Experimental Studies of the Liposomal Form of Lytic Mycobacteriophage D29 for the Treatment of Tuberculosis Infection

We have studied the antimycobacterial efficacy of the liposomal preparation of mycobacteriophage D29 on models of tuberculous granuloma in vitro and in the experiment on laboratory mice of the relatively resistant strain C57BL/6, infected with the virulent strain of M. tuberculosis H37Rv. We have sh...

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Autores principales: Avdeev, Vadim Vadimovich, Kuzin, Victor Vladimirovich, Vladimirsky, Mikhail Aleksandrovich, Vasilieva, Irina Anatol’evna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221482/
https://www.ncbi.nlm.nih.gov/pubmed/37317188
http://dx.doi.org/10.3390/microorganisms11051214
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author Avdeev, Vadim Vadimovich
Kuzin, Victor Vladimirovich
Vladimirsky, Mikhail Aleksandrovich
Vasilieva, Irina Anatol’evna
author_facet Avdeev, Vadim Vadimovich
Kuzin, Victor Vladimirovich
Vladimirsky, Mikhail Aleksandrovich
Vasilieva, Irina Anatol’evna
author_sort Avdeev, Vadim Vadimovich
collection PubMed
description We have studied the antimycobacterial efficacy of the liposomal preparation of mycobacteriophage D29 on models of tuberculous granuloma in vitro and in the experiment on laboratory mice of the relatively resistant strain C57BL/6, infected with the virulent strain of M. tuberculosis H37Rv. We have shown the preparation of liposomal preparation of the lytic mycobacteriophages and its characteristics. The experiments showed a significant lytic effect of the liposomal form of mycobacteriophage D29 both on the model of tuberculous granuloma formed by human blood mononuclear cells in vitro, which is formed in the presence of Mycobacterium tuberculosis and on the model of tuberculous infection in C57BL/6 mice. Keywords: mycobacteriophage D29, M. tuberculosis, liposomes, tuberculous granuloma in vitro, tuberculosis infection and its treatment.
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spelling pubmed-102214822023-05-28 Experimental Studies of the Liposomal Form of Lytic Mycobacteriophage D29 for the Treatment of Tuberculosis Infection Avdeev, Vadim Vadimovich Kuzin, Victor Vladimirovich Vladimirsky, Mikhail Aleksandrovich Vasilieva, Irina Anatol’evna Microorganisms Communication We have studied the antimycobacterial efficacy of the liposomal preparation of mycobacteriophage D29 on models of tuberculous granuloma in vitro and in the experiment on laboratory mice of the relatively resistant strain C57BL/6, infected with the virulent strain of M. tuberculosis H37Rv. We have shown the preparation of liposomal preparation of the lytic mycobacteriophages and its characteristics. The experiments showed a significant lytic effect of the liposomal form of mycobacteriophage D29 both on the model of tuberculous granuloma formed by human blood mononuclear cells in vitro, which is formed in the presence of Mycobacterium tuberculosis and on the model of tuberculous infection in C57BL/6 mice. Keywords: mycobacteriophage D29, M. tuberculosis, liposomes, tuberculous granuloma in vitro, tuberculosis infection and its treatment. MDPI 2023-05-05 /pmc/articles/PMC10221482/ /pubmed/37317188 http://dx.doi.org/10.3390/microorganisms11051214 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Avdeev, Vadim Vadimovich
Kuzin, Victor Vladimirovich
Vladimirsky, Mikhail Aleksandrovich
Vasilieva, Irina Anatol’evna
Experimental Studies of the Liposomal Form of Lytic Mycobacteriophage D29 for the Treatment of Tuberculosis Infection
title Experimental Studies of the Liposomal Form of Lytic Mycobacteriophage D29 for the Treatment of Tuberculosis Infection
title_full Experimental Studies of the Liposomal Form of Lytic Mycobacteriophage D29 for the Treatment of Tuberculosis Infection
title_fullStr Experimental Studies of the Liposomal Form of Lytic Mycobacteriophage D29 for the Treatment of Tuberculosis Infection
title_full_unstemmed Experimental Studies of the Liposomal Form of Lytic Mycobacteriophage D29 for the Treatment of Tuberculosis Infection
title_short Experimental Studies of the Liposomal Form of Lytic Mycobacteriophage D29 for the Treatment of Tuberculosis Infection
title_sort experimental studies of the liposomal form of lytic mycobacteriophage d29 for the treatment of tuberculosis infection
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221482/
https://www.ncbi.nlm.nih.gov/pubmed/37317188
http://dx.doi.org/10.3390/microorganisms11051214
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