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Isolation of porcine adult cardiomyocytes: Comparison between Langendorff perfusion and tissue slicing-assisted enzyme digestion

Tissue slicing-assisted digestion (TSAD) of adult cardiomyocytes has shown significant improvements over conventional chunk methods. However, it remains unclear how this method compares to Langendorff perfusion, the current standard of adult cardiomyocyte isolation. Using adult Bama minipigs, we per...

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Detalles Bibliográficos
Autores principales: Shi, Xun, Tang, Xiaoli, Yao, Fang, Wang, Le, Zhang, Mingzhi, Wang, Xin, Yue, Guangxin, Wang, Li, Hu, Shengshou, Zhou, Bingying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218724/
https://www.ncbi.nlm.nih.gov/pubmed/37235559
http://dx.doi.org/10.1371/journal.pone.0285169