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Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection
Tobacco mosaic virus (TMV) is a systemic virus that poses a serious threat to crops worldwide. In the present study, a series of novel 1-phenyl-4-(1,3,4-thiadiazole-5-thioether)-1H-pyrazole-5-amine derivatives was designed and synthesized. In vivo antiviral bioassay results indicated that some of th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219444/ https://www.ncbi.nlm.nih.gov/pubmed/37240228 http://dx.doi.org/10.3390/ijms24108881 |
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author | Zheng, Huanlin Wen, Fanglin Zhang, Chengzhi Luo, Rui Wu, Zhibing |
author_facet | Zheng, Huanlin Wen, Fanglin Zhang, Chengzhi Luo, Rui Wu, Zhibing |
author_sort | Zheng, Huanlin |
collection | PubMed |
description | Tobacco mosaic virus (TMV) is a systemic virus that poses a serious threat to crops worldwide. In the present study, a series of novel 1-phenyl-4-(1,3,4-thiadiazole-5-thioether)-1H-pyrazole-5-amine derivatives was designed and synthesized. In vivo antiviral bioassay results indicated that some of these compounds exhibited excellent protective activity against TMV. Among the compounds, E(2) (EC(50) = 203.5 μg/mL) was superior to the commercial agent ningnanmycin (EC(50) = 261.4 μg/mL). Observation of tobacco leaves infected with TMV-GFP revealed that E(2) could effectively inhibit the spread of TMV in the host. Further plant tissue morphological observation indicated that E(2) could induce the tight arrangement and alignment of the spongy mesophyll and palisade cells while causing stomatal closure to form a defensive barrier to prevent viral infection in the leaves. In addition, the chlorophyll content of tobacco leaves was significantly increased after treatment with E(2), and the net photosynthesis (Pn) value was also increased, which demonstrated that the active compound could improve the photosynthetic efficiency of TMV-infected tobacco leaves by maintaining stable chlorophyll content in the leaves, thereby protecting host plants from viral infection. The results of MDA and H(2)O(2) content determination revealed that E(2) could effectively reduce the content of peroxides in the infected plants, reducing the damage to the plants caused by oxidation. This work provides an important support for the research and development of antiviral agents in crop protection. |
format | Online Article Text |
id | pubmed-10219444 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102194442023-05-27 Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection Zheng, Huanlin Wen, Fanglin Zhang, Chengzhi Luo, Rui Wu, Zhibing Int J Mol Sci Article Tobacco mosaic virus (TMV) is a systemic virus that poses a serious threat to crops worldwide. In the present study, a series of novel 1-phenyl-4-(1,3,4-thiadiazole-5-thioether)-1H-pyrazole-5-amine derivatives was designed and synthesized. In vivo antiviral bioassay results indicated that some of these compounds exhibited excellent protective activity against TMV. Among the compounds, E(2) (EC(50) = 203.5 μg/mL) was superior to the commercial agent ningnanmycin (EC(50) = 261.4 μg/mL). Observation of tobacco leaves infected with TMV-GFP revealed that E(2) could effectively inhibit the spread of TMV in the host. Further plant tissue morphological observation indicated that E(2) could induce the tight arrangement and alignment of the spongy mesophyll and palisade cells while causing stomatal closure to form a defensive barrier to prevent viral infection in the leaves. In addition, the chlorophyll content of tobacco leaves was significantly increased after treatment with E(2), and the net photosynthesis (Pn) value was also increased, which demonstrated that the active compound could improve the photosynthetic efficiency of TMV-infected tobacco leaves by maintaining stable chlorophyll content in the leaves, thereby protecting host plants from viral infection. The results of MDA and H(2)O(2) content determination revealed that E(2) could effectively reduce the content of peroxides in the infected plants, reducing the damage to the plants caused by oxidation. This work provides an important support for the research and development of antiviral agents in crop protection. MDPI 2023-05-17 /pmc/articles/PMC10219444/ /pubmed/37240228 http://dx.doi.org/10.3390/ijms24108881 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zheng, Huanlin Wen, Fanglin Zhang, Chengzhi Luo, Rui Wu, Zhibing Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection |
title | Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection |
title_full | Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection |
title_fullStr | Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection |
title_full_unstemmed | Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection |
title_short | Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection |
title_sort | novel 1,3,4-thiadiazole derivatives: synthesis, antiviral bioassay and regulation the photosynthetic pathway of tobacco against tmv infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219444/ https://www.ncbi.nlm.nih.gov/pubmed/37240228 http://dx.doi.org/10.3390/ijms24108881 |
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