Cargando…

Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection

Tobacco mosaic virus (TMV) is a systemic virus that poses a serious threat to crops worldwide. In the present study, a series of novel 1-phenyl-4-(1,3,4-thiadiazole-5-thioether)-1H-pyrazole-5-amine derivatives was designed and synthesized. In vivo antiviral bioassay results indicated that some of th...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Huanlin, Wen, Fanglin, Zhang, Chengzhi, Luo, Rui, Wu, Zhibing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219444/
https://www.ncbi.nlm.nih.gov/pubmed/37240228
http://dx.doi.org/10.3390/ijms24108881
_version_ 1785049011696173056
author Zheng, Huanlin
Wen, Fanglin
Zhang, Chengzhi
Luo, Rui
Wu, Zhibing
author_facet Zheng, Huanlin
Wen, Fanglin
Zhang, Chengzhi
Luo, Rui
Wu, Zhibing
author_sort Zheng, Huanlin
collection PubMed
description Tobacco mosaic virus (TMV) is a systemic virus that poses a serious threat to crops worldwide. In the present study, a series of novel 1-phenyl-4-(1,3,4-thiadiazole-5-thioether)-1H-pyrazole-5-amine derivatives was designed and synthesized. In vivo antiviral bioassay results indicated that some of these compounds exhibited excellent protective activity against TMV. Among the compounds, E(2) (EC(50) = 203.5 μg/mL) was superior to the commercial agent ningnanmycin (EC(50) = 261.4 μg/mL). Observation of tobacco leaves infected with TMV-GFP revealed that E(2) could effectively inhibit the spread of TMV in the host. Further plant tissue morphological observation indicated that E(2) could induce the tight arrangement and alignment of the spongy mesophyll and palisade cells while causing stomatal closure to form a defensive barrier to prevent viral infection in the leaves. In addition, the chlorophyll content of tobacco leaves was significantly increased after treatment with E(2), and the net photosynthesis (Pn) value was also increased, which demonstrated that the active compound could improve the photosynthetic efficiency of TMV-infected tobacco leaves by maintaining stable chlorophyll content in the leaves, thereby protecting host plants from viral infection. The results of MDA and H(2)O(2) content determination revealed that E(2) could effectively reduce the content of peroxides in the infected plants, reducing the damage to the plants caused by oxidation. This work provides an important support for the research and development of antiviral agents in crop protection.
format Online
Article
Text
id pubmed-10219444
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102194442023-05-27 Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection Zheng, Huanlin Wen, Fanglin Zhang, Chengzhi Luo, Rui Wu, Zhibing Int J Mol Sci Article Tobacco mosaic virus (TMV) is a systemic virus that poses a serious threat to crops worldwide. In the present study, a series of novel 1-phenyl-4-(1,3,4-thiadiazole-5-thioether)-1H-pyrazole-5-amine derivatives was designed and synthesized. In vivo antiviral bioassay results indicated that some of these compounds exhibited excellent protective activity against TMV. Among the compounds, E(2) (EC(50) = 203.5 μg/mL) was superior to the commercial agent ningnanmycin (EC(50) = 261.4 μg/mL). Observation of tobacco leaves infected with TMV-GFP revealed that E(2) could effectively inhibit the spread of TMV in the host. Further plant tissue morphological observation indicated that E(2) could induce the tight arrangement and alignment of the spongy mesophyll and palisade cells while causing stomatal closure to form a defensive barrier to prevent viral infection in the leaves. In addition, the chlorophyll content of tobacco leaves was significantly increased after treatment with E(2), and the net photosynthesis (Pn) value was also increased, which demonstrated that the active compound could improve the photosynthetic efficiency of TMV-infected tobacco leaves by maintaining stable chlorophyll content in the leaves, thereby protecting host plants from viral infection. The results of MDA and H(2)O(2) content determination revealed that E(2) could effectively reduce the content of peroxides in the infected plants, reducing the damage to the plants caused by oxidation. This work provides an important support for the research and development of antiviral agents in crop protection. MDPI 2023-05-17 /pmc/articles/PMC10219444/ /pubmed/37240228 http://dx.doi.org/10.3390/ijms24108881 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Huanlin
Wen, Fanglin
Zhang, Chengzhi
Luo, Rui
Wu, Zhibing
Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection
title Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection
title_full Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection
title_fullStr Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection
title_full_unstemmed Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection
title_short Novel 1,3,4-Thiadiazole Derivatives: Synthesis, Antiviral Bioassay and Regulation the Photosynthetic Pathway of Tobacco against TMV Infection
title_sort novel 1,3,4-thiadiazole derivatives: synthesis, antiviral bioassay and regulation the photosynthetic pathway of tobacco against tmv infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219444/
https://www.ncbi.nlm.nih.gov/pubmed/37240228
http://dx.doi.org/10.3390/ijms24108881
work_keys_str_mv AT zhenghuanlin novel134thiadiazolederivativessynthesisantiviralbioassayandregulationthephotosyntheticpathwayoftobaccoagainsttmvinfection
AT wenfanglin novel134thiadiazolederivativessynthesisantiviralbioassayandregulationthephotosyntheticpathwayoftobaccoagainsttmvinfection
AT zhangchengzhi novel134thiadiazolederivativessynthesisantiviralbioassayandregulationthephotosyntheticpathwayoftobaccoagainsttmvinfection
AT luorui novel134thiadiazolederivativessynthesisantiviralbioassayandregulationthephotosyntheticpathwayoftobaccoagainsttmvinfection
AT wuzhibing novel134thiadiazolederivativessynthesisantiviralbioassayandregulationthephotosyntheticpathwayoftobaccoagainsttmvinfection