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Optimization of the molecular diagnosis of the acute hepatitis E virus infection

To evaluate the diagnostic value of the combination of two broad‐range PCR assays targeting two different and conserved regions of the viral genome for the diagnosis of acute Hepatitis E virus (HEV) infection. Patients with acute hepatitis were prospectively recruited. In all, HEV‐IgM antibodies wer...

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Autores principales: Lopez‐Lopez, Pedro, Frias, Mario, Perez‐Jimenez, Ana Belén, Freyre‐Carrillo, Carolina, Pineda, Juan A., Aguilera, Antonio, Fuentes, Ana, Alados, Juan Carlos, Reina, Gabriel, Ramirez‐Arellano, Encarnación, Viciana, Isabel, Mesquita, Joao, Caballero‐Gomez, Javier, Rivero‐Juarez, Antonio, Rivero, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221520/
https://www.ncbi.nlm.nih.gov/pubmed/36965117
http://dx.doi.org/10.1111/1751-7915.14247
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author Lopez‐Lopez, Pedro
Frias, Mario
Perez‐Jimenez, Ana Belén
Freyre‐Carrillo, Carolina
Pineda, Juan A.
Aguilera, Antonio
Fuentes, Ana
Alados, Juan Carlos
Reina, Gabriel
Ramirez‐Arellano, Encarnación
Viciana, Isabel
Mesquita, Joao
Caballero‐Gomez, Javier
Rivero‐Juarez, Antonio
Rivero, Antonio
author_facet Lopez‐Lopez, Pedro
Frias, Mario
Perez‐Jimenez, Ana Belén
Freyre‐Carrillo, Carolina
Pineda, Juan A.
Aguilera, Antonio
Fuentes, Ana
Alados, Juan Carlos
Reina, Gabriel
Ramirez‐Arellano, Encarnación
Viciana, Isabel
Mesquita, Joao
Caballero‐Gomez, Javier
Rivero‐Juarez, Antonio
Rivero, Antonio
author_sort Lopez‐Lopez, Pedro
collection PubMed
description To evaluate the diagnostic value of the combination of two broad‐range PCR assays targeting two different and conserved regions of the viral genome for the diagnosis of acute Hepatitis E virus (HEV) infection. Patients with acute hepatitis were prospectively recruited. In all, HEV‐IgM antibodies were tested together with evaluation of HEV viraemia by two PCR assays (ORF3 and ORF1). The number of individuals exhibiting negative IgM antibody results but carrying viral RNA was calculated by each PCR assay. Four‐hundred and seventy individuals were included, of whom 145 (30.8%) were diagnosed as having acute HEV. Of them, 122 (84.1%) exhibited HEV‐IgM antibodies, and 81 (55.8%) had detectable viral RNA for at least one PCR. Using the ORF3 molecular assay, 70 (48.3%) individuals were identified with HEV infection. When the ORF1 molecular assay was applied, 49 (33.8%) individuals were identified. The ORF3 assay detected viral RNA in 32 patients not detected by the ORF1 assay. In contrast, the ORF1 assay could amplify viral RNA in 11 patients who were not detected by the ORF3 assay. The parallel use of two broad‐range PCR assays significantly increased the performance of the molecular diagnosis of HEV.
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spelling pubmed-102215202023-05-28 Optimization of the molecular diagnosis of the acute hepatitis E virus infection Lopez‐Lopez, Pedro Frias, Mario Perez‐Jimenez, Ana Belén Freyre‐Carrillo, Carolina Pineda, Juan A. Aguilera, Antonio Fuentes, Ana Alados, Juan Carlos Reina, Gabriel Ramirez‐Arellano, Encarnación Viciana, Isabel Mesquita, Joao Caballero‐Gomez, Javier Rivero‐Juarez, Antonio Rivero, Antonio Microb Biotechnol Research Articles To evaluate the diagnostic value of the combination of two broad‐range PCR assays targeting two different and conserved regions of the viral genome for the diagnosis of acute Hepatitis E virus (HEV) infection. Patients with acute hepatitis were prospectively recruited. In all, HEV‐IgM antibodies were tested together with evaluation of HEV viraemia by two PCR assays (ORF3 and ORF1). The number of individuals exhibiting negative IgM antibody results but carrying viral RNA was calculated by each PCR assay. Four‐hundred and seventy individuals were included, of whom 145 (30.8%) were diagnosed as having acute HEV. Of them, 122 (84.1%) exhibited HEV‐IgM antibodies, and 81 (55.8%) had detectable viral RNA for at least one PCR. Using the ORF3 molecular assay, 70 (48.3%) individuals were identified with HEV infection. When the ORF1 molecular assay was applied, 49 (33.8%) individuals were identified. The ORF3 assay detected viral RNA in 32 patients not detected by the ORF1 assay. In contrast, the ORF1 assay could amplify viral RNA in 11 patients who were not detected by the ORF3 assay. The parallel use of two broad‐range PCR assays significantly increased the performance of the molecular diagnosis of HEV. John Wiley and Sons Inc. 2023-03-25 /pmc/articles/PMC10221520/ /pubmed/36965117 http://dx.doi.org/10.1111/1751-7915.14247 Text en © 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Lopez‐Lopez, Pedro
Frias, Mario
Perez‐Jimenez, Ana Belén
Freyre‐Carrillo, Carolina
Pineda, Juan A.
Aguilera, Antonio
Fuentes, Ana
Alados, Juan Carlos
Reina, Gabriel
Ramirez‐Arellano, Encarnación
Viciana, Isabel
Mesquita, Joao
Caballero‐Gomez, Javier
Rivero‐Juarez, Antonio
Rivero, Antonio
Optimization of the molecular diagnosis of the acute hepatitis E virus infection
title Optimization of the molecular diagnosis of the acute hepatitis E virus infection
title_full Optimization of the molecular diagnosis of the acute hepatitis E virus infection
title_fullStr Optimization of the molecular diagnosis of the acute hepatitis E virus infection
title_full_unstemmed Optimization of the molecular diagnosis of the acute hepatitis E virus infection
title_short Optimization of the molecular diagnosis of the acute hepatitis E virus infection
title_sort optimization of the molecular diagnosis of the acute hepatitis e virus infection
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221520/
https://www.ncbi.nlm.nih.gov/pubmed/36965117
http://dx.doi.org/10.1111/1751-7915.14247
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