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Comparative pathology and immunohistochemistry of Newcastle disease in domestic chicken (Gallus-gallus domesticus) and Alabio duck (Anas platyrhynchos Borneo)

BACKGROUND: Newcastle disease is very pathogenic in chickens, whereas in ducks, the disease does not show any clinical symptoms. AIMS: To compare the clinical symptoms features, pathological lesions, viral distribution, and apoptosis response caused by the Newcastle disease virus (NDV) in domestic c...

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Autores principales: Etriwati, Etriwati, Agungpriyono, Dewi Ratih, Setiyaningsih, Surachmi, Darniati, Darniati, Ak, Daud M., Erwin, Erwin, Handharyani, Ekowati
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of Veterinary Medicine 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219819/
https://www.ncbi.nlm.nih.gov/pubmed/37251268
http://dx.doi.org/10.5455/OVJ.2023.v13.i4.5
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author Etriwati, Etriwati
Agungpriyono, Dewi Ratih
Setiyaningsih, Surachmi
Darniati, Darniati
Ak, Daud M.
Erwin, Erwin
Handharyani, Ekowati
author_facet Etriwati, Etriwati
Agungpriyono, Dewi Ratih
Setiyaningsih, Surachmi
Darniati, Darniati
Ak, Daud M.
Erwin, Erwin
Handharyani, Ekowati
author_sort Etriwati, Etriwati
collection PubMed
description BACKGROUND: Newcastle disease is very pathogenic in chickens, whereas in ducks, the disease does not show any clinical symptoms. AIMS: To compare the clinical symptoms features, pathological lesions, viral distribution, and apoptosis response caused by the Newcastle disease virus (NDV) in domestic chicken and Alabio duck. METHODS: Forty domestic chickens and 40 Alabio ducks were separated into four groups: domestic chicken and Alabio duck treatment groups, where each was infected by NDV velogenic ducks/Aceh Besar_IND/2013/eoAC080721 in 10(6)ELD(50) dosage. The domestic chicken and Alabio duck control groups were each inoculated with Phosphate Buffer Saline. The infection route was intraorbital, 0.1 ml in volume. Symptoms were observed from day 1 until day 7 post-infection (PI). Necropsy was performed on days 1, 2, 3, 5, and 7 PI for organ collection. RESULTS: Symptoms observed were disorders in the respiratory, gastrointestinal, and nervous systems, followed by 100% mortality in domestic chickens. In Alabio ducks, there were only depression and slight lethargy. The lesion in domestic chicken day 1 appeared by the lungs, thymus, Fabricius bursa, spleen, and kidney. On day 3 PI, lesions were also found in the heart, proventriculus, duodenum, and cecal tonsil. On days 5 and 7 PI, the trachea and brain lesions were found. In Alabio ducks, lesions were found in the lungs, thymus, spleen, and proventriculus on day 1. Afterward, on day 3, light lesions were found in the heart. On day 5, lesions were found in the trachea and brain; finally, on day 7, light lesions were only found in the thymus, spleen, and brain. Immunopositive reaction NDV in domestic chicken was highest in the proventriculus, duodenum, cecal tonsil, and lymphoreticular organs. In the Alabio duck, it was highest in the duodenum and cecal tonsil. The caspase-3 percentage in domestic chicken increased on day 3 PI; in Alabio ducks, on day 2 PI. CONCLUSION: Clinical symptoms and pathological lesions were faster and more severe in domestic chickens. The immunopositive reaction NDV in domestic chicken continued to increase, while in Alabio ducks, it decreased until the last observation day. Apoptosis percentage increased earlier in the Alabio duck than in domestic chicken.
