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Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes
European regulations on the control of infectious diseases provide measures to control Bovine alphaherpesvirus 1 (BoHV-1) infection in both cattle and buffalo. Owing to the reported serological cross-reactivity between BoHV-1 and Bubaline alphaherpesvirus 1 (BuHV-1), we hypothesized a new immunizati...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10220682/ https://www.ncbi.nlm.nih.gov/pubmed/37242994 http://dx.doi.org/10.3390/vaccines11050891 |
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author | Martucciello, Alessandra Balestrieri, Anna Righi, Cecilia Cappelli, Giovanna Scoccia, Eleonora Grassi, Carlo Brandi, Sergio Rossi, Elisabetta Galiero, Giorgio Gioia, Damiano Fusco, Giovanna Feliziani, Francesco De Carlo, Esterina Petrini, Stefano |
author_facet | Martucciello, Alessandra Balestrieri, Anna Righi, Cecilia Cappelli, Giovanna Scoccia, Eleonora Grassi, Carlo Brandi, Sergio Rossi, Elisabetta Galiero, Giorgio Gioia, Damiano Fusco, Giovanna Feliziani, Francesco De Carlo, Esterina Petrini, Stefano |
author_sort | Martucciello, Alessandra |
collection | PubMed |
description | European regulations on the control of infectious diseases provide measures to control Bovine alphaherpesvirus 1 (BoHV-1) infection in both cattle and buffalo. Owing to the reported serological cross-reactivity between BoHV-1 and Bubaline alphaherpesvirus 1 (BuHV-1), we hypothesized a new immunization protocol using BoHV-1 gE-deleted marker vaccines could protect water buffalo against BuHV-1. Five water buffaloes devoid of BoHV-1/BuHV-1-neutralizing antibodies were immunized with two commercial BoHV-1 gE-deleted marker vaccines at 0, 30, 210, and 240 post-vaccination days (PVDs). Five additional water buffaloes were used as controls. At 270 PVD (0 post-challenge days (PCDs), all animals were challenged intranasally with wild-type (wt) BuHV-1. The vaccinated animals produced humoral immunity (HI) as early as PVD 30 whereas, in control animals, antibodies were detected on PCD 10. After challenge infection, HI significantly increased in vaccinated animals compared to that in controls. Real-time PCR for gB revealed viral shedding in vaccinated animals from PCDs 2 to 10. In contrast, positive results were observed from PCDs 2 to 15 in the unvaccinated control group. Although the findings indicated the possible protection capabilities of the tested protocol, these findings did not support its protective roles in water buffaloes against wt-BuHV-1. |
format | Online Article Text |
id | pubmed-10220682 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102206822023-05-28 Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes Martucciello, Alessandra Balestrieri, Anna Righi, Cecilia Cappelli, Giovanna Scoccia, Eleonora Grassi, Carlo Brandi, Sergio Rossi, Elisabetta Galiero, Giorgio Gioia, Damiano Fusco, Giovanna Feliziani, Francesco De Carlo, Esterina Petrini, Stefano Vaccines (Basel) Article European regulations on the control of infectious diseases provide measures to control Bovine alphaherpesvirus 1 (BoHV-1) infection in both cattle and buffalo. Owing to the reported serological cross-reactivity between BoHV-1 and Bubaline alphaherpesvirus 1 (BuHV-1), we hypothesized a new immunization protocol using BoHV-1 gE-deleted marker vaccines could protect water buffalo against BuHV-1. Five water buffaloes devoid of BoHV-1/BuHV-1-neutralizing antibodies were immunized with two commercial BoHV-1 gE-deleted marker vaccines at 0, 30, 210, and 240 post-vaccination days (PVDs). Five additional water buffaloes were used as controls. At 270 PVD (0 post-challenge days (PCDs), all animals were challenged intranasally with wild-type (wt) BuHV-1. The vaccinated animals produced humoral immunity (HI) as early as PVD 30 whereas, in control animals, antibodies were detected on PCD 10. After challenge infection, HI significantly increased in vaccinated animals compared to that in controls. Real-time PCR for gB revealed viral shedding in vaccinated animals from PCDs 2 to 10. In contrast, positive results were observed from PCDs 2 to 15 in the unvaccinated control group. Although the findings indicated the possible protection capabilities of the tested protocol, these findings did not support its protective roles in water buffaloes against wt-BuHV-1. MDPI 2023-04-24 /pmc/articles/PMC10220682/ /pubmed/37242994 http://dx.doi.org/10.3390/vaccines11050891 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Martucciello, Alessandra Balestrieri, Anna Righi, Cecilia Cappelli, Giovanna Scoccia, Eleonora Grassi, Carlo Brandi, Sergio Rossi, Elisabetta Galiero, Giorgio Gioia, Damiano Fusco, Giovanna Feliziani, Francesco De Carlo, Esterina Petrini, Stefano Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes |
title | Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes |
title_full | Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes |
title_fullStr | Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes |
title_full_unstemmed | Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes |
title_short | Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes |
title_sort | evaluation of an immunization protocol using bovine alphaherpesvirus 1 ge-deleted marker vaccines against bubaline alphaherpesvirus 1 in water buffaloes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10220682/ https://www.ncbi.nlm.nih.gov/pubmed/37242994 http://dx.doi.org/10.3390/vaccines11050891 |
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