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Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid

Cataracts are among the most common causes of childhood vision loss worldwide. This study seeks to identify differentially expressed proteins in the aqueous humor of pediatric cataract patients. Samples of aqueous humor were collected from pediatric and adult cataract patients and subjected to mass...

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Autores principales: Theophanous, Christos N., Wolfgeher, Donald J., Farooq, Asim V., Hilkert Rodriguez, Sarah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219433/
https://www.ncbi.nlm.nih.gov/pubmed/37240389
http://dx.doi.org/10.3390/ijms24109040
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author Theophanous, Christos N.
Wolfgeher, Donald J.
Farooq, Asim V.
Hilkert Rodriguez, Sarah
author_facet Theophanous, Christos N.
Wolfgeher, Donald J.
Farooq, Asim V.
Hilkert Rodriguez, Sarah
author_sort Theophanous, Christos N.
collection PubMed
description Cataracts are among the most common causes of childhood vision loss worldwide. This study seeks to identify differentially expressed proteins in the aqueous humor of pediatric cataract patients. Samples of aqueous humor were collected from pediatric and adult cataract patients and subjected to mass spectrometry-based proteomic analysis. Samples of pediatric cataracts were grouped by subtype and compared to adult samples. Differentially expressed proteins in each subtype were identified. Gene ontology analysis was performed using WikiPaths for each cataract subtype. Seven pediatric patients and ten adult patients were included in the study. Of the pediatric samples, all seven (100%) were male, three (43%) had traumatic cataracts, two (29%) had congenital cataracts, and two (29%) had posterior polar cataracts. Of the adult patients, seven (70%) were female and seven (70%) had predominantly nuclear sclerotic cataracts. A total of 128 proteins were upregulated in the pediatric samples, and 127 proteins were upregulated in the adult samples, with 75 proteins shared by both groups. Gene ontology analysis identified inflammatory and oxidative stress pathways as upregulated in pediatric cataracts. Inflammatory and oxidative stress mechanisms may be involved in pediatric cataract formation and warrant further investigation.
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spelling pubmed-102194332023-05-27 Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid Theophanous, Christos N. Wolfgeher, Donald J. Farooq, Asim V. Hilkert Rodriguez, Sarah Int J Mol Sci Article Cataracts are among the most common causes of childhood vision loss worldwide. This study seeks to identify differentially expressed proteins in the aqueous humor of pediatric cataract patients. Samples of aqueous humor were collected from pediatric and adult cataract patients and subjected to mass spectrometry-based proteomic analysis. Samples of pediatric cataracts were grouped by subtype and compared to adult samples. Differentially expressed proteins in each subtype were identified. Gene ontology analysis was performed using WikiPaths for each cataract subtype. Seven pediatric patients and ten adult patients were included in the study. Of the pediatric samples, all seven (100%) were male, three (43%) had traumatic cataracts, two (29%) had congenital cataracts, and two (29%) had posterior polar cataracts. Of the adult patients, seven (70%) were female and seven (70%) had predominantly nuclear sclerotic cataracts. A total of 128 proteins were upregulated in the pediatric samples, and 127 proteins were upregulated in the adult samples, with 75 proteins shared by both groups. Gene ontology analysis identified inflammatory and oxidative stress pathways as upregulated in pediatric cataracts. Inflammatory and oxidative stress mechanisms may be involved in pediatric cataract formation and warrant further investigation. MDPI 2023-05-20 /pmc/articles/PMC10219433/ /pubmed/37240389 http://dx.doi.org/10.3390/ijms24109040 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Theophanous, Christos N.
Wolfgeher, Donald J.
Farooq, Asim V.
Hilkert Rodriguez, Sarah
Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid
title Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid
title_full Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid
title_fullStr Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid
title_full_unstemmed Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid
title_short Biomarkers of Pediatric Cataracts: A Proteomics Analysis of Aqueous Fluid
title_sort biomarkers of pediatric cataracts: a proteomics analysis of aqueous fluid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219433/
https://www.ncbi.nlm.nih.gov/pubmed/37240389
http://dx.doi.org/10.3390/ijms24109040
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