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Split selectable marker systems utilizing inteins facilitate gene stacking in plants
The ability to stack multiple genes in plants is of great importance in the development of crops with desirable traits but can be challenging due to limited selectable marker options. Here we establish split selectable marker systems using protein splicing elements called “inteins” for Agrobacterium...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219933/ https://www.ncbi.nlm.nih.gov/pubmed/37237044 http://dx.doi.org/10.1038/s42003-023-04950-8 |
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author | Yuan, Guoliang Lu, Haiwei De, Kuntal Hassan, Md Mahmudul Liu, Yang Islam, Md. Torikul Muchero, Wellington Tuskan, Gerald A. Yang, Xiaohan |
author_facet | Yuan, Guoliang Lu, Haiwei De, Kuntal Hassan, Md Mahmudul Liu, Yang Islam, Md. Torikul Muchero, Wellington Tuskan, Gerald A. Yang, Xiaohan |
author_sort | Yuan, Guoliang |
collection | PubMed |
description | The ability to stack multiple genes in plants is of great importance in the development of crops with desirable traits but can be challenging due to limited selectable marker options. Here we establish split selectable marker systems using protein splicing elements called “inteins” for Agrobacterium-mediated co-transformation in plants. First, we show that such a split selectable marker system can be used effectively in plants to reconstitute a visible marker, RUBY, from two non-functional fragments through tobacco leaf infiltration. Next, to determine the general applicability of our split selectable marker systems, we demonstrate the utility of these systems in the model plants Arabidopsis and poplar by successfully stacking two reporters eYGFPuv and RUBY, using split Kanamycin or Hygromycin resistance markers. In conclusion, this method enables robust plant co-transformation, providing a valuable tool for the simultaneous insertion of multiple genes into both herbaceous and woody plants efficiently. |
format | Online Article Text |
id | pubmed-10219933 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-102199332023-05-28 Split selectable marker systems utilizing inteins facilitate gene stacking in plants Yuan, Guoliang Lu, Haiwei De, Kuntal Hassan, Md Mahmudul Liu, Yang Islam, Md. Torikul Muchero, Wellington Tuskan, Gerald A. Yang, Xiaohan Commun Biol Article The ability to stack multiple genes in plants is of great importance in the development of crops with desirable traits but can be challenging due to limited selectable marker options. Here we establish split selectable marker systems using protein splicing elements called “inteins” for Agrobacterium-mediated co-transformation in plants. First, we show that such a split selectable marker system can be used effectively in plants to reconstitute a visible marker, RUBY, from two non-functional fragments through tobacco leaf infiltration. Next, to determine the general applicability of our split selectable marker systems, we demonstrate the utility of these systems in the model plants Arabidopsis and poplar by successfully stacking two reporters eYGFPuv and RUBY, using split Kanamycin or Hygromycin resistance markers. In conclusion, this method enables robust plant co-transformation, providing a valuable tool for the simultaneous insertion of multiple genes into both herbaceous and woody plants efficiently. Nature Publishing Group UK 2023-05-26 /pmc/articles/PMC10219933/ /pubmed/37237044 http://dx.doi.org/10.1038/s42003-023-04950-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yuan, Guoliang Lu, Haiwei De, Kuntal Hassan, Md Mahmudul Liu, Yang Islam, Md. Torikul Muchero, Wellington Tuskan, Gerald A. Yang, Xiaohan Split selectable marker systems utilizing inteins facilitate gene stacking in plants |
title | Split selectable marker systems utilizing inteins facilitate gene stacking in plants |
title_full | Split selectable marker systems utilizing inteins facilitate gene stacking in plants |
title_fullStr | Split selectable marker systems utilizing inteins facilitate gene stacking in plants |
title_full_unstemmed | Split selectable marker systems utilizing inteins facilitate gene stacking in plants |
title_short | Split selectable marker systems utilizing inteins facilitate gene stacking in plants |
title_sort | split selectable marker systems utilizing inteins facilitate gene stacking in plants |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10219933/ https://www.ncbi.nlm.nih.gov/pubmed/37237044 http://dx.doi.org/10.1038/s42003-023-04950-8 |
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