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Characterization of plasmids carrying bla(CTX-M) genes among extra-intestinal Escherichia coli clinical isolates in Ethiopia

CTX-Ms are encoded by bla(CTX-M) genes and are widely distributed extended-spectrum β-lactamases (ESBLs). They are the most important antimicrobial resistance (AMR) mechanism to β-lactam antibiotics in the Enterobacteriaceae. However, the role of transmissible AMR plasmids in the dissemination of bl...

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Autores principales: Negeri, Abebe Aseffa, Mamo, Hassen, Gahlot, Dharmender K., Gurung, Jyoti M., Seyoum, Eyasu Tigabu, Francis, Matthew S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10220062/
https://www.ncbi.nlm.nih.gov/pubmed/37237011
http://dx.doi.org/10.1038/s41598-023-35402-2
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author Negeri, Abebe Aseffa
Mamo, Hassen
Gahlot, Dharmender K.
Gurung, Jyoti M.
Seyoum, Eyasu Tigabu
Francis, Matthew S.
author_facet Negeri, Abebe Aseffa
Mamo, Hassen
Gahlot, Dharmender K.
Gurung, Jyoti M.
Seyoum, Eyasu Tigabu
Francis, Matthew S.
author_sort Negeri, Abebe Aseffa
collection PubMed
description CTX-Ms are encoded by bla(CTX-M) genes and are widely distributed extended-spectrum β-lactamases (ESBLs). They are the most important antimicrobial resistance (AMR) mechanism to β-lactam antibiotics in the Enterobacteriaceae. However, the role of transmissible AMR plasmids in the dissemination of bla(CTX-M) genes has scarcely been studied in Africa where the burden of AMR is high and rapidly spreading. In this study, AMR plasmid transmissibility, replicon types and addiction systems were analysed in CTX-M-producing Escherichia coli clinical isolates in Ethiopia with a goal to provide molecular insight into mechanisms underlying such high prevalence and rapid dissemination. Of 100 CTX-Ms-producing isolates obtained from urine (84), pus (10) and blood (6) from four geographically distinct healthcare settings, 75% carried transmissible plasmids encoding for CTX-Ms, with CTX-M-15 being predominant (n = 51). Single IncF plasmids with the combination of F-FIA-FIB (n = 17) carried the bulk of bla(CTX-M-15) genes. In addition, IncF plasmids were associated with multiple addiction systems, ISEcp1 and various resistance phenotypes for non-cephalosporin antibiotics. Moreover, IncF plasmid carriage is associated with the international pandemic E. coli ST131 lineage. Furthermore, several CTX-M encoding plasmids were associated with serum survival of the strains, but less so with biofilm formation. Hence, both horizontal gene transfer and clonal expansion may contribute to the rapid and widespread distribution of bla(CTX-M) genes among E. coli populations in Ethiopian clinical settings. This information is relevant for local epidemiology and surveillance, but also for global understanding of the successful dissemination of AMR gene carrying plasmids.
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spelling pubmed-102200622023-05-28 Characterization of plasmids carrying bla(CTX-M) genes among extra-intestinal Escherichia coli clinical isolates in Ethiopia Negeri, Abebe Aseffa Mamo, Hassen Gahlot, Dharmender K. Gurung, Jyoti M. Seyoum, Eyasu Tigabu Francis, Matthew S. Sci Rep Article CTX-Ms are encoded by bla(CTX-M) genes and are widely distributed extended-spectrum β-lactamases (ESBLs). They are the most important antimicrobial resistance (AMR) mechanism to β-lactam antibiotics in the Enterobacteriaceae. However, the role of transmissible AMR plasmids in the dissemination of bla(CTX-M) genes has scarcely been studied in Africa where the burden of AMR is high and rapidly spreading. In this study, AMR plasmid transmissibility, replicon types and addiction systems were analysed in CTX-M-producing Escherichia coli clinical isolates in Ethiopia with a goal to provide molecular insight into mechanisms underlying such high prevalence and rapid dissemination. Of 100 CTX-Ms-producing isolates obtained from urine (84), pus (10) and blood (6) from four geographically distinct healthcare settings, 75% carried transmissible plasmids encoding for CTX-Ms, with CTX-M-15 being predominant (n = 51). Single IncF plasmids with the combination of F-FIA-FIB (n = 17) carried the bulk of bla(CTX-M-15) genes. In addition, IncF plasmids were associated with multiple addiction systems, ISEcp1 and various resistance phenotypes for non-cephalosporin antibiotics. Moreover, IncF plasmid carriage is associated with the international pandemic E. coli ST131 lineage. Furthermore, several CTX-M encoding plasmids were associated with serum survival of the strains, but less so with biofilm formation. Hence, both horizontal gene transfer and clonal expansion may contribute to the rapid and widespread distribution of bla(CTX-M) genes among E. coli populations in Ethiopian clinical settings. This information is relevant for local epidemiology and surveillance, but also for global understanding of the successful dissemination of AMR gene carrying plasmids. Nature Publishing Group UK 2023-05-26 /pmc/articles/PMC10220062/ /pubmed/37237011 http://dx.doi.org/10.1038/s41598-023-35402-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Negeri, Abebe Aseffa
Mamo, Hassen
Gahlot, Dharmender K.
Gurung, Jyoti M.
Seyoum, Eyasu Tigabu
Francis, Matthew S.
Characterization of plasmids carrying bla(CTX-M) genes among extra-intestinal Escherichia coli clinical isolates in Ethiopia
title Characterization of plasmids carrying bla(CTX-M) genes among extra-intestinal Escherichia coli clinical isolates in Ethiopia
title_full Characterization of plasmids carrying bla(CTX-M) genes among extra-intestinal Escherichia coli clinical isolates in Ethiopia
title_fullStr Characterization of plasmids carrying bla(CTX-M) genes among extra-intestinal Escherichia coli clinical isolates in Ethiopia
title_full_unstemmed Characterization of plasmids carrying bla(CTX-M) genes among extra-intestinal Escherichia coli clinical isolates in Ethiopia
title_short Characterization of plasmids carrying bla(CTX-M) genes among extra-intestinal Escherichia coli clinical isolates in Ethiopia
title_sort characterization of plasmids carrying bla(ctx-m) genes among extra-intestinal escherichia coli clinical isolates in ethiopia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10220062/
https://www.ncbi.nlm.nih.gov/pubmed/37237011
http://dx.doi.org/10.1038/s41598-023-35402-2
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