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Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion

Tissue slicing-assisted digestion (TSAD) of adult cardiomyocytes has shown significant improvements over conventional chunk methods. However, it remains unclear how this method compares to Langendorff perfusion, the current standard of adult cardiomyocyte isolation. Using adult Bama minipigs, we per...

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Autores principales: Shi, Xun, Tang, Xiaoli, Yao, Fang, Wang, Le, Zhang, Mingzhi, Wang, Xin, Yue, Guangxin, Wang, Li, Hu, Shengshou, Zhou, Bingying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218724/
https://www.ncbi.nlm.nih.gov/pubmed/37235559
http://dx.doi.org/10.1371/journal.pone.0285169
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author Shi, Xun
Tang, Xiaoli
Yao, Fang
Wang, Le
Zhang, Mingzhi
Wang, Xin
Yue, Guangxin
Wang, Li
Hu, Shengshou
Zhou, Bingying
author_facet Shi, Xun
Tang, Xiaoli
Yao, Fang
Wang, Le
Zhang, Mingzhi
Wang, Xin
Yue, Guangxin
Wang, Li
Hu, Shengshou
Zhou, Bingying
author_sort Shi, Xun
collection PubMed
description Tissue slicing-assisted digestion (TSAD) of adult cardiomyocytes has shown significant improvements over conventional chunk methods. However, it remains unclear how this method compares to Langendorff perfusion, the current standard of adult cardiomyocyte isolation. Using adult Bama minipigs, we performed cardiomyocyte isolation via these two distinct methods, and compared the resulting cellular quality, including viability, cellular structure, gene expression, and electrophysiological properties, of cardiomyocytes from 3 distinct anatomical regions, namely the left ventricle, right ventricle, and left atrial appendage. Our results revealed largely indistinguishable cell quality in all of the measured parameters. These findings suggest that that TSAD can be reliably used to isolate adult mammalian cardiomyocytes as a reliable alternative to perfusion in cardiomyocyte isolation from larger mammals, particularly when Langendorff perfusion is not feasible.
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spelling pubmed-102187242023-05-27 Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion Shi, Xun Tang, Xiaoli Yao, Fang Wang, Le Zhang, Mingzhi Wang, Xin Yue, Guangxin Wang, Li Hu, Shengshou Zhou, Bingying PLoS One Research Article Tissue slicing-assisted digestion (TSAD) of adult cardiomyocytes has shown significant improvements over conventional chunk methods. However, it remains unclear how this method compares to Langendorff perfusion, the current standard of adult cardiomyocyte isolation. Using adult Bama minipigs, we performed cardiomyocyte isolation via these two distinct methods, and compared the resulting cellular quality, including viability, cellular structure, gene expression, and electrophysiological properties, of cardiomyocytes from 3 distinct anatomical regions, namely the left ventricle, right ventricle, and left atrial appendage. Our results revealed largely indistinguishable cell quality in all of the measured parameters. These findings suggest that that TSAD can be reliably used to isolate adult mammalian cardiomyocytes as a reliable alternative to perfusion in cardiomyocyte isolation from larger mammals, particularly when Langendorff perfusion is not feasible. Public Library of Science 2023-05-26 /pmc/articles/PMC10218724/ /pubmed/37235559 http://dx.doi.org/10.1371/journal.pone.0285169 Text en © 2023 Shi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shi, Xun
Tang, Xiaoli
Yao, Fang
Wang, Le
Zhang, Mingzhi
Wang, Xin
Yue, Guangxin
Wang, Li
Hu, Shengshou
Zhou, Bingying
Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion
title Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion
title_full Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion
title_fullStr Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion
title_full_unstemmed Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion
title_short Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion
title_sort isolation of porcine adult cardiomyocytes: comparison between langendorff perfusion and tissue slicing-assisted enzyme digestion
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218724/
https://www.ncbi.nlm.nih.gov/pubmed/37235559
http://dx.doi.org/10.1371/journal.pone.0285169
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