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Putative Wound Healing Induction Functions of Exosomes Isolated from IMMUNEPOTENT CRP

Chronic wounds in diabetic patients can take months or years to heal, representing a great cost for the healthcare sector and impacts on patients’ lifestyles. Therefore, new effective treatment alternatives are needed to accelerate the healing process. Exosomes are nanovesicles involved in the modul...

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Autores principales: García Coronado, Paola Leonor, Franco Molina, Moisés Armides, Zárate Triviño, Diana Ginette, Menchaca Arredondo, Jorge Luis, Zapata Benavides, Pablo, Rodriguez Padilla, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218804/
https://www.ncbi.nlm.nih.gov/pubmed/37240318
http://dx.doi.org/10.3390/ijms24108971
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author García Coronado, Paola Leonor
Franco Molina, Moisés Armides
Zárate Triviño, Diana Ginette
Menchaca Arredondo, Jorge Luis
Zapata Benavides, Pablo
Rodriguez Padilla, Cristina
author_facet García Coronado, Paola Leonor
Franco Molina, Moisés Armides
Zárate Triviño, Diana Ginette
Menchaca Arredondo, Jorge Luis
Zapata Benavides, Pablo
Rodriguez Padilla, Cristina
author_sort García Coronado, Paola Leonor
collection PubMed
description Chronic wounds in diabetic patients can take months or years to heal, representing a great cost for the healthcare sector and impacts on patients’ lifestyles. Therefore, new effective treatment alternatives are needed to accelerate the healing process. Exosomes are nanovesicles involved in the modulation of signaling pathways that can be produced by any cell and can exert functions similar to the cell of origin. For this reason, IMMUNEPOTENT CRP, which is a bovine spleen leukocyte extract, was analyzed to identify the proteins present and is proposed as a source of exosomes. The exosomes were isolated through ultracentrifugation and shape-size, characterized by atomic force microscopy. The protein content in IMMUNEPOTENT CRP was characterized by EV-trap coupled to liquid chromatography. The in silico analyses for biological pathways, tissue specificity, and transcription factor inducement were performed in GOrilla ontology, Panther ontology, Metascape, and Reactome. It was observed that IMMUNEPOTENT CRP contains diverse peptides. The peptide-containing exosomes had an average size of 60 nm, and exomeres of 30 nm. They had biological activity capable of modulating the wound healing process, through inflammation modulation and the activation of signaling pathways such as PIP3-AKT, as well as other pathways activated by FOXE genes related to specificity in the skin tissue.
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spelling pubmed-102188042023-05-27 Putative Wound Healing Induction Functions of Exosomes Isolated from IMMUNEPOTENT CRP García Coronado, Paola Leonor Franco Molina, Moisés Armides Zárate Triviño, Diana Ginette Menchaca Arredondo, Jorge Luis Zapata Benavides, Pablo Rodriguez Padilla, Cristina Int J Mol Sci Article Chronic wounds in diabetic patients can take months or years to heal, representing a great cost for the healthcare sector and impacts on patients’ lifestyles. Therefore, new effective treatment alternatives are needed to accelerate the healing process. Exosomes are nanovesicles involved in the modulation of signaling pathways that can be produced by any cell and can exert functions similar to the cell of origin. For this reason, IMMUNEPOTENT CRP, which is a bovine spleen leukocyte extract, was analyzed to identify the proteins present and is proposed as a source of exosomes. The exosomes were isolated through ultracentrifugation and shape-size, characterized by atomic force microscopy. The protein content in IMMUNEPOTENT CRP was characterized by EV-trap coupled to liquid chromatography. The in silico analyses for biological pathways, tissue specificity, and transcription factor inducement were performed in GOrilla ontology, Panther ontology, Metascape, and Reactome. It was observed that IMMUNEPOTENT CRP contains diverse peptides. The peptide-containing exosomes had an average size of 60 nm, and exomeres of 30 nm. They had biological activity capable of modulating the wound healing process, through inflammation modulation and the activation of signaling pathways such as PIP3-AKT, as well as other pathways activated by FOXE genes related to specificity in the skin tissue. MDPI 2023-05-18 /pmc/articles/PMC10218804/ /pubmed/37240318 http://dx.doi.org/10.3390/ijms24108971 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
García Coronado, Paola Leonor
Franco Molina, Moisés Armides
Zárate Triviño, Diana Ginette
Menchaca Arredondo, Jorge Luis
Zapata Benavides, Pablo
Rodriguez Padilla, Cristina
Putative Wound Healing Induction Functions of Exosomes Isolated from IMMUNEPOTENT CRP
title Putative Wound Healing Induction Functions of Exosomes Isolated from IMMUNEPOTENT CRP
title_full Putative Wound Healing Induction Functions of Exosomes Isolated from IMMUNEPOTENT CRP
title_fullStr Putative Wound Healing Induction Functions of Exosomes Isolated from IMMUNEPOTENT CRP
title_full_unstemmed Putative Wound Healing Induction Functions of Exosomes Isolated from IMMUNEPOTENT CRP
title_short Putative Wound Healing Induction Functions of Exosomes Isolated from IMMUNEPOTENT CRP
title_sort putative wound healing induction functions of exosomes isolated from immunepotent crp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10218804/
https://www.ncbi.nlm.nih.gov/pubmed/37240318
http://dx.doi.org/10.3390/ijms24108971
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