Cargando…

Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes

European regulations on the control of infectious diseases provide measures to control Bovine alphaherpesvirus 1 (BoHV-1) infection in both cattle and buffalo. Owing to the reported serological cross-reactivity between BoHV-1 and Bubaline alphaherpesvirus 1 (BuHV-1), we hypothesized a new immunizati...

Descripción completa

Detalles Bibliográficos
Autores principales: Martucciello, Alessandra, Balestrieri, Anna, Righi, Cecilia, Cappelli, Giovanna, Scoccia, Eleonora, Grassi, Carlo, Brandi, Sergio, Rossi, Elisabetta, Galiero, Giorgio, Gioia, Damiano, Fusco, Giovanna, Feliziani, Francesco, De Carlo, Esterina, Petrini, Stefano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10220682/
https://www.ncbi.nlm.nih.gov/pubmed/37242994
http://dx.doi.org/10.3390/vaccines11050891
_version_ 1785049276158574592
author Martucciello, Alessandra
Balestrieri, Anna
Righi, Cecilia
Cappelli, Giovanna
Scoccia, Eleonora
Grassi, Carlo
Brandi, Sergio
Rossi, Elisabetta
Galiero, Giorgio
Gioia, Damiano
Fusco, Giovanna
Feliziani, Francesco
De Carlo, Esterina
Petrini, Stefano
author_facet Martucciello, Alessandra
Balestrieri, Anna
Righi, Cecilia
Cappelli, Giovanna
Scoccia, Eleonora
Grassi, Carlo
Brandi, Sergio
Rossi, Elisabetta
Galiero, Giorgio
Gioia, Damiano
Fusco, Giovanna
Feliziani, Francesco
De Carlo, Esterina
Petrini, Stefano
author_sort Martucciello, Alessandra
collection PubMed
description European regulations on the control of infectious diseases provide measures to control Bovine alphaherpesvirus 1 (BoHV-1) infection in both cattle and buffalo. Owing to the reported serological cross-reactivity between BoHV-1 and Bubaline alphaherpesvirus 1 (BuHV-1), we hypothesized a new immunization protocol using BoHV-1 gE-deleted marker vaccines could protect water buffalo against BuHV-1. Five water buffaloes devoid of BoHV-1/BuHV-1-neutralizing antibodies were immunized with two commercial BoHV-1 gE-deleted marker vaccines at 0, 30, 210, and 240 post-vaccination days (PVDs). Five additional water buffaloes were used as controls. At 270 PVD (0 post-challenge days (PCDs), all animals were challenged intranasally with wild-type (wt) BuHV-1. The vaccinated animals produced humoral immunity (HI) as early as PVD 30 whereas, in control animals, antibodies were detected on PCD 10. After challenge infection, HI significantly increased in vaccinated animals compared to that in controls. Real-time PCR for gB revealed viral shedding in vaccinated animals from PCDs 2 to 10. In contrast, positive results were observed from PCDs 2 to 15 in the unvaccinated control group. Although the findings indicated the possible protection capabilities of the tested protocol, these findings did not support its protective roles in water buffaloes against wt-BuHV-1.
format Online
Article
Text
id pubmed-10220682
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102206822023-05-28 Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes Martucciello, Alessandra Balestrieri, Anna Righi, Cecilia Cappelli, Giovanna Scoccia, Eleonora Grassi, Carlo Brandi, Sergio Rossi, Elisabetta Galiero, Giorgio Gioia, Damiano Fusco, Giovanna Feliziani, Francesco De Carlo, Esterina Petrini, Stefano Vaccines (Basel) Article European regulations on the control of infectious diseases provide measures to control Bovine alphaherpesvirus 1 (BoHV-1) infection in both cattle and buffalo. Owing to the reported serological cross-reactivity between BoHV-1 and Bubaline alphaherpesvirus 1 (BuHV-1), we hypothesized a new immunization protocol using BoHV-1 gE-deleted marker vaccines could protect water buffalo against BuHV-1. Five water buffaloes devoid of BoHV-1/BuHV-1-neutralizing antibodies were immunized with two commercial BoHV-1 gE-deleted marker vaccines at 0, 30, 210, and 240 post-vaccination days (PVDs). Five additional water buffaloes were used as controls. At 270 PVD (0 post-challenge days (PCDs), all animals were challenged intranasally with wild-type (wt) BuHV-1. The vaccinated animals produced humoral immunity (HI) as early as PVD 30 whereas, in control animals, antibodies were detected on PCD 10. After challenge infection, HI significantly increased in vaccinated animals compared to that in controls. Real-time PCR for gB revealed viral shedding in vaccinated animals from PCDs 2 to 10. In contrast, positive results were observed from PCDs 2 to 15 in the unvaccinated control group. Although the findings indicated the possible protection capabilities of the tested protocol, these findings did not support its protective roles in water buffaloes against wt-BuHV-1. MDPI 2023-04-24 /pmc/articles/PMC10220682/ /pubmed/37242994 http://dx.doi.org/10.3390/vaccines11050891 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martucciello, Alessandra
Balestrieri, Anna
Righi, Cecilia
Cappelli, Giovanna
Scoccia, Eleonora
Grassi, Carlo
Brandi, Sergio
Rossi, Elisabetta
Galiero, Giorgio
Gioia, Damiano
Fusco, Giovanna
Feliziani, Francesco
De Carlo, Esterina
Petrini, Stefano
Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes
title Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes
title_full Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes
title_fullStr Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes
title_full_unstemmed Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes
title_short Evaluation of an Immunization Protocol Using Bovine Alphaherpesvirus 1 gE-Deleted Marker Vaccines against Bubaline Alphaherpesvirus 1 in Water Buffaloes
title_sort evaluation of an immunization protocol using bovine alphaherpesvirus 1 ge-deleted marker vaccines against bubaline alphaherpesvirus 1 in water buffaloes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10220682/
https://www.ncbi.nlm.nih.gov/pubmed/37242994
http://dx.doi.org/10.3390/vaccines11050891
work_keys_str_mv AT martuccielloalessandra evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT balestrierianna evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT righicecilia evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT cappelligiovanna evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT scocciaeleonora evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT grassicarlo evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT brandisergio evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT rossielisabetta evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT galierogiorgio evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT gioiadamiano evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT fuscogiovanna evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT felizianifrancesco evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT decarloesterina evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes
AT petrinistefano evaluationofanimmunizationprotocolusingbovinealphaherpesvirus1gedeletedmarkervaccinesagainstbubalinealphaherpesvirus1inwaterbuffaloes