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Optimization of the molecular diagnosis of the acute hepatitis E virus infection
To evaluate the diagnostic value of the combination of two broad‐range PCR assays targeting two different and conserved regions of the viral genome for the diagnosis of acute Hepatitis E virus (HEV) infection. Patients with acute hepatitis were prospectively recruited. In all, HEV‐IgM antibodies wer...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221520/ https://www.ncbi.nlm.nih.gov/pubmed/36965117 http://dx.doi.org/10.1111/1751-7915.14247 |
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author | Lopez‐Lopez, Pedro Frias, Mario Perez‐Jimenez, Ana Belén Freyre‐Carrillo, Carolina Pineda, Juan A. Aguilera, Antonio Fuentes, Ana Alados, Juan Carlos Reina, Gabriel Ramirez‐Arellano, Encarnación Viciana, Isabel Mesquita, Joao Caballero‐Gomez, Javier Rivero‐Juarez, Antonio Rivero, Antonio |
author_facet | Lopez‐Lopez, Pedro Frias, Mario Perez‐Jimenez, Ana Belén Freyre‐Carrillo, Carolina Pineda, Juan A. Aguilera, Antonio Fuentes, Ana Alados, Juan Carlos Reina, Gabriel Ramirez‐Arellano, Encarnación Viciana, Isabel Mesquita, Joao Caballero‐Gomez, Javier Rivero‐Juarez, Antonio Rivero, Antonio |
author_sort | Lopez‐Lopez, Pedro |
collection | PubMed |
description | To evaluate the diagnostic value of the combination of two broad‐range PCR assays targeting two different and conserved regions of the viral genome for the diagnosis of acute Hepatitis E virus (HEV) infection. Patients with acute hepatitis were prospectively recruited. In all, HEV‐IgM antibodies were tested together with evaluation of HEV viraemia by two PCR assays (ORF3 and ORF1). The number of individuals exhibiting negative IgM antibody results but carrying viral RNA was calculated by each PCR assay. Four‐hundred and seventy individuals were included, of whom 145 (30.8%) were diagnosed as having acute HEV. Of them, 122 (84.1%) exhibited HEV‐IgM antibodies, and 81 (55.8%) had detectable viral RNA for at least one PCR. Using the ORF3 molecular assay, 70 (48.3%) individuals were identified with HEV infection. When the ORF1 molecular assay was applied, 49 (33.8%) individuals were identified. The ORF3 assay detected viral RNA in 32 patients not detected by the ORF1 assay. In contrast, the ORF1 assay could amplify viral RNA in 11 patients who were not detected by the ORF3 assay. The parallel use of two broad‐range PCR assays significantly increased the performance of the molecular diagnosis of HEV. |
format | Online Article Text |
id | pubmed-10221520 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-102215202023-05-28 Optimization of the molecular diagnosis of the acute hepatitis E virus infection Lopez‐Lopez, Pedro Frias, Mario Perez‐Jimenez, Ana Belén Freyre‐Carrillo, Carolina Pineda, Juan A. Aguilera, Antonio Fuentes, Ana Alados, Juan Carlos Reina, Gabriel Ramirez‐Arellano, Encarnación Viciana, Isabel Mesquita, Joao Caballero‐Gomez, Javier Rivero‐Juarez, Antonio Rivero, Antonio Microb Biotechnol Research Articles To evaluate the diagnostic value of the combination of two broad‐range PCR assays targeting two different and conserved regions of the viral genome for the diagnosis of acute Hepatitis E virus (HEV) infection. Patients with acute hepatitis were prospectively recruited. In all, HEV‐IgM antibodies were tested together with evaluation of HEV viraemia by two PCR assays (ORF3 and ORF1). The number of individuals exhibiting negative IgM antibody results but carrying viral RNA was calculated by each PCR assay. Four‐hundred and seventy individuals were included, of whom 145 (30.8%) were diagnosed as having acute HEV. Of them, 122 (84.1%) exhibited HEV‐IgM antibodies, and 81 (55.8%) had detectable viral RNA for at least one PCR. Using the ORF3 molecular assay, 70 (48.3%) individuals were identified with HEV infection. When the ORF1 molecular assay was applied, 49 (33.8%) individuals were identified. The ORF3 assay detected viral RNA in 32 patients not detected by the ORF1 assay. In contrast, the ORF1 assay could amplify viral RNA in 11 patients who were not detected by the ORF3 assay. The parallel use of two broad‐range PCR assays significantly increased the performance of the molecular diagnosis of HEV. John Wiley and Sons Inc. 2023-03-25 /pmc/articles/PMC10221520/ /pubmed/36965117 http://dx.doi.org/10.1111/1751-7915.14247 Text en © 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Lopez‐Lopez, Pedro Frias, Mario Perez‐Jimenez, Ana Belén Freyre‐Carrillo, Carolina Pineda, Juan A. Aguilera, Antonio Fuentes, Ana Alados, Juan Carlos Reina, Gabriel Ramirez‐Arellano, Encarnación Viciana, Isabel Mesquita, Joao Caballero‐Gomez, Javier Rivero‐Juarez, Antonio Rivero, Antonio Optimization of the molecular diagnosis of the acute hepatitis E virus infection |
title | Optimization of the molecular diagnosis of the acute hepatitis E virus infection |
title_full | Optimization of the molecular diagnosis of the acute hepatitis E virus infection |
title_fullStr | Optimization of the molecular diagnosis of the acute hepatitis E virus infection |
title_full_unstemmed | Optimization of the molecular diagnosis of the acute hepatitis E virus infection |
title_short | Optimization of the molecular diagnosis of the acute hepatitis E virus infection |
title_sort | optimization of the molecular diagnosis of the acute hepatitis e virus infection |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10221520/ https://www.ncbi.nlm.nih.gov/pubmed/36965117 http://dx.doi.org/10.1111/1751-7915.14247 |
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