...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218724/ https://www.ncbi.nlm.nih.gov/pubmed/37235559 http://dx.doi.org/10.1371/journal.pone.0285169 |
_version_ | 1785048841849929728 |
---|---|
author | Shi, Xun Tang, Xiaoli Yao, Fang Wang, Le Zhang, Mingzhi Wang, Xin Yue, Guangxin Wang, Li Hu, Shengshou Zhou, Bingying |
author_facet | Shi, Xun Tang, Xiaoli Yao, Fang Wang, Le Zhang, Mingzhi Wang, Xin Yue, Guangxin Wang, Li Hu, Shengshou Zhou, Bingying |
author_sort | Shi, Xun |
collection | PubMed |
description | Tissue slicing-assisted digestion (TSAD) of adult cardiomyocytes has shown significant improvements over conventional chunk methods. However, it remains unclear how this method compares to Langendorff perfusion, the current standard of adult cardiomyocyte isolation. Using adult Bama minipigs, we performed cardiomyocyte isolation via these two distinct methods, and compared the resulting cellular quality, including viability, cellular structure, gene expression, and electrophysiological properties, of cardiomyocytes from 3 distinct anatomical regions, namely the left ventricle, right ventricle, and left atrial appendage. Our results revealed largely indistinguishable cell quality in all of the measured parameters. These findings suggest that that TSAD can be reliably used to isolate adult mammalian cardiomyocytes as a reliable alternative to perfusion in cardiomyocyte isolation from larger mammals, particularly when Langendorff perfusion is not feasible. |
format | Online Article Text |
id | pubmed-10218724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-102187242023-05-27 Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion Shi, Xun Tang, Xiaoli Yao, Fang Wang, Le Zhang, Mingzhi Wang, Xin Yue, Guangxin Wang, Li Hu, Shengshou Zhou, Bingying PLoS One Research Article Tissue slicing-assisted digestion (TSAD) of adult cardiomyocytes has shown significant improvements over conventional chunk methods. However, it remains unclear how this method compares to Langendorff perfusion, the current standard of adult cardiomyocyte isolation. Using adult Bama minipigs, we performed cardiomyocyte isolation via these two distinct methods, and compared the resulting cellular quality, including viability, cellular structure, gene expression, and electrophysiological properties, of cardiomyocytes from 3 distinct anatomical regions, namely the left ventricle, right ventricle, and left atrial appendage. Our results revealed largely indistinguishable cell quality in all of the measured parameters. These findings suggest that that TSAD can be reliably used to isolate adult mammalian cardiomyocytes as a reliable alternative to perfusion in cardiomyocyte isolation from larger mammals, particularly when Langendorff perfusion is not feasible. Public Library of Science 2023-05-26 /pmc/articles/PMC10218724/ /pubmed/37235559 http://dx.doi.org/10.1371/journal.pone.0285169 Text en © 2023 Shi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shi, Xun Tang, Xiaoli Yao, Fang Wang, Le Zhang, Mingzhi Wang, Xin Yue, Guangxin Wang, Li Hu, Shengshou Zhou, Bingying Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion |
title | Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion |
title_full | Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion |
title_fullStr | Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion |
title_full_unstemmed | Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion |
title_short | Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion |
title_sort | isolation of porcine adult cardiomyocytes: comparison between langendorff perfusion and tissue slicing-assisted enzyme digestion |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218724/ https://www.ncbi.nlm.nih.gov/pubmed/37235559 http://dx.doi.org/10.1371/journal.pone.0285169 |
work_keys_str_mv | AT shixun isolationofporcineadultcardiomyocytescomparisonbetweenlangendorffperfusionandtissueslicingassistedenzymedigestion AT tangxiaoli isolationofporcineadultcardiomyocytescomparisonbetweenlangendorffperfusionandtissueslicingassistedenzymedigestion AT yaofang isolationofporcineadultcardiomyocytescomparisonbetweenlangendorffperfusionandtissueslicingassistedenzymedigestion AT wangle isolationofporcineadultcardiomyocytescomparisonbetweenlangendorffperfusionandtissueslicingassistedenzymedigestion AT zhangmingzhi isolationofporcineadultcardiomyocytescomparisonbetweenlangendorffperfusionandtissueslicingassistedenzymedigestion AT wangxin isolationofporcineadultcardiomyocytescomparisonbetweenlangendorffperfusionandtissueslicingassistedenzymedigestion AT yueguangxin isolationofporcineadultcardiomyocytescomparisonbetweenlangendorffperfusionandtissueslicingassistedenzymedigestion AT wangli isolationofporcineadultcardiomyocytescomparisonbetweenlangendorffperfusionandtissueslicingassistedenzymedigestion AT hushengshou isolationofporcineadultcardiomyocytescomparisonbetweenlangendorffperfusionandtissueslicingassistedenzymedigestion AT zhoubingying isolationofporcineadultcardiomyocytescomparisonbetweenlangendorffperfusionandtissueslicingassistedenzymedigestion |