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SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging

Vascular aging is linked to reduce NO bioavailability, endothelial dysfunction, oxidative stress, and inflammation. We previously showed that a 4-week treatment of middle-aged Wistar rats (MAWRs, 46 weeks old) with Moringa oleifera seed powder (MOI, 750 mg/kg/day) improved vascular function. Here, w...

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Autores principales: Conti, Valeria, Randriamboavonjy, Joseph Iharinjaka, Rafatro, Herintsoa, Manzo, Valentina, Dal Col, Jessica, Filippelli, Amelia, Corbi, Graziamaria, Tesse, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221508/
https://www.ncbi.nlm.nih.gov/pubmed/37242544
http://dx.doi.org/10.3390/ph16050761
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author Conti, Valeria
Randriamboavonjy, Joseph Iharinjaka
Rafatro, Herintsoa
Manzo, Valentina
Dal Col, Jessica
Filippelli, Amelia
Corbi, Graziamaria
Tesse, Angela
author_facet Conti, Valeria
Randriamboavonjy, Joseph Iharinjaka
Rafatro, Herintsoa
Manzo, Valentina
Dal Col, Jessica
Filippelli, Amelia
Corbi, Graziamaria
Tesse, Angela
author_sort Conti, Valeria
collection PubMed
description Vascular aging is linked to reduce NO bioavailability, endothelial dysfunction, oxidative stress, and inflammation. We previously showed that a 4-week treatment of middle-aged Wistar rats (MAWRs, 46 weeks old) with Moringa oleifera seed powder (MOI, 750 mg/kg/day) improved vascular function. Here, we investigated the involvement of SIRT1 in MOI-induced vascular improvement. MAWRs were treated with a standard or MOI-containing diet. Young rats (YWR, 16 weeks old) were the controls and received a standard diet. The hearts and aortas were harvested to evaluate SIRT1 and FOXO1 expression via Western blot and/or immunostaining, SIRT1 activity via a fluorometric assay, and oxidative stress using the DHE fluorescent probe. In the hearts and aortas, SIRT1 expression, reduced in MAWRs compared to YWRs, was enhanced in MOI MAWRs. In the hearts, SIRT1 activity did not differ between YWRs and MAWRs, whereas it was increased in MOI MAWRs compared with them. In the aortas, SIRT1 activity decreased in MAWRs, and it was similar in the MOI MAWRs and YWRs. FOXO1 expression increased in the nuclei of MAWR aortas compared to YWR and was reversed in MOI MAWRs. Interestingly, MOI treatment normalized oxidative stress enhanced in MAWRs, in both the heart and aorta. These results demonstrate the protective role of MOI against cardiovascular dysfunction due to aging via enhanced SIRT1 function and subsequently reduced oxidative stress.
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spelling pubmed-102215082023-05-28 SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging Conti, Valeria Randriamboavonjy, Joseph Iharinjaka Rafatro, Herintsoa Manzo, Valentina Dal Col, Jessica Filippelli, Amelia Corbi, Graziamaria Tesse, Angela Pharmaceuticals (Basel) Article Vascular aging is linked to reduce NO bioavailability, endothelial dysfunction, oxidative stress, and inflammation. We previously showed that a 4-week treatment of middle-aged Wistar rats (MAWRs, 46 weeks old) with Moringa oleifera seed powder (MOI, 750 mg/kg/day) improved vascular function. Here, we investigated the involvement of SIRT1 in MOI-induced vascular improvement. MAWRs were treated with a standard or MOI-containing diet. Young rats (YWR, 16 weeks old) were the controls and received a standard diet. The hearts and aortas were harvested to evaluate SIRT1 and FOXO1 expression via Western blot and/or immunostaining, SIRT1 activity via a fluorometric assay, and oxidative stress using the DHE fluorescent probe. In the hearts and aortas, SIRT1 expression, reduced in MAWRs compared to YWRs, was enhanced in MOI MAWRs. In the hearts, SIRT1 activity did not differ between YWRs and MAWRs, whereas it was increased in MOI MAWRs compared with them. In the aortas, SIRT1 activity decreased in MAWRs, and it was similar in the MOI MAWRs and YWRs. FOXO1 expression increased in the nuclei of MAWR aortas compared to YWR and was reversed in MOI MAWRs. Interestingly, MOI treatment normalized oxidative stress enhanced in MAWRs, in both the heart and aorta. These results demonstrate the protective role of MOI against cardiovascular dysfunction due to aging via enhanced SIRT1 function and subsequently reduced oxidative stress. MDPI 2023-05-18 /pmc/articles/PMC10221508/ /pubmed/37242544 http://dx.doi.org/10.3390/ph16050761 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Conti, Valeria
Randriamboavonjy, Joseph Iharinjaka
Rafatro, Herintsoa
Manzo, Valentina
Dal Col, Jessica
Filippelli, Amelia
Corbi, Graziamaria
Tesse, Angela
SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging
title SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging
title_full SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging
title_fullStr SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging
title_full_unstemmed SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging
title_short SIRT1 Signaling Is Involved in the Vascular Improvement Induced by Moringa Oleifera Seeds during Aging
title_sort sirt1 signaling is involved in the vascular improvement induced by moringa oleifera seeds during aging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221508/
https://www.ncbi.nlm.nih.gov/pubmed/37242544
http://dx.doi.org/10.3390/ph16050761
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