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spelling pubmed-102198192023-05-28 Comparative pathology and immunohistochemistry of Newcastle disease in domestic chicken (Gallus-gallus domesticus) and Alabio duck (Anas platyrhynchos Borneo) Etriwati, Etriwati Agungpriyono, Dewi Ratih Setiyaningsih, Surachmi Darniati, Darniati Ak, Daud M. Erwin, Erwin Handharyani, Ekowati Open Vet J Original Research BACKGROUND: Newcastle disease is very pathogenic in chickens, whereas in ducks, the disease does not show any clinical symptoms. AIMS: To compare the clinical symptoms features, pathological lesions, viral distribution, and apoptosis response caused by the Newcastle disease virus (NDV) in domestic chicken and Alabio duck. METHODS: Forty domestic chickens and 40 Alabio ducks were separated into four groups: domestic chicken and Alabio duck treatment groups, where each was infected by NDV velogenic ducks/Aceh Besar_IND/2013/eoAC080721 in 10(6)ELD(50) dosage. The domestic chicken and Alabio duck control groups were each inoculated with Phosphate Buffer Saline. The infection route was intraorbital, 0.1 ml in volume. Symptoms were observed from day 1 until day 7 post-infection (PI). Necropsy was performed on days 1, 2, 3, 5, and 7 PI for organ collection. RESULTS: Symptoms observed were disorders in the respiratory, gastrointestinal, and nervous systems, followed by 100% mortality in domestic chickens. In Alabio ducks, there were only depression and slight lethargy. The lesion in domestic chicken day 1 appeared by the lungs, thymus, Fabricius bursa, spleen, and kidney. On day 3 PI, lesions were also found in the heart, proventriculus, duodenum, and cecal tonsil. On days 5 and 7 PI, the trachea and brain lesions were found. In Alabio ducks, lesions were found in the lungs, thymus, spleen, and proventriculus on day 1. Afterward, on day 3, light lesions were found in the heart. On day 5, lesions were found in the trachea and brain; finally, on day 7, light lesions were only found in the thymus, spleen, and brain. Immunopositive reaction NDV in domestic chicken was highest in the proventriculus, duodenum, cecal tonsil, and lymphoreticular organs. In the Alabio duck, it was highest in the duodenum and cecal tonsil. The caspase-3 percentage in domestic chicken increased on day 3 PI; in Alabio ducks, on day 2 PI. CONCLUSION: Clinical symptoms and pathological lesions were faster and more severe in domestic chickens. The immunopositive reaction NDV in domestic chicken continued to increase, while in Alabio ducks, it decreased until the last observation day. Apoptosis percentage increased earlier in the Alabio duck than in domestic chicken. Faculty of Veterinary Medicine 2023-04 2023-04-11 /pmc/articles/PMC10219819/ /pubmed/37251268 http://dx.doi.org/10.5455/OVJ.2023.v13.i4.5 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Etriwati, Etriwati
Agungpriyono, Dewi Ratih
Setiyaningsih, Surachmi
Darniati, Darniati
Ak, Daud M.
Erwin, Erwin
Handharyani, Ekowati
Comparative pathology and immunohistochemistry of Newcastle disease in domestic chicken (Gallus-gallus domesticus) and Alabio duck (Anas platyrhynchos Borneo)
title Comparative pathology and immunohistochemistry of Newcastle disease in domestic chicken (Gallus-gallus domesticus) and Alabio duck (Anas platyrhynchos Borneo)
title_full Comparative pathology and immunohistochemistry of Newcastle disease in domestic chicken (Gallus-gallus domesticus) and Alabio duck (Anas platyrhynchos Borneo)
title_fullStr Comparative pathology and immunohistochemistry of Newcastle disease in domestic chicken (Gallus-gallus domesticus) and Alabio duck (Anas platyrhynchos Borneo)
title_full_unstemmed Comparative pathology and immunohistochemistry of Newcastle disease in domestic chicken (Gallus-gallus domesticus) and Alabio duck (Anas platyrhynchos Borneo)
title_short Comparative pathology and immunohistochemistry of Newcastle disease in domestic chicken (Gallus-gallus domesticus) and Alabio duck (Anas platyrhynchos Borneo)
title_sort comparative pathology and immunohistochemistry of newcastle disease in domestic chicken (gallus-gallus domesticus) and alabio duck (anas platyrhynchos borneo)
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219819/
https://www.ncbi.nlm.nih.gov/pubmed/37251268
http://dx.doi.org/10.5455/OVJ.2023.v13.i4.5
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